LOCUS       40924_2862        4919 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Reporter vector p50huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      88..137
                     /label=hIL6 promoter fragment
                     /note="hIL6 promoter fragment"
                     /regulatory_class="promoter"
     CDS             185..1834
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1878..1999)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2422..3010)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3184..4041)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4042..4146)
                     /label=AmpR promoter
     rep_origin      4173..4628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4759..4807
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgc
       61 atgcctgcag gtcgactcta gaggatcatt agagtctcaa cccccaataa atataggact
      121 ggagatgtct gaggctcatt ctgccctcga agcttggcat tccggtactg ttggtaaagc
      181 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
      241 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      301 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
      361 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      421 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      481 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
      541 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      601 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca
      661 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata
      721 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc
      781 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt
      841 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag
      901 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg
      961 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga
     1021 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac
     1081 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct
     1141 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg
     1201 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc
     1261 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg
     1321 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc
     1381 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca
     1441 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa
     1501 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct
     1561 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga
     1621 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt
     1681 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt
     1741 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct
     1801 cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc
     1861 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     1921 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     1981 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2041 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa
     2101 ggatccgtcg atcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2161 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2221 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2281 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2341 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2401 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2461 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2521 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2581 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2641 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2701 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2761 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2821 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2881 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2941 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3001 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3061 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3121 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3181 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3241 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3301 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3361 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3421 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3481 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3541 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3601 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3661 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3721 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3781 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3841 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3901 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3961 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4021 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4081 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4141 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     4201 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4261 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4321 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4381 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4441 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4501 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4561 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4621 tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4681 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga
     4741 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4801 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4861 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//
