LOCUS       40924_2577        3604 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p34S-Cm2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Improved antibiotic-resistance cassettes through restriction site 
            elimination using Pfu DNA polymerase PCR
  JOURNAL   BioTechniques 25 (5), 772-774 (1998)
  PUBMED    9821576
REFERENCE   3  (bases 1 to 3604)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-1998) Biotechnology Center for Agriculture and the
            Environment, Cook College, Rutgers University, 59 Dudley Rd., New 
            Brunswick, NJ 08901, USA
REFERENCE   4  (bases 1 to 3604)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3604)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "25"; issue: "5"; pages: 
            "772-774"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-1998) Biotechnology Center for Agriculture and the 
            Environment, Cook College, Rutgers University, 59 Dudley Rd., New 
            Brunswick, NJ 08901, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=multiple cloning site cassette
                     /note="multiple cloning site cassette"
     CDS             120..776
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_feature    868..918
                     /label=multiple cloning site cassette
                     /note="multiple cloning site cassette"
     promoter        complement(924..942)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(961..977)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(985..1001)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1009..1039)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1054..1075)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1363..1951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2125..2982)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2983..3087)
                     /label=AmpR promoter
     primer_bind     3561..3577
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3580..3598
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cttgaaataa
       61 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
      121 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      181 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      241 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      301 acattcttgc ccgcctgatg aatgctcatc cggagttccg tatggcaatg aaagacggtg
      361 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      421 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      481 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      541 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      601 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      661 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
      721 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
      781 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
      841 ataagcggat gaatggcaga aattcgcgag ctcggtaccc ggggatcctc tagagtcgac
      901 ctgcaggcat gcaagcttgt ctccctatag tgagtcgtat tagagcttgg cgtaatcatg
      961 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1021 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1081 gttgcgctca ctgcccgctt tcgagtcggg aaacctgtcg tgccagctgc attaatgaat
     1141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttcgata ggctccgccc
     1381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1801 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2041 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2101 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2161 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2221 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2281 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2341 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2401 gccagttaat agtttgcgca acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc
     2461 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2521 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2581 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2641 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2701 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2761 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2821 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2881 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2941 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3001 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3061 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3121 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3181 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3241 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3301 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3361 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     3421 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3481 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3541 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa tttaggtgac actatagaat
     3601 actc
//
