LOCUS       40924_2597        4503 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34S-Sm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-1998) Biotechnology Center for Agriculture and the
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA
REFERENCE   3  (bases 1 to 4503)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-APR-1998) Biotechnology Center for Agriculture and the 
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     CDS             492..1256
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEGG"
     misc_feature    1767..1817
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     promoter        complement(1823..1841)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1860..1876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1884..1900)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1908..1938)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1953..1974)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2262..2850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3024..3881)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3882..3986)
                     /label=AmpR promoter
     primer_bind     4460..4476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4479..4497
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgatgcttgt
       61 aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga cctctagggt ccccaattaa
      121 ttagtaatat aatctattaa aggtcattca aaaggtcatc caccggatca attcccctgc
      181 tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt ggagcccttg
      241 ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa
      301 ccctcactga tccggctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
      361 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag
      421 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
      481 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
      541 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
      601 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
      661 tgcgtgctgc gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
      721 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
      781 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
      841 ggccacaggc agagcagatc atctctgatc cattgcccct gccacctcac tcgcctgcaa
      901 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
      961 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     1021 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     1081 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     1141 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     1201 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag ggcgggatgc
     1261 gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga
     1321 cgttggaggg gcaaggtcgc gctgattgct ggggcaacac gtgaaaggcg agatcaccaa
     1381 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
     1441 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     1501 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     1561 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat
     1621 taaaatctag cgagggcttt actaagctga tccggtggat gaccttttga atgaccttta
     1681 atagattata ttactaatta attggggacc ctagaggtcc ccttttttat tttaaaaatt
     1741 ttttcacaaa acggtttaca agcatcgagc tcggtacccg gggatcctct agagtcgacc
     1801 tgcaggcatg caagcttgtc tccctatagt gagtcgtatt agagcttggc gtaatcatgg
     1861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1981 ttgcgctcac tgcccgcttt cgagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttcgatag gctccgcccc
     2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3301 ccagttaata gtttgcgcaa cgttgttggc attgctacag gcatcgtggt gtcacgctcg
     3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     4021 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     4081 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     4141 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4201 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     4261 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     4321 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     4381 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     4441 ttttcccagt cacgacgttg taaaacgacg gccagtgaat ttaggtgaca ctatagaata
     4501 ctc
//
