LOCUS       40924_2912        8108 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-twhh, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Sztal TE, Sonntag C, Hall TE, Currie PD.
  TITLE     Epistatic dissection of laminin-receptor interactions in dystrophic 
            zebrafish muscle
  JOURNAL   Hum. Mol. Genet. 21 (21), 4718-4731 (2012)
  PUBMED    22859503
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Hall TE, Sztal TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2012) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 8108)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum. Mol. 
            Genet."; date: "2012"; volume: "21"; issue: "21"; pages: "4718-4731"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2012) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      689..6153
                     /label=zebrafish tiggy-winkle hedgehog (twhh) enhancer
                     /note="zebrafish tiggy-winkle hedgehog (twhh) enhancer"
                     /regulatory_class="promoter"
     protein_bind    6153..6277
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6350..6368)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6373..6389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6502..7308
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggg gtcttctcgc gcgaaccgga tggatgctgc
      721 ccaacgacca ctaccgaagc aggtgcaata acgaccgctc tcgatagcca ctgaatgagc
      781 acattggagg ttggctgtca cagctaagtg ggagacgaaa gagcgactta gtttgacggg
      841 aggtagggtc agacgcttat gagtagaggc agtctaccca tatgaattac agagcaaatc
      901 tatggcaggg ggagggagtc tattgactcg acggagagcc gtccaactat gatgccaacc
      961 cagcaaagac tgtctctata accccctcat ttctgcagaa atgacacgaa cctgttctcg
     1021 ctttgctgtg ctaaactcta gttctttggg tcaatatgaa taaaactatg caaataaagt
     1081 gtagtaatta gaagcgagca gaaaaaagaa gtaattataa agttttaaag aaaatgtgtc
     1141 tttgacagaa ccatttcaga attacattat ttcatttcaa ttggtttagt tttatgttcc
     1201 aaacaattat aaatcagact gcatgtcgat ttgtaatata taggtattga ggttaatgcg
     1261 catttggggc aacaataatt cgtcaggttt aaaaacaaat cttattaacg taacgtaata
     1321 acttattgtt tacccctttt tttggtattg ctctttttaa tttcccttct gttaatatgt
     1381 tgtgacgtgg aaaactgttc aataacgtga ttttgtaaaa ttctaatcaa atctctctct
     1441 ctctctctct ctctctctct ctctctctct ctttctctct ctctctctct ctctctctct
     1501 ctctctctct ctttctttct ctccttgaag ttttgaagca catgacccaa atgtatgctg
     1561 taatttatac tttttttcat aaggtatcaa tacctacact tcagtattta atgacactat
     1621 aaatgacttt attttattta tttatttatt tatttattta tttataaatt ctataaatag
     1681 acagtttcta tcaaatactt ttgttgagct ttgcacaatt acaaacattt taataaatca
     1741 atggtataaa tggctgtggt attttcagat tatataaatt ttttcttttc attcattctt
     1801 tcatacattc tttcattcat tcattttcct tcggtctatt tccttattta tcagagattg
     1861 ccacaacgga atgaaccgcc aacttatcca gcatttgttt gcgctgtgcg tgcccttcca
     1921 gctgcaaccc agtactggga aacacccata cactctcaca tttactcatt cactcataca
     1981 gaggaaaccc acacgaacat aagcaaactc cacacagaaa tgccaactga cccagccggg
     2041 gcgcgaagga gcgaccttgc tgtgaggcaa cagtgccaac cactgagcca ctgtgccgac
     2101 ttaatttgtc ttttcatata aaatatttta aaattaatat ttgattgtat tgcgttgatt
