LOCUS       V005891                10730 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V005891
VERSION     V005891
KEYWORDS    p58sheLL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10730)
  AUTHORS   Kondo K, Ishii Y, Ochi H, Matsumoto T, Yoshikawa H, Kanda T
  TITLE     Neutralization of HPV16, 18, 31, and 58 pseudovirions with antisera
            induced by immunizing rabbits with synthetic peptides representing
            segments of the HPV16 minor capsid protein L2 surface region.
  JOURNAL   Virology. 2007 Feb 20;358(2):266-72. Epub 2006 Sep 28.
   PUBMED   17010405
REFERENCE   2  (bases 1 to 10730)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10730)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2007-02-20"; volume: "358(2)"; pages: "266-72. Epub 2006 Sep
            28"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..1513
                     /gene="L1"
                     /label="Major capsid protein L1"
                     /note="Major capsid protein L1 from Human papillomavirus
                     58. Accession#: P26535"
     CDS             2200..3615
                     /gene="L2"
                     /label="Minor capsid protein L2"
                     /note="Minor capsid protein L2 from Human papillomavirus
                     58. Accession#: P26538"
     misc_feature    3667..4255
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4298..4522
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4568..4996
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5005..5025)
                     /label="pBABE 3'"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6954..7319
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7452..7585
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7622..7638)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(7622..7638)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7635..7657)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    7646..7662
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7670..7700)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7715..7736)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7853..7870)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8024..8609)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8783..9640)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9641..9745)
                     /label="AmpR promoter"
     primer_bind     complement(9820..9839)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        10011..10390
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10391..10594
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    10596..10614
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10617..10635
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10645..10669
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccggagggc cgcgccacca tgagcgtgtg gaggcccagc gaggccaccg tgtacctgcc
       61 ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtgagcagga ccagcatcta
      121 ctactacgcc ggcagcagca ggctgctggc cgtgggcaac ccctacttca gcatcaagag
      181 ccccaacaac aacaagaagg tgctggtgcc caaggtgagc ggcctgcagt acagggtgtt
      241 cagggtgagg ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc
      301 cgacacccag aggctggtgt gggcctgcgt gggcctggag atcggcaggg gccagcccct
      361 gggcgtgggc gtgagcggcc acccctacct gaacaagttc gacgacaccg agaccagcaa
      421 caggtacccc gcccagcccg gcagcgacaa cagggagtgc ctgagcatgg actacaagca
      481 gacccagctg tgcctgatcg gctgcaagcc ccccaccggc gagcactggg gcaagggcgt
      541 ggcctgcaac aacaacgccg ccgccaccga ctgccccccc ctggagctgt tcaacagcat
      601 catcgaggac ggcgacatgg tggacaccgg cttcggctgc atggacttcg gcaccctgca
      661 ggccaacaag agcgacgtgc ccatcgacat ctgcaacagc acctgcaagt accccgacta
      721 cctgaagatg gccagcgagc cctacggcga cagcctgttc ttcttcctga ggagggagca
      781 gatgttcgtg aggcacttct tcaacagggc cggcaagctg ggcgaggccg tgcccgacga
      841 cctgtacatc aagggcagcg gcaacaccgc cgtgatccag agcagcgcct tcttccccac
      901 ccccagcggc agcatcgtga ccagcgagag ccagctgttc aacaagccct actggctgca
      961 gagggcccag ggccacaaca acggcatctg ctggggcaac cagctgttcg tgaccgtggt
     1021 ggacaccacc aggagcacca acatgaccct gtgcaccgag gtgaccaagg agggcaccta
     1081 caagaacgac aacttcaagg agtacgtgag gcacgtggag gagtacgacc tgcagttcgt
     1141 gttccagctg tgcaagatca ccctgaccgc cgagatcatg acctacatcc acaccatgga
     1201 cagcaacatc ctggaggact ggcagttcgg cctgaccccc ccccccagcg ccagcctgca
     1261 ggacacctac aggttcgtga ccagccaggc catcacctgc cagaagaccg ccccccccaa
     1321 ggagaaggag gaccccctga acaagtacac cttctgggag gtgaacctga aggagaagtt
     1381 cagcgccgac ctggaccagt tccccctggg caggaagttc ctgctgcaga gcggcctgaa
     1441 ggccaagccc aggctgaaga ggagcgcccc caccaccagg gcccccagca ccaagaggaa
