LOCUS       40924_2817       10900 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector p4305.1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Noon JB, Maier TR, Baum TJ.
  TITLE     HgGLAND18 is a malaria-like effector of the soybean cyst nematode 
            that is important for virulence on soybean and efficiently targets 
            host cell nucleoli for suppression of innate immunity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Noon JB, Maier TR, Baum TJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2015) Plant Pathology and Microbiology, Iowa State
            University, 351 Bessey Hall, Ames, IA 50011, USA
REFERENCE   3  (bases 1 to 10900)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10900)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2015) Plant Pathology and Microbiology, Iowa State 
            University, 351 Bessey Hall, Ames, IA 50011, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..27)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(35..51)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(59..89)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(104..125)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        316..993
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             1035..1751
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     polyA_signal    1761..1935
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2013..2037)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2462..3253
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3343..3931
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4117..4257)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4601..4795)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4864..5928)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(6365..6991)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(8292..8316)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8519..8535
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8539..8584
                     /label=multiple cloning site (MCS)
                     /note="multiple cloning site (MCS)"
     intron          8589..8778
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             8773..10596
                     /codon_start=1
                     /label=GUSPlus(TM)
                     /note="beta-glucuronidase"
                     /translation="LQNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYESKLT
                     DTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAIVYV
                     NGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGLGKV
                     IRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAETVK
                     VSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEPFGV
                     RTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFRTAH
                     YPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHHQDV
                     LRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLFVMA
                     TPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITEYGA
                     DTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRVQGN
                     KKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     CDS             10603..10620
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      10641..10893
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
       61 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      121 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      181 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag agcagcttgc
      241 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
      301 agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
      361 ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta
      421 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg
      481 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
      541 gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacactc tcgtctactc
      601 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
      661 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
      721 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
      781 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
      841 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
      901 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccttc ctctatataa
      961 ggaagttcat ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta
     1021 tctctctcga gacaatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1081 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1141 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1201 tgccctggcc caccctcgtg accaccttca cctacggcgt gcagtgcttc agccgctacc
     1261 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1321 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1381 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1441 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1501 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1561 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1621 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1681 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactcac ggcatggacg
     1741 agctgtacaa gtaactcgag tttctccata ataatgtgtg agtagttccc agataaggga
     1801 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     1861 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     1921 actaaaatcc agatcccccg aattaattcg gcgttaattc agtacattaa aaacgtccgc
     1981 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     2041 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     2101 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     2161 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     2221 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg
     2281 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     2341 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     2401 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     2461 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     2521 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     2581 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     2641 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     2701 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     2761 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     2821 gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt
     2881 agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga
     2941 agaagacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc
     3001 cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga
     3061 tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga
     3121 cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct
     3181 attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact
     3241 ggatgaattg ttttagtacc tagaatgcat gaccaaaatc ccttaacgtg agttttcgtt
     3301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3481 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3961 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4021 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4081 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     4141 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     4201 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     4261 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4321 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4381 ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc
