LOCUS       62056_2200        4695 bp DNA     circular SYN 25-SEP-2019
DEFINITION  Cloning vector p57-V5+NeoR, complete sequence.
ACCESSION   MN047315
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Kachale A, Kraeva N, Bianchi C, Yurchenko V, Lukes J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2019) Laboratory of Molecular Biology of Protists,
            Biology Centre CAS, Institute of Parasitology, Branisovska 31, Ceske
            Budejovice, Sb 37005, Czech Republic
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-JUN-2019) Laboratory of Molecular Biology of Protists, Biology 
            Centre CAS, Institute of Parasitology, Branisovska 31, Ceske 
            Budejovice, Sb 37005, Czech Republic"
FEATURES             Location/Qualifiers
     source          1..4695
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           663..1082
                     /note="5' noncoding region of hexokinase gene (420bp part)
                     from Blastocrithidia sp. p57"
     CDS             1086..1127
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             1221..2018
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     3'UTR           2022..2441
                     /note="3' noncoding region of hexokinase gene (420bp part)
                     from Blastocrithidia sp. p57"
     primer_bind     complement(2454..2470)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2507..2525)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2546..2562)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2570..2586)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2594..2624)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2639..2660)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2948..3536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3710..4567)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4568..4672)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgctatttaa
      661 atattgccca tgtccggtgc tcatgcgtta ttgtcgaacg atcgatgaaa ggcgaaaaag
      721 ttatttatac tgcagcgatc tacaataaat ttgaataaat acagaacaac taaagttccg
      781 gatgtactaa tacattagac acttatacaa ataatataat taaataatct ggaaaaaaat
      841 aattatcaaa gccataaaaa atgatgaact tcccatatac aaatattcaa atttataaca
      901 ataatttagc tcatatatca catgtaaaaa taactttatt aatgtttagt ctatgttatt
      961 ttaatttatt attctcatat aattatattt ttctcaattt aacaagcaat ctaagcaact
     1021 atcattcaga taattcaata caattttatc tattataaaa caaaataaat tttggagtaa
     1081 taatgggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcga ggtaagccaa
     1141 tcccaaaccc acttcttggt ctcgactcaa cacagctggg taagcccatc cccaaccccc
     1201 tcctcggtct tgatagcacg ggatcggcca ttgaacaaga tggattgcac gcaggttctc
     1261 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     1321 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     1381 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     1441 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     1501 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     1561 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     1621 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     1681 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     1741 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacgcat ggcgatgcct
     1801 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     1861 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     1921 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     1981 agcgcatcgc cttctatcgc cttcttgacg agttcttcta aagttgcagt taaggtcata
     2041 ttctttttga ccagttaaag ttaaatgttt gcattaaaca ccaaaagtaa ataactaatt
     2101 acttttactc cctctttctc ttgataacat ttctttgaac ataaaacaca tagcttatca
     2161 tattttcttt gaaaagttta ataaacaaat ttaaactttc ttttccctat gtattgctta
     2221 ataattcaat aacactttct tctatacctg taaatctgca taaagaagtt attttagagt
     2281 aatgtacttt tgatcaattc gacacgtaca aaatttatta agcttaacta aaaataatct
     2341 ccgttttagc aataatagaa gtatatatcc ttccacctta tatttttgca cattattaaa
     2401 acggtgcaat tatttatttt ataaatgatc tggaagtatt tatttaaatc taggatccac
     2461 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     2521 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2581 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2641 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2701 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2761 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2821 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2881 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2941 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3001 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3061 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3121 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3181 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3241 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3301 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3361 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3421 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3481 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3541 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3601 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3661 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3721 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3781 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3841 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3901 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3961 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4021 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4081 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4141 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4201 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4261 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4321 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4381 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4441 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4501 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4561 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4621 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4681 cccgaaaagt gccac
//
