LOCUS       40924_2882        5909 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p53-luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Zheng C-F., Grafsky A.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-1998) Technical Services, Stratagene, 11011 N. 
            Torrey PInes Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Zheng C-F., Grafsky A.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-DEC-1998) Technical Services, Stratagene, 11011 N. 
            Torrey PInes Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 5909)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-1998) Technical Services, Stratagene, 11011 N. Torrey PInes 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-DEC-1998) Technical Services, Stratagene, 11011 N. Torrey PInes 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     On Dec 30, 1998 this sequence version replaced gi:3548971.
FEATURES             Location/Qualifiers
     source          1..5909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        32..136
                     /label=AmpR promoter
     CDS             137..994
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1168..1755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1934..2074)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     polyA_signal    2313..2447
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2541..2673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             3030..4679
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     intron          4813..4878
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5448..5582
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     RBS             5617..5625
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
       61 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      121 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      181 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      241 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      301 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      361 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      421 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      481 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      541 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      601 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      661 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      721 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      781 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      841 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      901 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      961 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1021 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1081 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1141 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1201 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1261 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     1321 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1381 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1441 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1501 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca
     1561 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1621 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1681 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1741 gcggagctat ggaaaaacgc cagcaacgcg cctttttacg gttcctggcc ttttgctggc
     1801 cttttgctca catgttcttt cctgcgttat ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtgc tgataccgct cgccgcagcc gaacgaccga gcgcaagtca gtgagcgagg
     1921 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     1981 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     2041 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     2101 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     2161 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcaggatca
     2221 gccatatcac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2281 acctgaaaca taaaatgaat gcaattgttg ttaacttgtt tattgcagct tataatggtt
     2341 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2401 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcagc catatcacat
     2461 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2521 aaatgaatgc aattgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     2581 atagcatcac aaatttcaca aataaagcaa tttttcactg cattctattg tggtttgtcc
     2641 aaactcatca atgtatctta tcatgtctgg atccaagctc ctgcctggac ttgcctggcc
     2701 tgcctggact tgcctggcct gcctggactt gcctgcctgg acttgcctgg cctgcctgga
     2761 cttgcctggc ctgcctggac ttgcctggcc tgcctggact tgcctggcct gcctggactt
     2821 gcctggcctg cctggacttg cctggcctgc ctggacttgc ctggcctgcc tggacttgcc
     2881 tggcctgcct ggacttgcct ggcctgcctg gacttgcctg gcctgcctgg acttgcctgg
     2941 cctgcctgga cttgcctggg cggaggactc tagaggggta tataatggaa gctcgaattc
     3001 cagcttggca ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc
     3061 cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat aaggctatga
     3121 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtgaaca
     3181 tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
     3241 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
     3301 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
     3361 aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt gtttccaaaa
     3421 aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag aaaattatta
     3481 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
     3541 atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa
     3601 caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt gtggcccttc
     3661 cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt ggcaatcaaa
     3721 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
     3781 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
     3841 agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta gtaccaaccc
     3901 tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
     3961 aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct
     4021 tccatcttcc agggatacga caaggatatg ggctcactga gactacatca gctattctga
     4081 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     4141 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga ggcgaattat
     4201 gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     4261 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     4321 acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat caggtggccc
     4381 ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg ggcgtggcag
     4441 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     4501 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     4561 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     4621 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag tccaaattgt
     4681 aaaatgtaac tgtattcagc gatgacgaaa ttcttagcta ttgtaatact ctagaggatc
     4741 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     4801 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     4861 attgtttgtg tattttagat tccaacctat ggaacttatg aatgggagca gtggtggaat
     4921 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     4981 actgctgact ctcaacattc tactctcaaa agaagagaaa ggtagagacc caaggacttt
     5041 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     5101 tttgctattt acaaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     5161 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     5221 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     5281 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     5341 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5401 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5461 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5521 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5581 tccccaggaa gctcctctgt gtcctcataa accctaacct ccttacttga gaggacattc
     5641 caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
     5701 aggaaattgg gtaggggttt ttcacagacc gctttctaag gggtaatttt aaaatatctg
     5761 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     5821 gcagaaacat acaagctgtc actttgcaca aagggcctcg tgatacgcct atttttatag
     5881 gttaatgtca tgataataat ggtttctta
//