     1501 gaaggtgaag aagtgaaagc tttttgaatt ctttggatcc actagggctc gaggaagctt
     1561 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
     1621 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
     1681 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
     1741 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
     1801 ttcgagaagc ccagtaccac ctcggaatct tcgatgcgtt gcgctcagca ctcaacccca
     1861 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
     1921 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
     1981 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
     2041 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
     2101 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
     2161 ttgttatcat aaagcgaatt ggattgcggc cgcgccacca tgaggcacaa gaggagcacc
     2221 aggaggaaga gggccagcgc cacccagctg taccagacct gcaaggccag cggcacctgc
     2281 ccccccgacg tgatccccaa ggtggagggc accaccatcg ccgaccagat cctgaggtac
     2341 ggcagcctgg gcgtgttctt cggcggcctg ggcatcggca ccggcagcgg caccggcggc
     2401 aggaccggct acgtgcccct gggcagcacc ccccccagcg aggccatccc cctgcagccc
     2461 atcaggcccc ccgtgaccgt ggacaccgtg ggccccctgg acagcagcat cgtgagcctg
     2521 atcgaggaga gcagcttcat cgacgccggc gcccccgccc ccagcatccc cacccccagc
     2581 ggcttcgaca tcaccaccag cgccgacacc acccccgcca tcctgaacgt gagcagcatc
     2641 ggcgagagca gcatccagac cgtgagcacc cacctgaacc ccagcttcac cgagcccagc
     2701 gtgctgaggc cccccgcccc cgccgaggcc agcggccacc tgatcttcag cagccccacc
     2761 gtgagcaccc acagctacga gaacatcccc atggacacct tcgtgatcag caccgacagc
     2821 ggcaacgtga ccagcagcac ccccatcccc ggcagcaggc ccgtggccag gctgggcctg
     2881 tacagcagga acacccagca ggtgaaggtg gtggaccccg ccttcctgac cagcccccac
     2941 aggctggtga cctacgacaa ccccgccttc gagggcttca accccgagga caccctgcag
     3001 ttccagcaca gcgacatcag ccccgccccc gaccccgact tcctggacat cgtggccctg
     3061 cacaggcccg ccctgaccag caggaggggc accgtgaggt acagcagggt gggccagaag
     3121 gccaccctga ggaccaggag cggcaagcag atcggcgcca aggtgcacta ctaccaggac
     3181 ctgagcccca tccagcccgt gcaggagcag gtgcagcagc agcagcagtt cgagctgcag
     3241 agcctgaaca ccagcgtgag cccctacagc atcaacgacg gcctgtacga catctacgcc
     3301 gacgacgccg acaccatcca cgacttccag agccccctgc acagccacac cagcttcgcc
     3361 accaccagga ccagcaacgt gagcatcccc ctgaacaccg gcttcgacac ccccctggtg
     3421 agcctggagc ccggccccga catcgccagc agcgtgacca gcatgagcag ccccttcatc
     3481 cccatcagcc ccctgacccc cttcaacacc atcatcgtgg acggcgccga cttcatgctg
     3541 caccccagct acttcatcct gaggaggagg aggaagaggt tcccctactt cttcgccgac
     3601 gtgagggtgg ccgcctgaaa gctttttgaa ttctttggat ccactagcga tatcaagctt
     3661 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3721 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3781 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     3841 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     3901 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     3961 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4021 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4081 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4141 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4201 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     4261 taccgtcggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     4321 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     4381 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     4441 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     4501 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     4561 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     4621 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     4681 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     4741 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     4801 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     4861 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4921 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4981 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5041 gctccccagc aggcagaagt atgcaaagca tgcagaattc tatcaaatat ttaaagaaaa
     5101 aaaaattgta tcaactttct acaatctctt tcagaagaca gaagcagagg gaatacttcc
     5161 taaatcattc aactaggcca gcattacctt aataccggaa ctagaaaatg acattacaag
     5221 aaaagaaaac aacagaccaa tatctctcat gaacaaagat acaaacattt tcaacaaaat
     5281 attagcaaaa agaatccaag aatgtatcaa aaaatataca ccacaaccaa gtagaattta
     5341 ttccagatat gtaagggtgg ttcaacgttt gaaaatcaat taacgtaatt tgtcccatca
     5401 acaggttaaa gaagaaaatc acatggtcat attgatagac acagaaaaag catttgacaa
     5461 aatttaacac ccattcatga tgcaatctct cagtaaacta ggaatagagg aaaacttcct
     5521 cagcttgaat gtaccttcct ctcaattttg ctatgaacct gaaactcctc ttaaaaaata
     5581 aagtttttca tttaaaaaga aaacaaaaaa catggaggag cgttgatgta tctcatttta