     2161 tttttcctct cctttttgtg ggtgggggtg ggagggaatt gtcttgggga ccacttggaa
     2221 gtttgctgag gccctctagt gggccccaga cccccatttg agaacatttt ctttaaatac
     2281 atcttttttg tttttctaga gtcattcttg ttcagtggtg tttagactct gcatgatgat
     2341 atttgtttta tggattgaca aacaatgtct tggaggaatc agaaaatctt tttgtttaat
     2401 aaatgtttgc attattaagg tgatcataaa gcatgttgga cttctgaaaa catgtttgtg
     2461 tgtttatcgc catttaatat ccactgtttg tgcttcttaa tttaaatata tcagcatctt
     2521 atctgattat caattttttt gacccacaga taagtagtgg gggtgttttt tttagtagcc
     2581 tagatgcatg aatggaagca tctctgaatt tgtaacacct acctggtttg gcttttcaag
     2641 ggcttctgaa aactggcata tgagtgagca aaaacgcacc ctcttgccat tctgtggttt
     2701 gacccacagt tgactgttgt gctttcaatg ctaaacctgt cttactctta gaattaattg
     2761 gccagactgt ttattcatca agttttatga gcacatattt atacagcatt ttatttttat
     2821 ttgctgaaac aaattgtatg ctgattatct gcataatatc atatacattt taaatgcaaa
     2881 atgtgaccat ggaccacaaa actggttcta tgttacatgg gtacatttgt gggaatagct
     2941 aaaaatacat tgtataagtc aaatgataga ttatcattta ggcaaaaatg aattagaata
     3001 ttaagtaaag atcatcatcc atgaagacat tgttttaatt gtcatatcta tttaaacctt
     3061 atatcaatgg ataggtaact ttcaggtgat gtatacagta catttcaatt ttcaaaaata
     3121 taccctaatg tctggtcaca aatgaggatt tgttaaactt tgatagcagt tttgcaactg
     3181 gtgacttggt agactgagga tgatgctgtc attttcagaa cttgaacttt cagtccttca
     3241 aacactgctg tcatggaaaa agtttatgca ttttggccat ccgtttttgt ttacttttca
     3301 ttgccctcct ctcaccagat atatcacccc gcagtaactc ttaaaagtat gaggtgacct
     3361 taactcaaca aaagcctaat ttgtgagatg gattcgttct tgcagttgat ttagatgaga
     3421 tgatggcgga acagaacgtc cttgcaccat ttgtctgtcc tggcgaccag tgctgtcaat
     3481 cctgtcagct ctgagtgagg catgctgtaa tgaaatgaca caaaggttac gcttctcgaa
     3541 gttagagtta agttgaataa agttttgaaa agattaaccg ttttgagaca caatatccag
     3601 ttcaaagaat ttgaatattt agtgattctg ttttgccggt tagttccctc ttgtgtcagt
     3661 aaactgaata aatattgaac taattgcaaa tcattaaaac agttttgtgg gatttctgcc
     3721 gttaacctct ttgaaaatgg aggaatacaa aggaaaactt tagctgtgag tctgttgttt
     3781 tttttggttt atgtgaatgc tcagtgctca gtccgtctgg tcaggtctga aaggcatact
     3841 tcaacctttc tttttagccc tttaaaatac ctgtggacag atgggtcact ggaccagtta
     3901 agctgtttca gataggtatg gtttattcag tcacaaagcc aaaaggccat tcgcatccac
     3961 aggaaatacc tggctttact aatgggaggc ctccttttta tgttttgacg tacccttgta
     4021 aatgttatgg ccttccaata tctggtggtg agacttgtgg tggtttctga caatggttac
     4081 ggagttacac atggacgttt tgaagcattc aaagatctgc agcaaagcaa tgccatttta
     4141 ctaaagcact gagattttaa ccctcatttt ttgagattca tggcttaaag actaacatgt
     4201 tttatgttct aactaagtat ctattgcttt ctttttggat tttatgatgt aaaacattca
     4261 gtaccattct cttggattca aggtttaaca gtggctgacc attgtgggta ttaggcacta
     4321 gggatgcaca atatattgga atcaaattga ttattgacaa cagtacttgt gtgttgtatt
     4381 tgccaatatt aaatattaag ttttacatgc acatttcagc tgtttaacca cttagaaaac
     4441 tttttactct cactcagagt taatctcaaa aaagtttctc aaatcttgaa acgagtgtct
     4501 gtattttata ctgtatattc taatgtgaat tattttgtat aaatacaaaa tacgttttaa
     4561 aaaattagtt gtagattaaa aaataatcat ttgtaatgct taagacgtat gagatctggt
     4621 gcacaaagcc agctgaacaa actccggatt gcagattaag tttgagtaag cttaaaagac
     4681 taattaaagg tgcagttggt tattgcctta agaaatattt tttgttggtt gaaagtcttt
