LOCUS       40924_2587        4026 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34S-Km, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-1998) Biotechnology Center for Agriculture and the
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA
REFERENCE   3  (bases 1 to 4026)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-APR-1998) Biotechnology Center for Agriculture and the 
            Environment, Cook College, Rutgers University, Foran Hall, 59 Dudley
            Rd., New Brunswick, NJ 08901-8520, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     CDS             175..987
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    1290..1340
                     /label=cassette multiple cloning site
                     /note="cassette multiple cloning site"
     promoter        complement(1346..1364)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1383..1399)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1407..1423)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1431..1461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1476..1497)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1785..2373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2547..3404)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3405..3509)
                     /label=AmpR promoter
     primer_bind     3983..3999
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4002..4020
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cggggggggg
       61 ggggaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
      121 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
      181 catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat
      241 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
      301 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      361 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      421 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      481 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      541 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
      601 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
      661 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
      721 cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
      781 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
      841 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
      901 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
      961 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     1021 cagagcatta cgctgacttg acgggacggc ggctttgttg aataaatcga acttttgctg
     1081 agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa
     1141 agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca
     1201 ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac
     1261 gaggcagacc tcagcgcccc cccccccccg agctcggtac ccggggatcc tctagagtcg
     1321 acctgcaggc atgcaagctt gtctccctat agtgagtcgt attagagctt ggcgtaatca
     1381 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     1441 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1501 gcgttgcgct cactgcccgc tttcgagtcg ggaaacctgt cgtgccagct gcattaatga
     1561 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1621 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1681 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1741 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcga taggctccgc
     1801 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1861 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1921 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1981 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2041 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2101 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2161 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2221 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2401 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2461 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2521 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     2581 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     2641 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     2701 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     2761 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     2821 tcgccagtta atagtttgcg caacgttgtt ggcattgcta caggcatcgt ggtgtcacgc
     2881 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     2941 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3001 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3061 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3121 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3181 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     3241 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     3301 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     3361 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     3421 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     3481 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     3541 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     3601 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3661 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3721 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     3781 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     3841 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     3901 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     3961 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatttaggtg acactataga
     4021 atactc
//