     5641 gaccaatcag ctatggatag ttaggcgaca gcacagatag ctgctgtact tctgtttctg
     5701 gcaatgttcc agactacatt taaaaaattt ttaattatag acttgtactt aatgttcaag
     5761 aaaaatatga aaatggcttt gccgtgttaa tgctactctt ttttaaaaaa aactaaagtt
     5821 caaactttat ttatatttca ttagtttttt agctactgtt ctttttctgt tctgggatct
     5881 cattcagaat gccacattac atataattct catgtctcct tgggttcctc ttagttttga
     5941 cagttcctca gacttttctt atttttgatg accttgacag ttttgaggag tactggttag
     6001 atatagggta atggttttta aagtatattt gtcatgattt atactggggt aagggtttgg
     6061 ggaggaagcc catggggtaa agtactgttc tcatcacatc atatcaaggt tatataccat
     6121 caatattgcc acagatgtta cttagccttt taatatttct ctaatttagt gtatatgcaa
     6181 tgatagttct ctgatttctg agattgagtt tctcatgtgt aatgattatt tagagtttct
     6241 ctttcatctg ttcaaatttt tgtctagttt tattttttac tgatttgtaa gacttctttt
     6301 tataatctgc atattacaat tctctttact ggggtgttgc aaatattttc tgtcattcta
     6361 tggcctgact tttcttaatg gttttttaat tttaaaaata agtcttaata ttcatgcaat
     6421 ctaattaaca atcttttctt tgtggttagg actttgagtc ataagaaatt tttctctaca
     6481 ctgaagtcat gatggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
     6541 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
     6601 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagaaa aagatcatca
     6661 ttttcccatt gtaaaatgcc atattttttt cataggtcac ttacatatat caatgggtct
     6721 gtttctgagc tctactctat tttatcagcc tcactgtcta tccccacaca tctcatgctt
     6781 tgctctaaat cttgatattt agtggaacat tctttcccat tttgttctac aagaatattt
     6841 ttgttattgt cttttgggct tctatataca ttttagaatg aggttggcaa gttaacaaac
     6901 agcttttttg gggtgaacat attgactaca aatttatgtg gaaagaaagt accaagttga
     6961 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     7021 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     7081 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     7141 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     7201 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     7261 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     7321 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     7381 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     7441 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7561 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7621 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7681 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7741 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7801 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7861 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7921 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7981 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8041 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8101 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     8161 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     8221 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     8281 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8341 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8401 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8461 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8521 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     8581 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8641 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8701 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     9001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     9061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     9121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     9181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9721 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9781 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9841 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
     9901 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
     9961 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
    10021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
    10081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
    10141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
    10201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
    10261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
    10321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
    10381 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
    10441 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggaaccaaaa
    10501 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
    10561 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
    10621 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
    10681 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
//
