LOCUS       V007315                10729 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V007315
VERSION     V007315
KEYWORDS    p31sheLL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL,
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
   PUBMED   17603495
REFERENCE   2  (bases 1 to 10729)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10729)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med.";
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             20..1531
                     /gene="L1"
                     /label="Major capsid protein L1"
                     /note="Major capsid protein L1 from Human papillomavirus
                     31. Accession#: P17388"
     CDS             2218..3615
                     /gene="L2"
                     /label="Minor capsid protein L2"
                     /note="Minor capsid protein L2 from Human papillomavirus
                     31. Accession#: P17389"
     misc_feature    3666..4254
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4297..4521
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4567..4995
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5004..5024)
                     /label="pBABE 3'"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6953..7318
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7451..7584
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7621..7637)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(7621..7637)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7634..7656)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    7645..7661
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7669..7699)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7714..7735)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7852..7869)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8023..8608)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8782..9639)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9640..9744)
                     /label="AmpR promoter"
     primer_bind     complement(9819..9838)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        10010..10389
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10390..10593
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    10595..10613
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10616..10634
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10644..10668
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccggagggc cgcgccacca tgagcctgtg gaggcccagc gaggccaccg tgtacctgcc
       61 ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtgaccagga ccaacatcta
      121 ctaccacgcc ggcagcgcca ggctgctgac cgtgggccac ccctactaca gcatccccaa
      181 gagcgacaac cccaagaaga tcgtggtgcc caaggtgagc ggcctgcagt acagggtgtt
      241 cagggtgagg ctgcccgacc ccaacaagtt cggcttcccc gacaccagct tctacaaccc
      301 cgagacccag aggctggtgt gggcctgcgt gggcctggag gtgggcaggg gccagcccct
      361 gggcgtgggc atcagcggcc accccctgct gaacaagttc gacgacaccg agaacagcaa
      421 caggtacgcc ggcggccccg gcaccgacaa cagggagtgc atcagcatgg actacaagca
      481 gacccagctg tgcctgctgg gctgcaagcc ccccatcggc gagcactggg gcaagggcag
      541 cccctgcagc aacaacgcca tcacccccgg cgactgcccc cccctggagc tgaagaacag
      601 cgtgatccag gacggcgaca tggtggacac cggcttcggc gccatggact tcaccgccct
      661 gcaggacacc aagagcaacg tgcccctgga catctgcaac agcatctgca agtaccccga
      721 ctacctgaag atggtggccg agccctacgg cgacaccctg ttcttctacc tgaggaggga
      781 gcagatgttc gtgaggcact tcttcaacag gagcggcacc gtgggcgaga gcgtgcccac
      841 cgacctgtac atcaagggca gcggcagcac cgccaccctg gccaacagca cctacttccc
      901 cacccccagc ggcagcatgg tgaccagcga cgcccagatc ttcaacaagc cctactggat
      961 gcagagggcc cagggccaca acaacggcat ctgctggggc aaccagctgt tcgtgaccgt
     1021 ggtggacacc accaggagca ccaacatgag cgtgtgcgcc gccatcgcca acagcgacac
     1081 caccttcaag agcagcaact tcaaggagta cctgaggcac ggcgaggagt tcgacctgca
     1141 gttcatcttc cagctgtgca agatcaccct gagcgccgac atcatgacct acatccacag
     1201 catgaacccc gccatcctgg aggactggaa cttcggcctg accacccccc ccagcggcag
     1261 cctggaggac acctacaggt tcgtgaccag ccaggccatc acctgccaga agaccgcccc
     1321 ccagaagccc aaggaggacc ccttcaagga ctacgtgttc tgggaggtga acctgaagga
     1381 gaagttcagc gccgacctgg accagttccc cctgggcagg aagttcctgc tgcaggccgg
     1441 ctacagggcc aggcccaagt tcaaggccgg caagaggagc gcccccagcg ccagcaccac
     1501 cacccccgcc aagaggaaga agaccaagaa gtaaaagctt tttgaattct ttggatccac
     1561 tagggctcga ggaagcttaa aacagctctg gggttgtacc caccccagag gcccacgtgg
     1621 cggctagtac tccggtattg cggtaccctt gtacgcctgt tttatactcc cttcccgtaa
     1681 cttagacgca caaaaccaag ttcaatagaa gggggtacaa accagtacca ccacgaacaa
     1741 gcacttctgt ttccccggtg atgtcgtata gactgcttgc gtggttgaaa gcgacggatc
     1801 cgttatccgc ttatgtactt cgagaagccc agtaccacct cggaatcttc gatgcgttgc
     1861 gctcagcact caaccccaga gtgtagctta ggctgatgag tctggacatc cctcaccggt
     1921 gacggtggtc caggctgcgt tggcggccta cctatggcta acgccatggg acgctagttg
     1981 tgaacaaggt gtgaagagcc tattgagcta cataagaatc ctccggcccc tgaatgcggc
     2041 taatcccaac ctcggagcag gtggtcacaa accagtgatt ggcctgtcgt aacgcgcaag
