LOCUS       40924_2932        3341 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-1555, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Cre-loxP recombination vectors for the expression of Riken 
            full-length plant cDNAs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Shigaki T, Kole M, Hirschi KD.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Children's Nutrition Research Center, Baylor
            College of Medicine, Room 9016, 1100 Bates St., Houston, TX 77030, 
            USA
REFERENCE   3  (bases 1 to 3341)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3341)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2005) Children's Nutrition Research Center, Baylor College 
            of Medicine, Room 9016, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(127..160)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    183..903
                     /note="stuffer DNA from Arabidopsis thaliana cation/proton 
                     exchanger CAX2"
     terminator      922..1174
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      1247..1294
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1390..1778)
                     /direction=LEFT
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     rep_origin      complement(1793..2248)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2285..3142)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3143..3247)
                     /label=AmpR promoter
ORIGIN
        1 gctagctgtc cgaaatatta taaattatcg cacacataaa aaccatgctg ttggtgtgtc
       61 tattaaatcg attttttgtt ataacagaca ctgcttgtcc gatatttgat ttaggataca
      121 ggtaccataa cttcgtatag catacattat acgaagttat ctggaattcg gccaaatcgg
      181 ccaggattgc ttttgatggc tgtcatgggg atactcttcc cggctgttct tcactacacc
      241 catagcgagg ttcatgctgg atcatcagag ctagccctgt caaggttcag cagttgcata
      301 atgcttatag cttatgctgc ttacctcttc ttccagttaa agagccaatc taattcttat
      361 agccctctcg atgaggaatc aaatcagaac gaagaaactt ctgccgaaga tgaagatcct
      421 gagatctcca agtgggaagc tatcatttgg ctttcaatat tgactgcttg ggtctctctt
      481 ctttctggct atcttgttga tgccatagag ggtgcctcag tctcatggaa tataccaata
      541 gcttttatca gtaccatctt gcttcctata gttgggaacg cagctgaaca tgcaggggct
      601 atcatgtttg ccatgaaaga caagctggat ctgtctttgg gagtggctat tgggtcctcg
      661 atccagattt ccatgtttgc ggtcccattc tgcgtggtga ttggatggat gatgggtcaa
      721 cagatggacc ttaacttcca gctttttgag acggcgatgc tgttcatcac tgttatagta
      781 gtagcatttt ttctccagga aagggtcatc gaattacttc aangattgat gctgattctt
      841 tgttatttga tagtagctgc cagtttcttt gtacacgaag atcctcacca agatggtata
      901 tgaggccata agggccagct cgatcgttca aacatttggc aataaagttt cttaagattg
      961 aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat
     1021 gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc
     1081 ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa
     1141 ttatcgcgcg cggtgtcatc tatgttacta gatcggtcta gaagatccgg ctgctaacaa
     1201 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     1261 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
     1321 tcatgcatcg cgagagagag agagagagag agagagagag agagagagag agagacgtgg
     1381 gcccaattct gtcagccgtt aagtgttcct gtgtcactga aaattgcttt gagaggctct
     1441 aagggcttct cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag
     1501 ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt
     1561 aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg
     1621 agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta
     1681 aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt
     1741 acgtactatc aacaggttga actgctgatc aacagatcct ctacactagt ctaaattgta
     1801 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     1861 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     1921 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     1981 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2041 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2101 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2161 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2221 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgca cgcgtttcga
     2281 attaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3241 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     3301 cattaaccta taaaaatagg cgtatcacga ggccctttcg t
//