     4441 ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc
     4501 ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg
     4561 cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt
     4621 tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt
     4681 tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta
     4741 cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa
     4801 gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca
     4861 ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg
     4921 gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag
     4981 cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat
     5041 cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag
     5101 aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg
     5161 gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc
     5221 cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga
     5281 taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt
     5341 gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg
     5401 gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt
     5461 cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc
     5521 gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg
     5581 aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc
     5641 cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa
     5701 atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg
     5761 ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc
     5821 ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc
     5881 atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgt cacgccgatt
     5941 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
     6001 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
     6061 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
     6121 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
     6181 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
     6241 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
     6301 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
     6361 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
     6421 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
     6481 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
     6541 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
     6601 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
     6661 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
     6721 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
     6781 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
     6841 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
     6901 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
     6961 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
     7021 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
     7081 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
     7141 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
     7201 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
     7261 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
     7321 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
     7381 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
     7441 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
     7501 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
     7561 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
     7621 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
     7681 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
     7741 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
     7801 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
     7861 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
     7921 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
     7981 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
     8041 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
     8101 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
     8161 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
     8221 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
     8281 ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg
     8341 cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt caggctgcgc
     8401 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     8461 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     8521 aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg actctagatg accatggtag
     8581 atctgagggt aaatttctag tttttctcct tcattttctt ggttaggacc cttttctctt
     8641 tttatttttt tgagctttga tctttcttta aactgatcta ttttttaatt gattggttat
     8701 ggtgtaaata ttacatagct ttaactgata atctgattac tttatttcgt gtgtctatga
     8761 tgatgatgat agttacagaa ccgacgaact agtctgtacc cgatcaacac cgagacccgt
     8821 ggcgtcttcg acctcaatgg cgtctggaac ttcaagctgg actacgggaa aggactggaa
     8881 gagaagtggt acgaaagcaa gctgaccgac actattagta tggccgtccc aagcagttac
     8941 aatgacattg gcgtgaccaa ggaaatccgc aaccatatcg gatatgtctg gtacgaacgt
     9001 gagttcacgg tgccggccta tctgaaggat cagcgtatcg tgctccgctt cggctctgca
     9061 actcacaaag caattgtcta tgtcaatggt gagctggtcg tggagcacaa gggcggattc
     9121 ctgccattcg aagcggaaat caacaactcg ctgcgtgatg gcatgaatcg cgtcaccgtc
     9181 gccgtggaca acatcctcga cgatagcacc ctcccggtgg ggctgtacag cgagcgccac
     9241 gaagagggcc tcggaaaagt cattcgtaac aagccgaact tcgacttctt caactatgca
     9301 ggcctgcacc gtccggtgaa aatctacacg accccgttta cgtacgtcga ggacatctcg
     9361 gttgtgaccg acttcaatgg cccaaccggg actgtgacct atacggtgga ctttcaaggc
     9421 aaagccgaga ccgtgaaagt gtcggtcgtg gatgaggaag gcaaagtggt cgcaagcacc
     9481 gagggcctga gcggtaacgt ggagattccg aatgtcatcc tctgggaacc actgaacacg
     9541 tatctctacc agatcaaagt ggaactggtg aacgacggac tgaccatcga tgtctatgaa
     9601 gagccgttcg gcgtgcggac cgtggaagtc aacgacggca agttcctcat caacaacaaa
     9661 ccgttctact tcaagggctt tggcaaacat gaggacactc ctatcaacgg ccgtggcttt
     9721 aacgaagcga gcaatgtgat ggatttcaat atcctcaaat ggatcggcgc caacagcttc
     9781 cggaccgcac actatccgta ctctgaagag ttgatgcgtc ttgcggatcg cgagggtctg
     9841 gtcgtgatcg acgagactcc ggcagttggc gtgcacctca acttcatggc caccacggga
     9901 ctcggcgaag gcagcgagcg cgtcagtacc tgggagaaga ttcggacgtt tgagcaccat
     9961 caagacgttc tccgtgaact ggtgtctcgt gacaagaacc atccaagcgt cgtgatgtgg
    10021 agcatcgcca acgaggcggc gactgaggaa gagggcgcgt acgagtactt caagccgttg
    10081 gtggagctga ccaaggaact cgacccacag aagcgtccgg tcacgatcgt gctgtttgtg
    10141 atggctaccc cggagacgga caaagtcgcc gaactgattg acgtcatcgc gctcaatcgc
    10201 tataacggat ggtacttcga tggcggtgat ctcgaagcgg ccaaagtcca tctccgccag
    10261 gaatttcacg cgtggaacaa gcgttgccca ggaaagccga tcatgatcac tgagtacggc
    10321 gcagacaccg ttgcgggctt tcacgacatt gatccagtga tgttcaccga ggaatatcaa
    10381 gtcgagtact accaggcgaa ccacgtcgtg ttcgatgagt ttgagaactt cgtgggtgag
    10441 caagcgtgga acttcgcgga cttcgcgacc tctcagggcg tgatgcgcgt ccaaggaaac
    10501 aagaagggcg tgttcactcg tgaccgcaag ccgaagctcg ccgcgcacgt ctttcgcgag
    10561 cgctggacca acattccaga tttcggctac aagaacgcta gccatcacca tcaccatcac
    10621 gtgtgagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
    10681 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
    10741 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
    10801 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
    10861 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
//
