LOCUS       40924_2942        4491 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-909, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Shigaki T, Vyzasatya RR, Sivitz AB, Ward JM, Sze H, Hirschi KD.
  TITLE     The Cre-loxP recombination-based reporter system for plant 
            transcriptional expression studies
  JOURNAL   Plant Mol. Biol. 58 (1), 65-73 (2005)
  PUBMED    16028117
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Shigaki T, Hirschi KD, Ward JM, Sze H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2004) Pediatrics, Baylor College of Medicine, Room
            9016, CNRC, 1100 Bates St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 4491)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4491)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "58"; issue: "1"; pages: "65-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2004) Pediatrics, Baylor College of Medicine, Room 9016, 
            CNRC, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(7..40)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             97..807
                     /codon_start=1
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     polyA_signal    925..1132
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     terminator      1204..1251
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1347..1735)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1756..2211)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2248..3105)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPFRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3106..3210)
                     /label=AmpR promoter
ORIGIN
        1 ggtaccataa cttcgtatag catacattat acgaagttat ctggaattcc ccgggctcga
       61 gaacatatgg ccatggggat ccccgggtta attaacagta aaggagaaga acttttcact
      121 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      181 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      241 actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcactta tggtgttcaa
      301 tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag tgccatgccc
      361 gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt
      421 gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat
      481 tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat
      541 gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac
      601 aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc
      661 gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa
      721 gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt
      781 acacatggca tggatgaact atacaaatag ggcgcgccac ttctaaataa gcggccgccg
      841 agatcatatc actgtggacg ttgatgaaag aatacgttat tctttcatca aatcgtggtc
      901 gacgagctcg ctgatcagcc tcgtctgtgc cttctagttg ccagccatct gttgtttgcc
      961 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     1021 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     1081 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gattctagaa
     1141 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     1201 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     1261 ggaactatat ccggatatca tgcatcgcga gagagagaga gagagagaga gagagagaga
     1321 gagagagaga gacgtgggcc caattctgtc agccgttaag tgttcctgtg tcactcaaaa
     1381 ttgctttgag aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa
     1441 ccgttaaacc ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa
     1501 aaccttagag gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc
     1561 ttagtacgtg aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat
     1621 gagggtttag ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg
     1681 aaacatgaga gcttagtacg tactatcaac aggttgaact gctgatcaac agatcctcta
     1741 cactagtgtg ccactaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     1801 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     1861 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     1921 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     1981 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2041 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2101 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2161 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     2221 cattcaggct gcacgcgttt cgaattacca atgcttaatc agtgaggcac ctatctcagc
     2281 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     2341 acggaagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     2401 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2461 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2521 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2581 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2641 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2701 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2761 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2821 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2881 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2941 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3001 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3061 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3121 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3181 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     3241 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     3301 tcgtgctagc tgtccgaaat attataaatt atcgcacaca taaaaaccat gctgttggtg
     3361 tgtctattaa atcgattttt tgttataaca gacactgctt gtccgatatt tgatttagga
     3421 tacaggtacc ggccaaatcg gccgggtggg tgttcttgct gacgttagta ggcattacac
     3481 cattggctga acgtctagga tatgccacag agcaactggc ttgttacacg ggtccaactg
     3541 ttggaggcct cttaaatgct acattcggga atgtgacaga gctgatcata tcaattttcg
     3601 ctttgaaaaa tggaatgata cgtgttgttc agctgacgtt actaggctca attctgtcta
     3661 acatgttact tgtacttggc tgcgccttct tttgtggtgg gctagtcttt taccaaaaag
     3721 accaagtctt tgacaaagga attgctactg tgaattcagg attgcttttg atggctgtca
     3781 tggggatact cttcccggct gttcttcact acacccatag cgaggttcat gctggatcat
     3841 cagagctagc cctgtcaagg ttcagcagtt gcataatgct tatagcttat gctgcttacc
     3901 tcttcttcca gttaaagagc caatctaatt cttatagccc tctcgatgag gaatcaaatc
     3961 agaacgaaga aacttctgcc gaagatgaag atcctgagat ctccaagtgg gaagctatca
     4021 tttggctttc aatattgact gcttgggtct ctcttctttc tggctatctt gttgatgcca
     4081 tagagggtgc ctcagtctca tggaatatac caatagcttt tatcagtacc atcttgcttc
     4141 ctatagttgg gaacgcagct gaacatgcag gggctatcat gtttgccatg aaagacaagc
     4201 tggatctgtc tttgggagtg gctattgggt cctcgatcca gatttccatg tttgcggtcc
     4261 cattctgcgt ggtgattgga tggatgatgg gtcaacagat ggaccttaac ttccagcttt
     4321 ttgagacggc gatgctgttc atcactgtta tagtagtagc attttttctc caggaagggt
     4381 catcgaatta cttcaaagga ttgatgctga ttctttgtta tttgatagta gctgccagtt
     4441 tctttgtaca cgaagatcct caccaagatg gtatatgagg ccataagggc c
//
