LOCUS       40924_2897        4511 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p5E-CAGGS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 4511)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4511)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (23-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /label=attL4
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        690..708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        741..1120
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1122..1399
                     /label=chicken beta-actin promoter
     intron          1400..2417
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     primer_bind     complement(2485..2501)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2538..2556)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2556..2680
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2753..2771)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2776..2792)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2905..3711
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3861..4449
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccgc gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggt aatacgactc actatagggc gaattgggta
      721 ccgggccccc cctcgaggtc gacattgatt attgactagt tattaatagt aatcaattac
      781 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      841 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      901 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      961 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1021 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1081 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     1141 ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt
     1201 ttaattattt tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc
     1261 ggggcggggc gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag
     1321 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     1381 gcgaagcgcg cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc
     1441 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     1501 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     1561 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     1621 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     1681 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     1741 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     1801 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     1861 gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc
     1921 ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt
     1981 gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg
     2041 gcggcccccg gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat
     2101 ggtaatcgtg cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat
     2161 ctgggaggcg ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca
     2221 ggaaggaaat gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc
     2281 tccagcctcg gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg
     2341 ggttcggctt ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct
     2401 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     2461 gcaaagaatt cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg
     2521 agctccagct tttgttccct ttagtgaggg ttaattcaag tttgtacaaa aaagttgaac
     2581 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     2641 ctacataata ctgtaaaaca caacatatgc agtcactatg aaccaactac ttagatggta
     2701 ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat cccctatagt
     2761 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2821 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2881 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2941 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     3001 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     3061 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     3121 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     3181 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     3241 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     3301 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     3361 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     3421 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     3481 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     3541 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     3601 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3661 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3721 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3781 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3841 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3901 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3961 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     4021 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     4081 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     4141 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4201 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     4261 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4321 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4381 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4441 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4501 gctcacatgt t
//
