LOCUS       62056_2030       16196 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..16196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(148..516)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(549..658)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(1342..1989)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    complement(2454..2478)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2634..2817
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2841..3629
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4859..4889
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        4975..5320
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             5340..6989
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    7049..7225
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             7283..9091
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     misc_feature    complement(10060..10084)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11683..12828)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13130..13921)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14113..14880
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(15581..16191)
                     /direction=LEFT
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
ORIGIN
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttcagaa gaccaaaggg ctattgagac
     4981 ttttcaacaa agggtaattt cgggaaacct cctcggattc cattgcccag ctatctgtca
     5041 cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa
     5101 aggaaaggct atcattcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc
     5161 cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg
     5221 atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc
     5281 ttcctctata taaggaagtt catttcattt ggagaggaca gcggtggaga tcgaattcca
     5341 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
     5401 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
     5461 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
     5521 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
     5581 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
     5641 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
     5701 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
     5761 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
     5821 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     5881 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     5941 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     6001 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     6061 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     6121 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     6181 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     6241 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     6301 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     6361 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     6421 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     6481 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     6541 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     6601 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     6661 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     6721 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     6781 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     6841 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     6901 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     6961 aggccaagaa gggcggaaag atcgccgtgt aattctagag aattcgctga aatcaccagt
     7021 ctctctctac aaatctatct ctctctattt tctccataaa taatgtgtga gtagtttccc
     7081 gataagggaa attagggttc ttatagggtt tcgctcatgt gttgagcata taagaaaccc
     7141 ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc
     7201 aaaatccagt actaaaatcc agatccacta gcctcctcga ggtcgagtcg actctagagg
     7261 atccccgggt ggtcagtccc ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa
     7321 aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg
     7381 ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca gttttaacga
     7441 tcagttcgcc gatgcagata ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt
     7501 ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg cggtcactca
     7561 ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg gctatacgcc
     7621 atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg
     7681 tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta ccgacgaaaa
     7741 cggcaagaaa aagcagtctt acttccatga tttctttaac tatgccggaa tccatcgcag
     7801 cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt
     7861 cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag
     7921 cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca ctagcgggac
     7981 tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct atgaactgtg
     8041 cgtcacagcc aaaagccaga cagagtgtga tatctacccg cttcgcgtcg gcatccggtc
     8101 agtggcagtg aagggcgaac agttcctgat taaccacaaa ccgttctact ttactggctt
     8161 tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc gataacgtgc tgatggtgca
     8221 cgaccacgca ttaatggact ggattggggc caactcctac cgtacctcgc attaccctta
     8281 cgctgaagag atgctcgact gggcagatga acatggcatc gtggtgattg atgaaactgc
     8341 tgctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga
     8401 actgtacagc gaagaggcag tcaacgggga aactcagcaa gcgcacttac aggcgattaa
     8461 agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta ttgccaacga
     8521 accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg aagcaacgcg
     8581 taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg acgctcacac
     8641 cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg gatggtatgt
     8701 ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc tggcctggca
     8761 ggagaaactg catcagccga ttatcatcac cgaatacggc gtggatacgt tagccgggct
     8821 gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc tggatatgta
     8881 tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga atttcgccga
     8941 ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga tcttcactcg
     9001 cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg gcatgaactt
     9061 cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac aactctcctg gcgcaccatc
     9121 gtcggctaca gcctcgggaa ttgctaccga gctcgaattt ccccgatcgt tcaaacattt
     9181 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     9241 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     9301 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     9361 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     9421 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9481 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9541 gatcgccctt cccaacagtt gcgcagcctg aatggcgccc gctcctttcg ctttcttccc
     9601 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9661 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg
     9721 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9781 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta
     9841 ttcttttgat ttataaggga ttttgccgat ttcggaacca ccatcaaaca ggattttcgc
     9901 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     9961 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt acattaaaaa
    10021 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    10081 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    10141 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
    10201 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
    10261 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
    10321 caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat ttctcgctta
    10381 accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc gctggataaa
    10441 gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg atatcaactc
    10501 ccctatccat tgctcaccga atggtacagg tcggggaccc gaagttccga ctgtcggcct
    10561 gatgcatccc cggctgatcg accccagatc tggggctgag aaagcccagt aaggaaacaa
    10621 ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga
    10681 tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat
    10741 caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa
    10801 aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg
    10861 aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca
    10921 acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta
    10981 tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta
    11041 ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata
    11101 gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct
    11161 gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga
    11221 cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt
    11281 ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg
    11341 cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct
    11401 cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct
    11461 cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg
    11521 tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata
    11581 ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct
    11641 ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag
    11701 gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc
    11761 gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa
    11821 ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg
    11881 ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc
    11941 aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg
    12001 gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga
    12061 cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata
    12121 ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg
    12181 ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc
    12241 gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat
    12301 gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga
    12361 gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa
    12421 ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc
    12481 ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt
    12541 tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg
    12601 cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc
    12661 gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg
    12721 gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc
    12781 gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg
    12841 cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg
    12901 tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg
    12961 gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt
    13021 gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc
    13081 ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa aacaattcat
    13141 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
    13201 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
    13261 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
    13321 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
    13381 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
    13441 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
    13501 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
    13561 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
    13621 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
    13681 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
    13741 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
    13801 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
    13861 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
    13921 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
    13981 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
    14041 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
    14101 accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
    14161 tgctccagtg gcttctgtgt ctatcagctg tccctcctgt tcagctactg acggggtggt
    14221 gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact gccgtaaaac
    14281 atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga aaatcattga
    14341 tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg ttggcctcaa
    14401 cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg catacagccg
    14461 ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt cggggctaaa
    14521 tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt tgttgcgcac
    14581 gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct gtcacccttt
    14641 gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct gaagggaaag
    14701 ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct gaatctgagg
    14761 cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat
    14821 gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc
    14881 caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag cattagtcca
    14941 tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct gcctatgcct
    15001 tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata ttatcttatc
    15061 agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg
    15121 gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat tctcgttttc
    15181 atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat cgcacccccg
    15241 aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa aattgccggc
    15301 cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc gccacccagg
    15361 ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg
    15421 tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg
    15481 gcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc ggccgagggg
    15541 cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc gagaaggggg
    15601 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
    15661 gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc cgaaaaacgg
    15721 gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa atgtcaatag
    15781 gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg cccctcatct
    15841 gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca ggcttgtcca
    15901 catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg aggctggcca
    15961 gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg cgccgggtga
    16021 gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca agttttccgc
    16081 gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg
    16141 cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca gcccgg
//
