LOCUS       V013005                 3601 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013005
VERSION     V013005
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        457..561
                     /label="AmpR promoter"
     CDS             562..1419
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1593..2181
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2469..2490
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2505..2535
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2543..2559
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2567..2583
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             join(2728..3601,1..5)
                     /gene="hisG"
                     /label="ATP phosphoribosyltransferase"
                     /note="ATP phosphoribosyltransferase from Salmonella
                     heidelberg (strain SL476). Accession#: B4T9N3"
ORIGIN
        1 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
       61 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      121 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
      181 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
      241 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
      301 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
      361 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      421 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      481 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      541 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      601 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      661 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      721 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      781 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      841 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      901 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      961 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1021 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1081 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1141 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1201 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1261 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1321 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1381 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1441 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1501 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1561 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2161 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2221 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     2281 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     2341 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2401 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2461 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2521 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     2581 gaccatgatt acgaattcga gctcggtacc ccagatcttc cagtggtgca tgaacgcatg
     2641 agaaagcccc cggaagatca tcttccgggg gctttttttt tggcgcgcga tacagaccgg
     2701 ttcagacagg ataaagagga acgcagaatg ttagacaaca cccgcttacg catagctatt
     2761 cagaaatcag gccgtttaag cgatgattca cgagaattgc tggcccgctg cggcataaaa
     2821 attaatttac acactcagcg cctgattgcg atggcggaaa acatgccgat tgatatcctg
     2881 cgcgtgcgtg atgatgacat tccgggtctg gtaatggatg gcgtggtcga tctcggtatt
     2941 atcggcgaaa acgtgctgga agaagagcta ctcaaccgcc gcgcacaggg cgaagatcca
     3001 cgctatttaa ccctgcgccg tcttgacttc ggcggctgcc gtttatcgct ggcaacaccg
     3061 gttgacgaag cctgggacgg cccggccgcg ctggacggta aacgtatcgc tacctcatat
     3121 ccgcacctcc tcaaacgcta cctcgaccag aaaggcgtct cttttaaatc gtgtctgtta
     3181 aatggttctg tcgaagtcgc gccgcgcgcg gggctggccg acgctatctg cgatttggtc
     3241 tctaccggcg cgacgcttga agctaacggc ctgcgtgaag tcgaagttat ctaccgctct
     3301 aaagcctgtc tgattcagcg cgacggtgag atggcacaga gcaagcaaga gctgatcgat
     3361 aaattgctga cccgtattca gggcgtgatt caggcgcgcg aatcgaaata catcatgatg
     3421 cacgcgccaa gtgaacgcct ggaagaggtt atcgccctgc tgccaggcgc cgaaaggccg
     3481 acaattctgc cgctggcagg cgagcaacag cgcgtggcga tgcacatggt cagcagcgaa
     3541 acgttgttct gggaaaccat ggagaaactg aaagcgcttg gcgccagctc gattctggta
     3601 c
//
