LOCUS       40924_2602        4154 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34S-Tc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-1998) Biotechnology Center for Agriculture and the
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA
REFERENCE   3  (bases 1 to 4154)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4154)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-APR-1998) Biotechnology Center for Agriculture and the 
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     promoter        56..85
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             133..1320
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     misc_feature    1418..1468
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     promoter        complement(1474..1492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1511..1527)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1535..1551)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1559..1589)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1604..1625)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1913..2501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2675..3532)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3533..3637)
                     /label=AmpR promoter
     primer_bind     4111..4127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4130..4148
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct catgtttgac
       61 agcttatcat ccgattagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt
      121 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
      181 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
      241 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
      301 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
      361 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
      421 tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
      481 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      541 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
      601 atctccttac atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
      661 ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gaccgatgcc cttgagagcc
      721 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
      781 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
      841 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
      901 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
      961 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     1021 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     1081 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     1141 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg
     1201 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     1261 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     1321 tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc
     1381 aattcttgcg gagaactgtg aatgcgcaaa ccaacccgag ctcggtaccc ggggatcctc
     1441 tagagtcgac ctgcaggcat gcaagcttgt ctccctatag tgagtcgtat tagagcttgg
     1501 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1621 cattaattgc gttgcgctca ctgcccgctt tcgagtcggg aaacctgtcg tgccagctgc
     1681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttcgata
     1921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2341 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2641 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2701 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2761 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2821 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2881 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2941 taagtagttc gccagttaat agtttgcgca acgttgttgg cattgctaca ggcatcgtgg
     3001 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3061 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3121 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3181 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3241 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3301 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3361 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3421 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3481 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3541 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3601 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3661 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3721 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3841 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3961 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     4021 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     4081 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa tttaggtgac
     4141 actatagaat actc
//
