LOCUS       40924_2937       14999 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-905, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14999)
  AUTHORS   Shigaki T, Vyzasatya RR, Sivitz AB, Ward JM, Sze H, Hirschi KD.
  TITLE     The Cre-loxP recombination-based reporter system for plant 
            transcriptional expression studies
  JOURNAL   Plant Mol. Biol. 58 (1), 65-73 (2005)
  PUBMED    16028117
REFERENCE   2  (bases 1 to 14999)
  AUTHORS   Shigaki T, Hirschi KD, Ward JM, Sze H, Vyzasatya RR, Sivitz AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2004) Pediatrics, Baylor College of Medicine, Room
            9016, CNRC, 1100 Bates St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 14999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "58"; issue: "1"; pages: "65-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2004) Pediatrics, Baylor College of Medicine, Room 9016, 
            CNRC, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(826..850)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1011..1211)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(1244..1260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3060..3648
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3936..3957
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3972..4002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4010..4026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4071..4089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4123..4156)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4159..5967
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      6041..6293
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6315..6331)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6364..6382)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6392..6408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6550..6978
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(7021..7401)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             7506..8054
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(8321..8337)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8771..8901
                     /label=Agrobacterium tumefaciens Ti plasmid left border
                     /note="Agrobacterium tumefaciens Ti plasmid left border"
     misc_feature    complement(8955..8979)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10578..11723)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12025..12816)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13008..13775
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(14468..14999)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
       61 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
      121 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
      181 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
      241 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
      301 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
      361 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
      421 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
      481 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
      541 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
      601 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
      661 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
      721 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      781 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      841 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc
      901 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
      961 tttggaactg acagaaccgc aacgttgaag gagccactca gccaattccc atcttgaaag
     1021 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa
     1081 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc
     1141 tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg
     1201 atatagctag cttagctcat cgggggatcc gatatcttaa gatcgatacc gtcgacctcg
     1261 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1321 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1381 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1441 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1501 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1561 tattctcaga atgacttggt tgagtccaaa gctagagagc tagtttcaag gatgttaaag
     1621 ttgagatcca tttttattcc caggatccac gcaacgataa cactcaatgg gaccacgaac
     1681 aaagaaatct gagttgcaga gcccaacgca acccctagag atatgtcgag cttgttcttg
     1741 aatgcgaaaa tgatggctcc agcatgctca gccgcattcc caacaatggg aagcaatatg
     1801 atgcttatga aactcactga tagtccccat gagtccgatg catcctcgat cgtgtccaca
     1861 acatactctg atagcaatgc gatgactatt gtcatcccaa cgagccaagc aaatccgctc
     1921 cagaatccta tcactggagt ttcttcaaca ctaacctcat catcatatgc atcatcatca
     1981 tcctgttgtg cctcaaacaa ttggcggtga gtccagagct ggaagatgag ataagcaatg
     2041 taagcaataa gcataactat gctgcttgtc cgcgacagag tgagcgacat tttgttaatc
     2101 atagaggtcg atacttcgcc ggttgctgca tattttaaca ataatggcag caaatgacac
     2161 aacaggccca taagcagcaa gaagaagttc acatcggctt gtttccggtc gaaccgctgc
     2221 tcgcggcgga tattggcgat accaccaaag aagagggaag tgccaagaac caagagaagg
     2281 tttgagagaa ttgaacccaa tagagagtat ttcaccactg ccactttgtt attggactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3901 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3961 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4021 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4081 cactaaaggg aacaaaagct gggtaccggg ccccccctcg agataacttc gtatagcata
     4141 cattatacga agttatttat gttacgtcct gtagaaaccc caacccgtga aatcaaaaaa
     4201 ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
     4261 tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
     4321 ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt
     4381 ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
     4441 ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
     4501 gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg
     4561 aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc
     4621 aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta
     4681 atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
     4741 caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt
     4801 gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg
     4861 caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
     4921 acagccaaaa gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg
     4981 gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
     5041 cgtcatgaag atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac
     5101 cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
     5161 gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct
     5221 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
     5281 tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag
     5341 ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
     5401 gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa
     5461 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
     5521 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa
     5581 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
     5641 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
     5701 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
     5761 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
     5821 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac
     5881 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
     5941 gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
     6001 gctacagcct cgggaattgc taccgagctc gaatttcccc gatcgttcaa acatttggca
     6061 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     6121 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     6181 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     6241 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     6301 cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccaatt
     6361 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6421 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6481 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6541 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6841 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6901 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6961 aaaaatttaa cgcgaatttt aacaaaatgg gtgatcagtt taaacaagct tgaattaatt
     7021 cgatttggtg tatcgagatt ggttatgaaa ttcagatgct agtgtaatgt attggtaatt
     7081 tgggaagata taataggaag caaggctatt tatccatttc tgaaaaggcg aaatggcgtc
     7141 accgcgagcg tcacgcgcat tccgttcttg ctgtaaagcg ttgtttggta cacttttgac
     7201 tagcgaggct tggcgtgtca gcgtatctat tcaaaagtcg ttaatggctg cggatcaaga
     7261 aaaagttgga atagaaacag aatacccgcg aaattcaggc ccggttgcca tgtcctacac
     7321 gccgaaataa acgaccaaat tagtagaaaa ataaaaactg actcggatac ttacgtcacg
     7381 tcttgcgcac tgatttgaaa aatctcaata taaacaaaga cggccacaag aaaaaaccaa
     7441 aacaccgata ttcattaatc ttatctagtt tctcaaaaaa attcatatct tccacacgtg
