LOCUS       62056_2045       11833 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11833)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        500..845
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1560..2276
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      2286..2927
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     misc_feature    3154..3178
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4478..5104
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5541..6605
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6674..6868
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7212..7352
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7538..8126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8216..9007)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9432..9456
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9534..9708)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9751..10773)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10841..11518)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11709..11730
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11745..11775
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11783..11799
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11807..11823
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcggtcc ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
       61 gagaggctta cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa
      121 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      181 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      241 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
      301 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
      361 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
      421 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
      481 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
      541 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
      601 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      661 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      721 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      781 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      841 gaacacgggg gacgagctcg gtacccggga tcctctagag ggcccggcgc gccactagta
      901 tggaaacata taatcataca tatagacata gaaatttcag tcataaagat ttaagtgatt
      961 taacatttac cgcttgtaca tttataagaa gcgatttcag aagagccaac ttaagagata
     1021 cgacatttgt gaattgtaaa tttatagaac aaggggatat agaaggatgc cattttgatg
     1081 ttgccgattt aagagatgca tcatttcaac aatgtcaatt agcaatggct aattttagta
     1141 atgcaaattg ttatggtata gaattcagag cttgtgattt aaagggagct aattttagca
     1201 gaacaaattt tgctcatcaa gtgagtaata gaatgtattt ttgcagcgct tttatatctg
     1261 gatgtaattt aagctatgct aatatggaaa gagtatgttt agaaaagtgt gaattgtttg
     1321 aaaatagatg gataggtaca aatttggcag gagctagttt aaaagaaagt gatttgagca
     1381 gaggtgtttt tagcgaagac gtgtggggac aattttcatt acaaggggct aatttatgcc
     1441 atgctgaatt agacggttta gatccaagaa aagtggatac ttcaggaata aaaatagctg
     1501 cttggcaaca agaattaata ttagaagctt taggaatcgt ggtgtatcca gatacgcgta
     1561 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1621 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1681 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1741 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1801 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     1861 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     1921 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1981 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2041 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2101 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2161 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2221 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgac
     2281 tgcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     2341 ttcattgcgc acacaccaga atcctactga gttcgagtat tatggcattg ggaaacatgt
     2401 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     2461 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     2521 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaaatat
     2581 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
     2641 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
     2701 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
     2761 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
     2821 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
     2881 ttttaatgca ttttatgact tgccaattga ttgacaacat gcatcaagct tggcactggc
     2941 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     3001 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     3061 ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt
     3121 cgtttcccgc cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct
     3181 aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc
     3241 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
     3301 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
     3361 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
     3421 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
     3481 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
     3541 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
     3601 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
     3661 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
     3721 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
     3781 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
     3841 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
     3901 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
     3961 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
     4021 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
     4081 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
     4141 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
     4201 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
     4261 tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
     4321 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
     4381 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
     4441 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
     4501 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
     4561 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
     4621 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
     4681 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
     4741 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
     4801 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
     4861 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
     4921 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
     4981 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
     5041 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
     5101 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
     5161 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
     5221 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
     5281 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
     5341 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
     5401 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
     5461 ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa
     5521 gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc
     5581 tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg
     5641 aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat
     5701 cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc
     5761 aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca
     5821 tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct
     5881 acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt
     5941 gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc
     6001 gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca
     6061 agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc
     6121 ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg
     6181 tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg
     6241 ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag
     6301 gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg
     6361 gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt
     6421 tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg
     6481 tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc
     6541 aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg
     6601 gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa
     6661 aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc
     6721 ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga
     6781 ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa
     6841 ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc
     6901 aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta
     6961 tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg
     7021 gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg
     7081 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     7141 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     7201 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     7261 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     7321 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     7381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca
     8221 attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt
     8281 caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga
     8341 aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta
     8401 ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt
     8461 cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag
     8521 tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca
     8581 attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt
     8641 gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt
     8701 catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat
     8761 catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca
     8821 tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta
     8881 aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac attccttccg
     8941 tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt
     9001 tagccattta ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa
     9061 ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa
     9121 aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat
     9181 tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac
     9241 cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc
     9301 atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg
     9361 actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     9421 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     9481 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
     9541 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     9601 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
     9661 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
     9721 gatcgacaga tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg
     9781 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
     9841 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
     9901 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
     9961 aagaccaatg cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct
    10021 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
    10081 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
    10141 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
    10201 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
    10261 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
    10321 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
    10381 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag
    10441 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
    10501 gatgcaatag gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag
    10561 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
    10621 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
    10681 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
    10741 gacagacgtc gcggtgagtt caggcttttt catatctcat tgccccccgg gatctgcgaa
    10801 agctcgagag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
    10861 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
    10921 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    10981 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    11041 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    11101 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    11161 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    11221 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
    11281 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    11341 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    11401 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    11461 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    11521 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    11581 ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg
    11641 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    11701 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    11761 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    11821 gaccatgatt acg
//
