LOCUS       40924_2927       13907 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p713-1511, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13907)
  AUTHORS   Shigaki T, Vyzasatya RR, Sivitz AB, Ward JM, Sze H, Hirschi KD.
  TITLE     The Cre-loxP recombination-based reporter system for plant 
            transcriptional expression studies
  JOURNAL   Plant Mol. Biol. 58 (1), 65-73 (2005)
  PUBMED    16028117
REFERENCE   2  (bases 1 to 13907)
  AUTHORS   Shigaki T, Hirschi KD, Ward JM, Sze H, Vyzasatya RR, Sivitz AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2004) Pediatrics, Baylor College of Medicine, Room
            9016, CNRC, 1100 Bates St., Houston, TX 77030, USA
REFERENCE   3  (bases 1 to 13907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "58"; issue: "1"; pages: "65-73"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2004) Pediatrics, Baylor College of Medicine, Room 9016, 
            CNRC, 1100 Bates St., Houston, TX 77030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(826..850)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1011..1211)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(1244..1260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3060..3648
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3936..3957
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3972..4002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4010..4026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4071..4089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(4123..4156)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4159..4875
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      4949..5201
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5223..5239)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5272..5290)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5300..5316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5458..5886
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(5929..6309)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             6414..6962
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     primer_bind     complement(7229..7245)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7679..7809
                     /label=Agrobacterium tumefaciens Ti plasmid left border
                     /note="Agrobacterium tumefaciens Ti plasmid left border"
     misc_feature    complement(7863..7887)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9486..10631)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10933..11724)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11916..12683
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(13376..13907)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
       61 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
      121 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
      181 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
      241 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
      301 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
      361 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
      421 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
      481 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
      541 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
      601 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
      661 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
      721 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      781 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      841 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc
      901 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
      961 tttggaactg acagaaccgc aacgttgaag gagccactca gccaattccc atcttgaaag
     1021 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa
     1081 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc
     1141 tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg
     1201 atatagctag cttagctcat cgggggatcc gatatcttaa gatcgatacc gtcgacctcg
     1261 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1321 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1381 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1441 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1501 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1561 tattctcaga atgacttggt tgagtccaaa gctagagagc tagtttcaag gatgttaaag
     1621 ttgagatcca tttttattcc caggatccac gcaacgataa cactcaatgg gaccacgaac
     1681 aaagaaatct gagttgcaga gcccaacgca acccctagag atatgtcgag cttgttcttg
     1741 aatgcgaaaa tgatggctcc agcatgctca gccgcattcc caacaatggg aagcaatatg
     1801 atgcttatga aactcactga tagtccccat gagtccgatg catcctcgat cgtgtccaca
     1861 acatactctg atagcaatgc gatgactatt gtcatcccaa cgagccaagc aaatccgctc
     1921 cagaatccta tcactggagt ttcttcaaca ctaacctcat catcatatgc atcatcatca
     1981 tcctgttgtg cctcaaacaa ttggcggtga gtccagagct ggaagatgag ataagcaatg
     2041 taagcaataa gcataactat gctgcttgtc cgcgacagag tgagcgacat tttgttaatc
     2101 atagaggtcg atacttcgcc ggttgctgca tattttaaca ataatggcag caaatgacac
     2161 aacaggccca taagcagcaa gaagaagttc acatcggctt gtttccggtc gaaccgctgc
     2221 tcgcggcgga tattggcgat accaccaaag aagagggaag tgccaagaac caagagaagg
     2281 tttgagagaa ttgaacccaa tagagagtat ttcaccactg ccactttgtt attggactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3901 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3961 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4021 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4081 cactaaaggg aacaaaagct gggtaccggg ccccccctcg agataacttc gtatagcata
     4141 cattatacga agttatttat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4201 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     4261 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4321 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4381 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4441 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4501 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4561 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     4621 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     4681 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     4741 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     4801 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     4861 gacgagctgt acaagtaaat caacaactct cctggcgcac catcgtcggc tacagcctcg
     4921 ggaattgcta ccgagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     4981 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5041 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     5101 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     5161 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattcc tgcagcccgg
     5221 gggatccact agttctagag cggccgccac cgcggtggag ctccaattcg ccctatagtg
     5281 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     5341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     5401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     5461 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5521 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5581 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5641 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5701 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5761 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5821 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5881 cgaattttaa caaaatgggt gatcagttta aacaagcttg aattaattcg atttggtgta
     5941 tcgagattgg ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata
     6001 ataggaagca aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc
     6061 acgcgcattc cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg
     6121 gcgtgtcagc gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat
     6181 agaaacagaa tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac
     6241 gaccaaatta gtagaaaaat aaaaactgac tcggatactt acgtcacgtc ttgcgcactg
     6301 atttgaaaaa tctcaatata aacaaagacg gccacaagaa aaaaccaaaa caccgatatt
     6361 cattaatctt atctagtttc tcaaaaaaat tcatatcttc cacacgtgga tccatgagcc
