LOCUS       62056_2195        7421 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    168..189
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        204..234
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    242..258
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     266..282
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        303..321
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(406..1077)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(1078..1359)
                     /label=TRP1 promoter
     CDS             complement(3127..3786)
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(3982..4000)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4007..4023)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4164..4618
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    4935..5438
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5475..5579
                     /label=AmpR promoter
     CDS             5580..6371
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      6545..7133
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tctggtgtcg tcgcgcagcc gacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
       61 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      121 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      181 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      241 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
      301 gaaattaacc ctcactaaag ggaacaaaag cttgcatgcc tgcaggtcga ctctagggca
      361 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt
      421 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac
      481 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc
      541 accagctaac ataaaatgta agctttcggg gctctcttgc cttccaaccc agtcagaaat
      601 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa
      661 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag
      721 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg
      781 cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg
      841 attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac
      901 aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca
      961 tataataccc agcaagtcag catcggaatc tagagcacat tctgcggcct ctgtgctctg
     1021 caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact
     1081 ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc
     1141 tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttaatc ggcaaaaaaa
     1201 gaaaagctcc ggatcaagat tgtacgtaag gtgacaagct atttttcaat aaagaatatc
     1261 ttccactact gccatctggc gtcataactg caaagtacac atatattacg atgctgttct
     1321 attaaatgct tcctatatta tatatatagt aatgtcgtta aaaaaatttc aaggaaaccg
     1381 aaatcaaaaa aaagaataaa aaaaaaatga tgaaattgaa accccccccc ccccccccga
     1441 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     1501 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     1561 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     1621 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     1681 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt
     1741 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     1801 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     1861 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     1921 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     1981 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     2041 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     2101 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     2161 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
     2221 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     2281 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     2341 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     2401 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     2461 ggccacgatg cgtccggcgt agaggatcct cggggacacc aaatatggcg atctcggcct
     2521 tttcgtttct tggagctggg acatgtttgc catcgatcca tctaccacca gaacggccgt
     2581 tagatctgct gccaccgttg tttccaccga agaaaccacc gttgccgtaa ccaccacgac
     2641 ggttgttgct aaagaagctg ccaccgccac ggccaccgtt gtagccgccg ttgttgttat
     2701 tgtagttgct actgttattt ctggcacttc ttggttttcc tcttaagtga ggaggaacat
     2761 aaccattctc gttgttgtcg ttgatgctta aattttgcac ttgttcgctc agttcagcca
     2821 taatatgaaa tgcttttctt gttgttctta cggaatacca cttgccacct atcaccacaa
     2881 ctaacttttt cccgttcctc catctctttt atattttttt tctcgagttc aagagaaaaa
     2941 aaaagaaaaa gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa
     3001 tgatgcatta ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc
     3061 ataggtatac atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg
     3121 tcactacata agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc
     3181 ttctcttatg gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt
     3241 gcctcgcaga caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa
     3301 atgcgggatc atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac
     3361 aactgcgtac ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg
     3421 cgcaaatcct gatccaaacc tttttactcc acgcacggcc cctagggcct ctttaaaagc
     3481 ttgaccgaga gcaatcccgc agtcttcagt ggtgtgatgg tcgtctatgt gtaagtcacc
     3541 aatgcactca acgattagcg accagccgga atgcttggcc agagcatgta tcatatggtc
     3601 cagaaaccct atacctgtgt ggacgttaat cacttgcgat tgtgtggcct gttctgctac
     3661 tgcttctgcc tctttttctg ggaagatcga gtgctctatc gctaggggac caccctttaa
     3721 agagatcgca atctgaatct tggtttcatt tgtaatacgc tttactaggg ctttctgctc
     3781 tgtcatcttt gccttcgttt atcttgcctg ctcatttttt agtatattct tcgaagaaat
     3841 cacattactt tatataatgt ataattcatt atgtgataat gccaggaatt tctagaggat
     3901 ccaggcatgc ctaggcatgc ctaggcatgc ctaggcatgc ctaggcatgc taggcatgcc
     3961 tggtaccgag ctcgaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     4021 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4081 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4141 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4501 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4561 tgagctgatt taacaaaaat ttaacgcgaa tttaacaaaa tattaacgct tacaatttcc
     4621 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     4681 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4741 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4801 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4921 cgtcggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa
     4981 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
     5041 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc
     5101 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
     5161 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
     5221 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
     5281 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
     5341 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
     5401 aaagatacga ggcgcgtgta agttacaggc aagcgatccg tccgacgtca ggtggcactt
     5461 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5521 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5581 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5641 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5701 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     5761 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5821 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5881 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5941 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6001 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6061 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     6121 gcgaggatct cgtcgtgact catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6181 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6241 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6301 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6361 acgagttctt ctgactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6421 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6481 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6541 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6601 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6661 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     6721 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6781 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6841 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6901 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6961 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7021 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7081 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7141 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7201 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7261 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7321 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7381 ggtttcccga ctggaaagcg ggcaggagcg cacgcgatcc t
//
