LOCUS       40924_2762        6934 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector p413-TR-SSRE-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Artificial cell-cell communication in yeast Saccharomyces cerevisiae
            using signaling elements from Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Electrical Engineering, Princeton 
            University, Engineering Quadrangle, Olden Street, Princeton, NJ 
            08544, USA
REFERENCE   3  (bases 1 to 6934)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2005) Electrical Engineering, Princeton University, 
            Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        317..503
                     /label=HIS3 promoter
     CDS             504..1160
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2049..2067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2438..2625)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2649..3362)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(3382..4114)
                     /label=TR-SSRE promoter
                     /note="TR-SSRE promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4124..4140)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4160..4178)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4199..4215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4223..4239)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4247..4277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4292..4313)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4601..5189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5363..6220)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6221..6325)
                     /label=AmpR promoter
     misc_feature    6362..6865
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt
     2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
     2161 tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt
     2221 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
     2281 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
     2341 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa
     2401 acagatctat attaccctgt tatccctagc ggatctgccg gtagaggtgt ggtcaataag
     2461 agcgacctca tactatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
     2521 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
     2581 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtggc
     2641 gcgccttatt tgtacaattc atccatacca tgggtaatac cagcagcagt aacaaattct
     2701 aacaagacca tgtggtctct cttttcgttt ggatctttgg ataaggcaga ttgagtggat
     2761 aagtaatggt tgtctggtaa caagactgga ccatcaccaa ttggagtatt ttgttgataa
     2821 tggtcagcta attgaacaga accatcttca atgttgtgtc taattttgaa gttaactttg
     2881 ataccattct tttgtttgtc agccatgatg taaacattgt gagagttata gttgtattcc
     2941 aatttgtgac ctaaaatgtt accatcttct ttaaaatcaa taccttttaa ttcgattcta
     3001 ttaactaagg tatcaccttc aaacttgact tcagctctgg tcttgtagtt accgtcatct
     3061 ttgaaaaaaa tagttctttc ttgaacataa ccttctggca tggcagactt gaaaaagtca
     3121 tgttgtttca tatgatctgg gtatctcgca aaacattgaa caccataacc gaaagtagtg
     3181 actaaggttg gccatggaac tggcaattta ccagtagtac aaataaattt taaggtcaat
     3241 ttaccgtaag tagcatcacc ttcaccttca ccggagacag aaaatttgtg accattaaca
     3301 tcaccatcta attcaaccaa aattgggaca acaccagtga ataattcttc acctttagac
     3361 atttaattaa cccggggatc ccgtcgttgc ttttattacc gttgctctcc tagaatcaag
     3421 taagaaatta tcgaaaacct tatacagtaa gaagtttatg ttttattgag gacttaagta
     3481 cagtttttat agtttttata gttttcataa tttttattca gctacttcgt tcaatgtctt
     3541 taagattctt gatttgtaga cgtcaagaac cattttttgt tggataatgt aatacatgtc
     3601 ttcctttctt cttctcgagg cccagccaaa tctaccggtg tgggccggcc aaattcgagg
     3661 cccagccaaa tctaccggtg tgggccggcc aaattcgagt gaaagccaat gcgtcctgac
     3721 tattccctgg aaaaaccttt cagggatggg atgtctttct cccggcacat taatgtcgat
     3781 taaacacgag aatgcattaa aacaaacatg ttatgtttat ggcagagact agacgcattc
     3841 agaccaccat gtgttttcca gatttagtca tctcctagta atggtgttta cttagaagtc
     3901 gacttcggta gggtgctatc ctttaaaggc ccaccctgag cgtacagaaa gaattctagg
     3961 ggaagacggg gtagaaactt agtgttgatg tctcctgaag acttgttgnc tttggcaata
     4021 gcagcagcaa aaaaaaatgg gggttttaca caaatttcca aagtcttatc atcacgtgca
     4081 tttttcagta tacataatat taaagtaaaa aagaaagctt ggcactggcc gtcgttttac
     4141 agagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac ataggagccg
     4261 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt
     4321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5041 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt
     6361 catcacgtgc tataaaaata attataattt aaatttttta atataaatat ataaattaaa
     6421 aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa
     6481 gactctaggg ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta
     6541 ttaatgccga attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt
     6601 ttacatttta cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat
     6661 aaatatatat gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt
     6721 tcttcattcc gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca
     6781 taaaaataaa taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc
     6841 gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca ttattatcat gacattaacc
     6901 tataaaaata ggcgtatcac gaggcccttt cgtc
//