     6421 cagaacgacg cccggccgac atccgccgtg ccaccgaggc ggacatgccg gcggtctgca
     6481 ccatcgtcaa ccactacatc gagacaagca cggtcaactt ccgtaccgag ccgcaggaac
     6541 cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg
     6601 aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc ctggaaggca cgcaacgcct
     6661 acgactggac ggccgagtcg accgtgtacg tctccccccg ccaccagcgg acgggactgg
     6721 gctccacgct ctacacccac ctgctgaagt ccctggaggc acagggcttc aagagcgtgg
     6781 tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg
     6841 ccccccgcgg catgctgcgg gcggccggct tcaagcacgg gaactggcat gacgtgggtt
     6901 tctggcagct ggacttcagc ctgccggtac cgccccgtcc ggtcctgccc gtcaccgaga
     6961 tctgagatca cgcgttctag gatcctctag agtcgacctg caggcatgcc cgctgaaatc
     7021 accagtctct ctctacaaat ctatctctct ctataataat gtgtgagtag ttcccagata
     7081 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     7141 atgtatttgt atttgtaaaa tacttcttat caataaaatt tctaattcct aaaaccaaaa
     7201 tcccagggta ccgagctcga attaattcac tggccgtcgt tttacaacgt cgtgactggg
     7261 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7321 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7501 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7621 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggaa
     7681 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac
     7741 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa
     7801 aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     7861 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     7921 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     7981 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     8041 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     8101 gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc gaattatcag
     8161 ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa ctatgccgac
     8221 ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat ttctttagaa
     8281 gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac aggtcgggga
     8341 cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag atctggggct
     8401 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
     8461 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
     8521 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
     8581 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
     8641 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
     8701 atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc
     8761 ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac
     8821 catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga
     8881 aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat
     8941 ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa
     9001 cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc
     9061 ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc
     9121 aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa
     9181 cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt
     9241 cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg
     9301 aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
     9361 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
     9421 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
     9481 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
     9541 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
     9601 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
     9661 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
     9721 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
     9781 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
     9841 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
     9901 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
     9961 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
    10021 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
    10081 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
    10141 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
    10201 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
    10261 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
    10321 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
    10381 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
    10441 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
    10501 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
    10561 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
    10621 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
    10681 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
    10741 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
    10801 tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc ctgagagcca
    10861 aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc gttgcgccac
    10921 atctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    10981 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    11041 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    11101 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    11161 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    11221 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    11281 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    11341 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    11401 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    11461 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    11521 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    11581 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    11641 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    11701 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    11761 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    11821 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    11881 gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac ttactgattt
    11941 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tgtctatcag ctgtccctcc
    12001 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
    12061 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
    12121 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggcaacagcc
    12181 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
    12241 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
    12301 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
    12361 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    12421 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    12481 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    12541 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    12601 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    12661 atcaataagt tggagtcatt acccaattat gatagaattt acaagctata aggttattgt
    12721 cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct atagatatat
    12781 tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg atatcttcta
    12841 tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc tgcctcctca
    12901 tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata gagtaattct
    12961 gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac ctcaaatggt
    13021 tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa
    13081 tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag
    13141 tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg
    13201 tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc
    13261 atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg
    13321 tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg
    13381 gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg
    13441 gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca
    13501 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
    13561 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
    13621 gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg
    13681 gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt
    13741 tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca
    13801 tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg
    13861 tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggcc
//
