LOCUS       40924_2647        3256 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p3E-zfRab18a-L24Q, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Nixon SJ, Hall TE, Parton RG.
  TITLE     Rab18 in zebrafish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Hall TE, Parton RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2014) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 3256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JUN-2014) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             747..1364
                     /codon_start=1
                     /product="Rab18a"
                     /label=Rab18a
                     /note="with L24Q mutation; derived from zebrafish"
                     /protein_id="AIO08312.1"
                     /translation="MNDDILTTLKILIIGESGVGKSSQLLRFTDDTFDAEIGATIGVDF
                     KVKTLAVDGNRAKLAIWDTAGQERFRTLTPSYYRGAQGVILVYDVSRRETFAKLDNWLS
                     ELETYCTRNDLVKMLVGNKIDKDDREIEREEGLKFARKHSMLFIESSAKTRDGVQCAFE
                     ELVEKILQTPGLWESMHKSRGVALSELSESSQGGCGAYCSLL"
     protein_bind    complement(1365..1460)
                     /label=attL3
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1498..1516)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1521..1537)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1650..2456
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2606..3194
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtga atgatgatat attgaccact ctgaaaatac
      781 tgatcattgg cgaaagtgga gtaggcaaat ccagtcagct tttgagattt actgatgata
      841 catttgatgc agaaattggt gcaacaatag gtgtggattt caaggtgaaa acgctggctg
      901 tagatggaaa ccgagcaaaa ctagcaatct gggatacagc aggtcaagaa cgttttcgga
      961 ctttaacacc cagttactac agaggagctc agggtgtcat tctggtgtat gatgtctcta
     1021 ggcgagagac atttgctaag ctggataact ggttgagtga gctggagacg tactgtacaa
     1081 gaaatgatct tgtcaagatg ctagtgggaa acaaaatcga taaggatgat cgtgagattg
     1141 agcgagagga gggcctgaag ttcgcaagaa aacactccat gcttttcata gagtccagtg
     1201 ctaaaacaag ggatggtgtc cagtgtgcct ttgaggagct ggtggagaaa atcctgcaga
     1261 ctccaggctt gtgggagagt atgcataagt caagaggagt tgccctgtct gaactctctg
     1321 aaagtagtca aggaggctgc ggggcgtact gctcacttct ctaacaactt tattatacaa
     1381 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1441 tcaaaataaa atcattattt ggagctccat ggtagcgtta acgcggccgc gatatcccct
     1501 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1561 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1621 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     1681 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1741 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1801 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1861 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1921 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1981 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     2041 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     2101 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     2161 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     2221 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     2281 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     2341 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     2401 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     2461 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     2521 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     2581 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2641 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2701 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2761 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2821 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2881 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2941 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     3001 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3061 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3121 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3181 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3241 cttttgctca catgtt
//
