LOCUS       40924_2612        3394 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34S-Tp2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Improved antibiotic-resistance cassettes through restriction site 
            elimination using Pfu DNA polymerase PCR
  JOURNAL   BioTechniques 25 (5), 772-774 (1998)
  PUBMED    9821576
REFERENCE   3  (bases 1 to 3394)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-1998) Biotechnology Center for Agriculture and the
            Environment, Cook College, Rutgers University, 59 Dudley Rd., New 
            Brunswick, NJ 08901, USA
REFERENCE   4  (bases 1 to 3394)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3394)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "5"; pages: 
            "772-774"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-1998) Biotechnology Center for Agriculture and the 
            Environment, Cook College, Rutgers University, 59 Dudley Rd., New 
            Brunswick, NJ 08901, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site cassette
                     /note="multiple cloning site cassette"
     promoter        64..92
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_difference 374
                     /replace="c"
                     /label=site-directed modification
                     /note="site-directed modification"
     CDS             419..652
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     misc_feature    658..708
                     /label=multiple cloning site cassette
                     /note="multiple cloning site cassette"
     promoter        complement(714..732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(751..767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(775..791)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(799..829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(844..865)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1153..1741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1915..2772)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2773..2877)
                     /label=AmpR promoter
     primer_bind     3351..3367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3370..3388
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgcacgaacc
       61 cagttgacat aagcctgttc ggttcgtaaa ctgtaatgca agtagcgtat gcgctcacgc
      121 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
      181 gcttgttatg actgtttttt tgtacagtct agcctcgggc atccaagcta gctaagcgcg
      241 ttacgccgtg ggtcgatgtt tgatgttatg gaacagcaac gatgttacgc agcagggtag
      301 tcgccctaaa acaaagttag gcagccgttg tgctggtgct ttctagtagt tgttgtgggg
      361 taggcagtca gaggtcgatt tgcttgtcgc cataatagat tcacaagaag gattcgacat
      421 gggtcaaagt agcgatgaag ccaacgctcc cgttgcaggg cagtttgcgc ttcccctgag
      481 tgccaccttt ggcttagggg atcgcgtacg caagaaatct ggtgccgctt ggcagggtca
      541 agtcgtcggt tggtattgca caaaactcac tcctgaaggc tatgcggtcg agtccgaatc
      601 ccacccaggc tcagtgcaaa tttatcctgt ggctgcactt gaacgtgtgg cctaagcgag
      661 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgt ctccctatag
      721 tgagtcgtat tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      781 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      841 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tcgagtcggg
      901 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      961 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1141 gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1321 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1561 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     1621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgg
     2221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2881 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2941 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     3001 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     3061 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     3121 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     3181 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
     3241 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     3301 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     3361 ggccagtgaa tttaggtgac actatagaat actc
//