     7501 gatccatgag cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc
     7561 cggcggtctg caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg
     7621 agccgcagga accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct
     7681 ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggc ccctggaagg
     7741 cacgcaacgc ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc
     7801 ggacgggact gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct
     7861 tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg
     7921 cgctcggata tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc
     7981 atgacgtggg tttctggcag ctggacttca gcctgccggt accgccccgt ccggtcctgc
     8041 ccgtcaccga gatctgagat cacgcgttct aggatcctct agagtcgacc tgcaggcatg
     8101 cccgctgaaa tcaccagtct ctctctacaa atctatctct ctctataata atgtgtgagt
     8161 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     8221 gaaaccctta gtatgtattt gtatttgtaa aatacttctt atcaataaaa tttctaattc
     8281 ctaaaaccaa aatcccaggg taccgagctc gaattaattc actggccgtc gttttacaac
     8341 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8401 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8461 gcctgaatgg cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8521 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8581 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     8641 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8701 caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg
     8761 ccgatttcgg aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     8821 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     8881 tggtgaaaag aaaaaccacc ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     8941 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     9001 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     9061 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     9121 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     9181 gattgtaacg atgacagagc gttgctgcct gtgatcaaat atcatctccc tcgcagagat
     9241 ccgaattatc agccttctta ttcatttctc gcttaaccgt gacaggctgt cgatcttgag
     9301 aactatgccg acataatagg aaatcgctgg ataaagccgc tgaggaagct gagtggcgct
     9361 atttctttag aagtgaacgt tgacgatatc aactccccta tccattgctc accgaatggt
     9421 acaggtcggg gacccgaagt tccgactgtc ggcctgatgc atccccggct gatcgacccc
     9481 agatctgggg ctgagaaagc ccagtaagga aacaactgta ggttcgagtc gcgagatccc
     9541 ccggaaccaa aggaagtagg ttaaacccgc tccgatcagg ccgagccacg ccaggccgag
     9601 aacattggtt cctgtaggca tcgggattgg cggatcaaac actaaagcta ctggaacgag
     9661 cagaagtcct ccggccgcca gttgccaggc ggtaaaggtg agcagaggca cgggaggttg
     9721 ccacttgcgg gtcagcacgg ttccgaacgc catggaaacc gcccccgcca ggcccgctgc
     9781 gacgccgaca ggatctagcg ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt
     9841 tccgcaaata gcccccagga ccgccatcaa tcgtatcggg ctacctagca gagcggcaga
     9901 gatgaacacg accatcagcg gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag
     9961 gcggtagacc gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat
    10021 gaagatgcgc atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa
    10081 acccgccggc aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc
    10141 cacgaaaacg ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa
    10201 gaccgacagc ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa
    10261 ccgttcagcg aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat
    10321 ctctggagct ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc
    10381 tccaagccgt cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg
    10441 cagttcgtag agcgcgccgt gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca
    10501 gtgcccgctt gttcctgaaa tgccagtaaa gcgctggctg ctgaaccccc agccggaact
    10561 gaccccacaa ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc
    10621 accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc
    10681 acgcgggtgg aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc
    10741 tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac
    10801 ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg
    10861 atcaggacct ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc
    10921 agcgacaccg attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt
    10981 aggcgcgaca ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg
    11041 tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg
    11101 tactccaaca cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg
    11161 taggtgatct tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc
    11221 gggattttct tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct
    11281 cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc
    11341 tgcttcgtgt gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc
    11401 tcgccggcgg tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac
    11461 ttcgccaaac ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc
    11521 gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc
    11581 tggaccatcg agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt
    11641 gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc
    11701 ttccggtcaa acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg
    11761 gcaatgtgcc cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc
    11821 ttatcggcaa tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc
    11881 cgaatcttgc cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg
    11941 gcctgagagc caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca
    12001 tcgttgcgcc acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt
    12061 ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc
    12121 gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc
    12181 gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc
    12241 tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc
    12301 atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc aatccacatc
    12361 ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt
    12421 atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc
    12481 gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc
    12541 ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga cctttggaac
    12601 aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt
    12661 ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt
    12721 atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag
    12781 ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta
    12841 cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc
    12901 cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg
    12961 gcgtataaca tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcca
    13021 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtgtctatc
    13081 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
    13141 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
    13201 gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc
    13261 cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
    13321 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg
    13381 aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg
    13441 cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga
    13501 cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg
    13561 gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata
    13621 cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga
    13681 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
    13741 acataaaaca ctatcaataa gttggagtca ttacccaatt atgatagaat ttacaagcta
    13801 taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc tttgcccatt
    13861 ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc ttacatacgg
    13921 cgatatcttc tatataaaag atatattatc ttatcagtat tgtcaatata ttcaaggcaa
    13981 tctgcctcct catcctcttc atcctcttcg tcttggtagc tttttaaata tggcgcttca
    14041 tagagtaatt ctgtaaaggt ccaattctcg ttttcatacc tcggtataat cttacctatc
    14101 acctcaaatg gttcgctggg tttatcgcac ccccgaacac gagcacggca cccgcgacca
    14161 ctatgccaag aatgcccaag gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc
    14221 cgacggccga agtgaagggc aggccgccac ccaggccgcc gccctcactg cccggcacct
    14281 ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg
    14341 ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact gccagcgctg
    14401 ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg gatgggaggc
    14461 ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt
    14521 cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg tttaaaagca
    14581 ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca aatgctggat
    14641 tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct gtcagcactc
    14701 tgcccctcaa gtgtcaagga tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt
    14761 caataccgca gggcacttat ccccaggctt gtccacatca tctgtgggaa actcgcgtaa
    14821 aatcaggcgt tttcgccgat ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga
    14881 gcctgcccct catctgtcaa cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc
    14941 gcccctcatc tgtcagtgag ggccaagttt tccgcgaggt atccacaacg ccggcggcc
//
