LOCUS       40924_2842        4330 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p4g3R, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed emergence of a domain of gene expression in a bacterial 
            population
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Boehm CR.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 4330)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-OCT-2016) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      57..88
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      213..307
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      799..1021
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1069..2016
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     regulatory      2229..2274
                     /label=PT7(-3G)
                     /note="PT7(-3G)"
                     /regulatory_class="promoter"
     regulatory      2283..2294
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             2283..2294
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2301..2975
                     /label=mRFP1
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     terminator      2998..3069
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3085..3112
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3119..3139
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             3243..4100
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      4114..4173
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(4233..4262)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4309..4330
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagctgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac
      241 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
      301 tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt
      361 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
      421 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
      481 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
      541 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg
      601 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
      661 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
      721 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc
      781 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt
      841 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa
      901 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag
      961 cagaatttac agatacccac aactcaaagg aaaaggacat gtaattatca ttgactagcc
     1021 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta
     1081 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc
     1141 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa
     1201 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa
     1261 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc
     1321 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca
     1381 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa
     1441 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg
     1501 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag
     1561 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa
     1621 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg
     1681 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta
     1741 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac
     1801 aaaataccaa caaccattac atcagattcc tacctacata acggactaag aaaaacacta
     1861 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac
     1921 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc
     1981 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc
     2041 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc
     2101 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga
     2161 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac
     2221 tactagagta atacgactca ctgtagggaa tacaagctac ttgttctttt tgcatactag
     2281 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
     2341 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
     2401 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
     2461 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     2521 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     2581 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
     2641 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     2701 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     2761 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     2821 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     2881 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     2941 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata atactagagc caggcatcaa
     3001 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3061 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     3121 ctagtagcgg ccgctgcagt gatctacact agcactatca gtgctaaata cattcaaata
     3181 tctatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3241 atatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     3301 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgccgaagat cagttgggtg
     3361 cacgtgtggg ttacatcgaa ctggacctca acagcggtaa gattcttgag agttttcgcc
     3421 ccgaagaacg tttcccaatg atgagcactt ttaaagttct gctctgtggc gcggtattat
     3481 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     3541 tggttgagta ctcaccagtc acagaaaagc atcttacgga cggcatgaca gtacgcgaat
     3601 tatgcagcgc tgccataacc atgagtgata acacggcggc caacttactt ctgacaacga
     3661 tcggaggacc gaaggagctt accgcttttt tgcacaacat gggtgatcat gtaactcgcc
     3721 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3781 tgcctgtagc tatggcaaca acgttgcgca aactcttaac tggcgaactt cttactctcg
     3841 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3901 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3961 cccgcggtat tattgcagcc ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4021 acacgacggg gagccaggca actatggacg aacgtaatcg ccagatcgct gagataggtg
     4081 cctccctgat taagcattgg taataatact agctccggca aaaaaacggg caaggtgtca
     4141 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgtttgcca cctgacgtct
     4201 aagaaaagga atattcagca atttgcccgt gccgaagaaa ggcccacccg tgaaggtgag
     4261 ccagtgagtt gattgctacg taattagtta gttagccctt agtgactcga attcgcggcc
     4321 gcttctagag
//
