LOCUS       V017091                 5428 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017091
VERSION     V017091
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2807..2835)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        2948..3052
                     /label="AmpR promoter"
     CDS             3053..3910
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4084..4672
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4960..4981
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4996..5026
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5034..5050
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5058..5074
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     RBS             5123..5145
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5164..5181
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             5191..5208
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             join(5221..5428,1..2441)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
ORIGIN
        1 ccaagcctct catcactacc ctactcccta agatgattgc acgcatcaac gactggtttg
       61 aggaagtgaa agctaagcgc ggcaagcgcc cgacagcctt ccagttcctg caagaaatca
      121 agccggaagc cgtagcgtac atcaccatta agaccactct ggcttgccta accagtgctg
      181 acaatacaac cgttcaggct gtagcaagcg caatcggtcg ggccattgag gacgaggctc
      241 gcttcggtcg tatccgtgac cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac
      301 tcaacaagcg cgtagggcac gtctacaaga aagcatttat gcaagttgtc gaggctgaca
      361 tgctctctaa gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta
      421 ttcatgtagg agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac
      481 accgccaaaa tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat
      541 acgctgaggc tatcgcaacc cgtgcaggtg cgctggctgg catctctctg atgttccaac
      601 cttgcgtagt tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg
      661 gtcgtcgtcc tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag
      721 acgtttacat gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa
      781 tcaacaagaa agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg
      841 aggacatccc tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga
      901 atcctgaggc tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg
      961 ctcgcaagtc tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta
     1021 accataaggc catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt
     1081 caatgttcaa cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta
     1141 aaccaatcgg taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg
     1201 tcgataaggt tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca
     1261 tggcttgcgc taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct
     1321 gcttccttgc gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact
     1381 gctcccttcc gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc
     1441 tccgagatga ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca
     1501 tctacgggat tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga
     1561 ccgataacga agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca
     1621 agctgggcac taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga
     1681 ctaagcgttc agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag
     1741 tgctggaaga taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc
     1801 cgaatcaggc tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg
     1861 tagctgcggt tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg
     1921 tcaaagataa gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc
     1981 ctgatggttt ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga
     2041 tgttcctcgg tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg
     2101 atgcacacaa acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc
     2161 accttcgtaa gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga
     2221 ttcacgactc cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg
     2281 aaactatggt tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg
     2341 ctgaccagtt gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact
     2401 tgaacctccg tgacatctta gagtcggact tcgcgttcgc gtaacgccaa atcaatacga
     2461 ctccggatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt
     2521 agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt
     2581 gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg
     2641 ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg
     2701 cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata
     2761 cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg
     2821 atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt
     2881 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4081 ttcttgagat cctttttttc tgtgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4861 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     4921 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     4981 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     5041 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct
     5101 gcaggtcgac tctagaaata attttgttta actttaagaa ggagatatac catgggcagc
     5161 agccatcatc atcatcatca cagcagcggc ctggtgccgc gcggcagcca tatgctcgag
     5221 atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg
     5281 ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag
     5341 catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa
     5401 gctggtgagg ttgcggataa cgctgccg
//
