LOCUS       40924_2902        3728 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-fMylz2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 3728)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3728)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      689..1773
                     /label=zebrafish fMylz2 promoter
                     /note="zebrafish fMylz2 promoter"
                     /regulatory_class="promoter"
     protein_bind    1773..1897
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1970..1988)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1993..2009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2122..2928
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3078..3666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgca aattagtcta aagaaagaag ggaatgcaca
      721 acaaactcaa gggggacaaa acagtgactg atgaattaga caagaaaaag agagcaagga
      781 gcgctcaaga ttgtttagct attttggtca cccacagctg ttccttatgc ctgccttccc
      841 aaaaaaaact gtcttaagcc tcaaatttct cttcatgagg gtccaacatc aaccactcag
      901 agggctgtag tgtgctgacc atctaaaaac tgggaaaaag gggtaattac gtgcttgtcc
      961 acagggcagc ttcccacaaa tggcacctca cagtcactga agtgaccggg tgaggtcata
     1021 ggtcgatcgg cagagagaga gagagagaga gagagagagt gctgaatatg ggcatggcca
     1081 tgtggacggg tgtggtgggg gcacttgaac cgaaatctta cagcatcact atactaggaa
     1141 aagcattaaa acctattttc gattagggct gatttgaaat aagggttaag acaccagaac
     1201 gtcctcttat atatcagcca ggggcgtgaa caaatatgaa caacataatc attggctcaa
     1261 aaaatctctg gattggaatc catcaggatc tatcactgca accctcccca tccaacaagt
     1321 aatcctgcaa gataagccag tattgatctg cagctaatct aactttaggg gagggagttg
     1381 cgtccccttt agactctgtg gctacagctc attcatttca aattgagtta tgtgattgta
     1441 tgaagctcaa accgtccctt acgtccccat gtccttatta gtcaacgcga gacatgcagg
     1501 ccgctgccat cagtatcaga ttcatcccat tccaagactc caatagctat ttctgagcac
     1561 tgtaagatga tagtacatcc cagccggtgt ccctccatca ctttccccct acctcatagt
     1621 tttgcctctt tctctctcgg tctgctattt cccaaacctc acttaaggtt gggtctataa
     1681 ttagcaaggg gccttcgtca gtatataagc ccctcaagta caggacacta cgcggcttca
     1741 gacttctctt cttgatcttc ttagacttca caccaagttt gtacaaaaaa gttgaacgag
     1801 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     1861 cataatactg taaaacacaa catatgcagt cactatgaat caactactta gatggtatta
     1921 gtgacctgta gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag
     1981 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     2041 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2101 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2161 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     2221 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     2281 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     2341 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     2401 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     2461 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     2521 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2581 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2641 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     2701 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2761 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2821 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2881 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     2941 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3001 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     3061 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     3481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3721 cacatgtt
//
