LOCUS       40924_2947       14789 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-947, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Shigaki T, Vyzasatya RR, Sivitz AB, Ward JM, Sze H, Hirschi KD.
  TITLE     The Cre-loxP recombination-based reporter system for plant 
            transcriptional expression studies
  JOURNAL   Plant Mol. Biol. 58 (1), 65-73 (2005)
  PUBMED    16028117
REFERENCE   2  (bases 1 to 14789)
  AUTHORS   Shigaki T, Hirschi KD, Ward JM, Sze H, Vyzasatya RR, Sivitz AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2004) Pediatrics, Baylor College of Medicine, Room
            9016, CNRC, 1100 Bates St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 14789)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14789)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "58"; issue: "1"; pages: "65-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2004) Pediatrics, Baylor College of Medicine, Room 9016, 
            CNRC, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(826..850)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1011..1211)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(1244..1260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3060..3648
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3936..3957
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3972..4002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4010..4026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4071..4089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4123..4156)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4168..5811
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="DAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHI
                     EVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPAN
                     DIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMY
                     TFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARD
                     PIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQ
                     SALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGL
                     TETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGY
                     VNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQ
                     HPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFV
                     DEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     terminator      5829..6081
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6105..6121)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6154..6172)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6182..6198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6340..6768
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(6811..7191)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             7296..7844
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(8111..8127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8561..8691
                     /label=Agrobacterium tumefaciens Ti plasmid left border
                     /note="Agrobacterium tumefaciens Ti plasmid left border"
     misc_feature    complement(8745..8769)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10368..11513)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11815..12606)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12798..13565
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(14258..14789)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
       61 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
      121 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
      181 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
      241 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
      301 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
      361 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
      421 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
      481 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
      541 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
      601 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
      661 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
      721 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      781 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      841 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc
      901 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
      961 tttggaactg acagaaccgc aacgttgaag gagccactca gccaattccc atcttgaaag
     1021 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa
     1081 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc
     1141 tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg
     1201 atatagctag cttagctcat cgggggatcc gatatcttaa gatcgatacc gtcgacctcg
     1261 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1321 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1381 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1441 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1501 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1561 tattctcaga atgacttggt tgagtccaaa gctagagagc tagtttcaag gatgttaaag
     1621 ttgagatcca tttttattcc caggatccac gcaacgataa cactcaatgg gaccacgaac
     1681 aaagaaatct gagttgcaga gcccaacgca acccctagag atatgtcgag cttgttcttg
     1741 aatgcgaaaa tgatggctcc agcatgctca gccgcattcc caacaatggg aagcaatatg
     1801 atgcttatga aactcactga tagtccccat gagtccgatg catcctcgat cgtgtccaca
     1861 acatactctg atagcaatgc gatgactatt gtcatcccaa cgagccaagc aaatccgctc
     1921 cagaatccta tcactggagt ttcttcaaca ctaacctcat catcatatgc atcatcatca
     1981 tcctgttgtg cctcaaacaa ttggcggtga gtccagagct ggaagatgag ataagcaatg
     2041 taagcaataa gcataactat gctgcttgtc cgcgacagag tgagcgacat tttgttaatc
     2101 atagaggtcg atacttcgcc ggttgctgca tattttaaca ataatggcag caaatgacac
     2161 aacaggccca taagcagcaa gaagaagttc acatcggctt gtttccggtc gaaccgctgc
     2221 tcgcggcgga tattggcgat accaccaaag aagagggaag tgccaagaac caagagaagg
     2281 tttgagagaa ttgaacccaa tagagagtat ttcaccactg ccactttgtt attggactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3901 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3961 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4021 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4081 cactaaaggg aacaaaagct gggtaccggg ccccccctcg agataacttc gtatagcata
     4141 cattatacga agttatttat ggtcaccgac gccaaaaaca taaagaaagg cccggcgcca
     4201 ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     4261 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga catcacttac
     4321 gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     4381 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     4441 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     4501 ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg
     4561 caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat tatcatggat
     4621 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     4681 cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa gacaattgca
     4741 ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct gcctcataga
     4801 actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     4861 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     4921 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     4981 ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac cctattctcc
     5041 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     5101 tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag gttccatctg
     5161 ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct gattacaccc
     5221 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     5281 gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga
     5341 ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     5401 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     5461 atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc tcccgctgaa
     5521 ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc aggtcttccc
     5581 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     5641 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     5701 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     5761 aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt gtaattctag
     5821 ctcgaattga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     5881 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     5941 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     6001 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     6061 tgtcatctat gttactagat ctctagaatt cctgcagccc gggggatcca ctagttctag
     6121 agcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat tacgcgcgct