     4741 tcacgttcca atagtaatga ttaaagtaaa tgatctaaat gtatttatat atatttttat
     4801 attctgggta aggcataata ctgaaacatg ttcatccaat taaaacttgt tgggcagaca
     4861 attccactaa ttctgtaaat gtagatttgt acatctgtgc accccgttca cgcagatctg
     4921 ctgtttgaac gttagggctg tgggattaat cgaaacgaaa tcgtaaaggt aatcgtaaga
     4981 ggctgcagta taaaaaatat atatgaatat atgtaaacaa aaataacaaa taacatctga
     5041 ggtaaagtgc tgttaaatgc taaactatct gcaatggtgt cgtgaggcat cgcagacatg
     5101 gcatcagatg tcgtgaacta tgccacatta cactagaaga gacgattgaa tcttgtgtca
     5161 cgacgtttat ttccatttcc gacttcccac gacaccctac atcaaggaaa tagtgccaaa
     5221 atcttgtgat ctgaccgggg ctttataact aaacacactt tcattctgcc caatcaaaat
     5281 tgtgcaaccg aactgcccac aataaaaaaa aagaagaaaa aaaaacagga aagtgacaag
     5341 tgggattatg gcgtctgctg cttcataagc attaatagat gaattaatat taaagaaaaa
     5401 cagcatttga aatatgggaa tattttggtt tcaaactcac agacactaaa caaaaacagg
     5461 tcatttttaa gagctgtcac agaattgttg tcacagctta cagattattg agctgaagct
     5521 tgaattaaat tacaattagc ttgaagcttg aattaaatca taaatcaaat cgcagtcgcg
     5581 ataattataa atcatacact ttaaatcaat gattatcaac taaagagcaa tttgacttgg
     5641 caatgcttta aaagatgcta gtggactgtg agtggcttta ttgcaacaga tttatgtcac
     5701 tactcacaac tgattggtct aattttggtt tgcatggcga cgataccaga ttactcgact
     5761 tgaacctgaa aactcagttt gctctaacta aatgctatct ttcaaaaaaa atctaactgg
     5821 ctttgtgcaa cagacctatg acgacaattt aaaaataaaa ataaaagaaa taaatttttt
     5881 tttatatttt ggtagcaaaa atatatttta caattaaaat gattcgtttt agttctgaat
     5941 cattgagcag aaattataca gaatcgtaat atctatactt aattcttaac aagattctgt
     6001 actgggatca tcatatttgg ttgaaataaa ttctaagtag tccacatgat agctgtaatg
     6061 tcaaaaacac aagcaacgat ataatttaat ttgctttcag aaaagttatt ccagctaaaa
     6121 aacaattaaa acattgacag ccaatgagaa ttccaagttt gtacaaaaaa gttgaacgag
     6181 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     6241 cataatactg taaaacacaa catatgcagt cactatgaat caactactta gatggtatta
     6301 gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag
     6361 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     6421 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     6481 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     6541 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     6601 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     6661 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     6721 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     6781 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     6841 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     6901 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     6961 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     7021 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     7081 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     7141 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     7201 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     7261 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     7321 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     7381 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8101 cacatgtt
//
