LOCUS       40924_2757        6894 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector p413-SSRE-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Artificial cell-cell communication in yeast Saccharomyces cerevisiae
            using signaling elements from Arabidopsis thaliana
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Chen M-T., Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2005) Electrical Engineering, Princeton 
            University, Engineering Quadrangle, Olden Street, Princeton, NJ 
            08544, USA
REFERENCE   3  (bases 1 to 6894)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2005) Electrical Engineering, Princeton University, 
            Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        317..503
                     /label=HIS3 promoter
     CDS             504..1160
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(1423..1878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2023..2039
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2049..2067
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(2438..2625)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2649..3362)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(3382..4074)
                     /label=SSRE promoter
                     /note="SSRE promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4084..4100)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4120..4138)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4159..4175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4183..4199)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4207..4237)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4252..4273)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4561..5149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5323..6180)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6181..6285)
                     /label=AmpR promoter
     misc_feature    6322..6825
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt
     2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg
     2161 tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt
     2221 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
     2281 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
     2341 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa
     2401 acagatctat attaccctgt tatccctagc ggatctgccg gtagaggtgt ggtcaataag
     2461 agcgacctca tactatacct gagaaagcaa cctgacctac aggaaagagt tactcaagaa
     2521 taagaatttt cgttttaaaa cctaagagtc actttaaaat ttgtatacac ttattttttt
     2581 tataacttat ttaataataa aaatcataaa tcataagaaa ttcgcttatt tagaagtggc
     2641 gcgccttatt tgtacaattc atccatacca tgggtaatac cagcagcagt aacaaattct
     2701 aacaagacca tgtggtctct cttttcgttt ggatctttgg ataaggcaga ttgagtggat
     2761 aagtaatggt tgtctggtaa caagactgga ccatcaccaa ttggagtatt ttgttgataa
     2821 tggtcagcta attgaacaga accatcttca atgttgtgtc taattttgaa gttaactttg
     2881 ataccattct tttgtttgtc agccatgatg taaacattgt gagagttata gttgtattcc
     2941 aatttgtgac ctaaaatgtt accatcttct ttaaaatcaa taccttttaa ttcgattcta
     3001 ttaactaagg tatcaccttc aaacttgact tcagctctgg tcttgtagtt accgtcatct
     3061 ttgaaaaaaa tagttctttc ttgaacataa ccttctggca tggcagactt gaaaaagtca
     3121 tgttgtttca tatgatctgg gtatctcgca aaacattgaa caccataacc gaaagtagtg
     3181 actaaggttg gccatggaac tggcaattta ccagtagtac aaataaattt taaggtcaat
     3241 ttaccgtaag tagcatcacc ttcaccttca ccggagacag aaaatttgtg accattaaca
     3301 tcaccatcta attcaaccaa aattgggaca acaccagtga ataattcttc acctttagac
     3361 atttaattaa cccggggatc ccgtcgttgc ttttattacc gttgctctcc tagaatcaag
     3421 taagaaatta tcgaaaacct tatacagtaa gaagtttatg ttttattgag gacttaagta
     3481 cagtttttat agtttttata gttttcataa tttttattca gctacttcgt tcaatgtctt
     3541 taagattctt gatttgtaga cgtcaagaac cattttttgt tggataatgt aatacatgtc
     3601 ttcctttctt cttctcgagg cccagccaaa tctaccggtg tgggccggcc aaattcgagt
     3661 gaaagccaat gcgtcctgac tattccctgg aaaaaccttt cagggatggg atgtctttct
     3721 cccggcacat taatgtcgat taaacacgag aatgcattaa aacaaacatg ttatgtttat
     3781 ggcagagact agacgcattc agaccaccat gtgttttcca gatttagtca tctcctagta
     3841 atggtgttta cttagaagtc gacttcggta gggtgctatc ctttaaaggc ccaccctgag
     3901 cgtacagaaa gaattctagg ggaagacggg gtagaaactt agtgttgatg tctcctgaag
     3961 acttgttgnc tttggcaata gcagcagcaa aaaaaaatgg gggttttaca caaatttcca
     4021 aagtcttatc atcacgtgca tttttcagta tacataatat taaagtaaaa aagaaagctt
     4081 ggcactggcc gtcgttttac agagctccag cttttgttcc ctttagtgag ggttaattgc
     4141 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4201 tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4261 gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5641 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5701 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5761 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5821 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5881 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5941 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6001 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6061 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6121 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6181 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6241 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6301 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     6361 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     6421 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     6481 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     6541 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     6601 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     6661 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     6721 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     6781 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca
     6841 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