     6181 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6241 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6301 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6361 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6421 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6481 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6541 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6601 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6661 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6721 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatgg
     6781 gtgatcagtt taaacaagct tgaattaatt cgatttggtg tatcgagatt ggttatgaaa
     6841 ttcagatgct agtgtaatgt attggtaatt tgggaagata taataggaag caaggctatt
     6901 tatccatttc tgaaaaggcg aaatggcgtc accgcgagcg tcacgcgcat tccgttcttg
     6961 ctgtaaagcg ttgtttggta cacttttgac tagcgaggct tggcgtgtca gcgtatctat
     7021 tcaaaagtcg ttaatggctg cggatcaaga aaaagttgga atagaaacag aatacccgcg
     7081 aaattcaggc ccggttgcca tgtcctacac gccgaaataa acgaccaaat tagtagaaaa
     7141 ataaaaactg actcggatac ttacgtcacg tcttgcgcac tgatttgaaa aatctcaata
     7201 taaacaaaga cggccacaag aaaaaaccaa aacaccgata ttcattaatc ttatctagtt
     7261 tctcaaaaaa attcatatct tccacacgtg gatccatgag cccagaacga cgcccggccg
     7321 acatccgccg tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca
     7381 tcgagacaag cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg
     7441 acctcgtccg tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg
     7501 ccggcatcgc ctacgcgggc ccctggaagg cacgcaacgc ctacgactgg acggccgagt
     7561 cgaccgtgta cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc
     7621 acctgctgaa gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc
     7681 ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc
     7741 gggcggccgg cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca
     7801 gcctgccggt accgccccgt ccggtcctgc ccgtcaccga gatctgagat cacgcgttct
     7861 aggatcctct agagtcgacc tgcaggcatg cccgctgaaa tcaccagtct ctctctacaa
     7921 atctatctct ctctataata atgtgtgagt agttcccaga taagggaatt agggttctta
     7981 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     8041 aatacttctt atcaataaaa tttctaattc ctaaaaccaa aatcccaggg taccgagctc
     8101 gaattaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8161 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8221 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     8281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     8401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8521 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     8581 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     8641 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     8701 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     8761 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     8821 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     8881 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     8941 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     9001 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
     9061 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
     9121 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
     9181 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
     9241 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
     9301 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
     9361 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
     9421 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
     9481 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
     9541 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
     9601 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
     9661 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
     9721 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
     9781 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
     9841 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
     9901 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
     9961 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
    10021 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
    10081 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    10141 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    10201 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    10261 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    10321 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    10381 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    10441 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    10501 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    10561 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    10621 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    10681 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    10741 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    10801 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    10861 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    10921 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    10981 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    11041 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    11101 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    11161 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    11221 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    11281 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    11341 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    11401 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    11461 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    11521 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    11581 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    11641 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    11701 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    11761 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    11821 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    11881 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    11941 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    12001 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    12061 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    12121 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    12181 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    12241 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    12301 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    12361 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    12421 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    12481 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    12541 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    12601 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    12661 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    12721 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    12781 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    12841 tgaggtgctc cagtggcttc tgtgtctatc agctgtccct cctgttcagc tactgacggg
    12901 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    12961 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    13021 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    13081 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    13141 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    13201 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    13261 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    13321 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    13381 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    13441 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    13501 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    13561 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    13621 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    13681 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    13741 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    13801 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    13861 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    13921 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    13981 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    14041 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    14101 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    14161 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    14221 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    14281 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    14341 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    14401 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    14461 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    14521 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    14581 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    14641 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    14701 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    14761 tccgcgaggt atccacaacg ccggcggcc
//