     2101 tccgtggcgg aaccgactac tttgggtgtc cgtgtttcct tttattttat tgtggctgct
     2161 tatggtgaca atcacagatt gttatcataa agcgaattgg attgcggccg cgccaccatg
     2221 aggagcaaga ggagcaccaa gaggaccaag agggccagcg ccacccagct gtaccagacc
     2281 tgcaaggccg ccggcacctg ccccagcgac gtgatcccca agatcgagca caccaccatc
     2341 gccgaccaga tcctgaggta cggcagcatg ggcgtgttct tcggcggcct gggcatcggc
     2401 agcggcagcg gcaccggcgg caggaccggc tacgtgcccc tgagcaccag gcccagcacc
     2461 gtgagcgagg ccagcatccc catcaggccc cccgtgagca tcgaccccgt gggccccctg
     2521 gaccccagca tcgtgagcct ggtggaggag agcggcatcg tggacgtggg cgcccccgcc
     2581 cccatccccc acccccccac caccagcggc ttcgacatcg ccaccaccgc cgacaccacc
     2641 cccgccatcc tggacgtgac cagcgtgagc acccacgaga accccacctt caccgacccc
     2701 agcgtgctgc agccccccac ccccgccgag accagcggcc acctgctgct gagcagcagc
     2761 agcatcagca cccacaacta cgaggagatc cccatggaca ccttcatcgt gagcaccaac
     2821 aacgagaaca tcaccagcag cacccccatc cccggcgtga ggaggcccgc caggctgggc
     2881 ctgtacagca aggccaccca gcaggtgaag gtgatcgacc ccaccttcct gagcgccccc
     2941 aagcagctga tcacctacga gaaccccgcc tacgagaccg tgaacgccga ggagagcctg
     3001 tacttcagca acaccagcca caacatcgcc cccgaccccg acttcctgga catcatcgcc
     3061 ctgcacaggc ccgccctgac cagcaggagg aacaccgtga ggtacagcag gctgggcaac
     3121 aagcagaccc tgaggaccag gagcggcgcc accatcggcg ccagggtgca ctactactac
     3181 gacatcagca gcatcaaccc cgccggcgag agcatcgaga tgcagcccct gggcgccagc
     3241 gccaccacca ccagcaccct gaacgacggc ctgtacgaca tctacgccga caccgacttc
     3301 accgtggaca cccccgccac ccacaacgtg agccccagca ccgccgtgca gagcaccagc
     3361 gccgtgagcg cctacgtgcc caccaacacc accgtgcccc tgagcaccgg cttcgacatc
     3421 cccatcttca gcggccccga cgtgcccatc gagcacgccc ccacccaggt gttccccttc
     3481 cccctggccc ccaccacccc ccaggtgagc atcttcgtgg acggcggcga cttctacctg
     3541 caccccagct actacatgct gaagaggagg aggaagaggg tgagctactt cttcaccgac
     3601 gtgagcgtgg ccgcctgaaa gcttttgaat tctttggatc cactagcgat atcaagctta
     3661 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3721 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3781 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3841 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3901 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3961 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4021 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     4081 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4141 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4201 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     4261 accgtcggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     4321 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     4381 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     4441 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     4501 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     4561 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4981 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     5041 ctccccagca ggcagaagta tgcaaagcat gcagaattct atcaaatatt taaagaaaaa
     5101 aaaattgtat caactttcta caatctcttt cagaagacag aagcagaggg aatacttcct
     5161 aaatcattca actaggccag cattacctta ataccggaac tagaaaatga cattacaaga
     5221 aaagaaaaca acagaccaat atctctcatg aacaaagata caaacatttt caacaaaata
     5281 ttagcaaaaa gaatccaaga atgtatcaaa aaatatacac cacaaccaag tagaatttat
     5341 tccagatatg taagggtggt tcaacgtttg aaaatcaatt aacgtaattt gtcccatcaa
     5401 caggttaaag aagaaaatca catggtcata ttgatagaca cagaaaaagc atttgacaaa
     5461 atttaacacc cattcatgat gcaatctctc agtaaactag gaatagagga aaacttcctc
     5521 agcttgaatg taccttcctc tcaattttgc tatgaacctg aaactcctct taaaaaataa
     5581 agtttttcat ttaaaaagaa aacaaaaaac atggaggagc gttgatgtat ctcattttag
     5641 accaatcagc tatggatagt taggcgacag cacagatagc tgctgtactt ctgtttctgg
     5701 caatgttcca gactacattt aaaaaatttt taattataga cttgtactta atgttcaaga
     5761 aaaatatgaa aatggctttg ccgtgttaat gctactcttt tttaaaaaaa actaaagttc
     5821 aaactttatt tatatttcat tagtttttta gctactgttc tttttctgtt ctgggatctc
     5881 attcagaatg ccacattaca tataattctc atgtctcctt gggttcctct tagttttgac
     5941 agttcctcag acttttctta tttttgatga ccttgacagt tttgaggagt actggttaga
     6001 tatagggtaa tggtttttaa agtatatttg tcatgattta tactggggta agggtttggg
     6061 gaggaagccc atggggtaaa gtactgttct catcacatca tatcaaggtt atataccatc
     6121 aatattgcca cagatgttac ttagcctttt aatatttctc taatttagtg tatatgcaat
     6181 gatagttctc tgatttctga gattgagttt ctcatgtgta atgattattt agagtttctc
     6241 tttcatctgt tcaaattttt gtctagtttt attttttact gatttgtaag acttcttttt
     6301 ataatctgca tattacaatt ctctttactg gggtgttgca aatattttct gtcattctat
     6361 ggcctgactt ttcttaatgg ttttttaatt ttaaaaataa gtcttaatat tcatgcaatc
     6421 taattaacaa tcttttcttt gtggttagga ctttgagtca taagaaattt ttctctacac
     6481 tgaagtcatg atggcatgct tctatattat tttctaaaag atttaaagtt ttgccttctc
     6541 catttagact tataattcac tggaattttt ttgtgtgtat ggtatgacat atgggttccc
     6601 ttttattttt tacatataaa tatatttccc tgtttttcta aaaaagaaaa agatcatcat
     6661 tttcccattg taaaatgcca tatttttttc ataggtcact tacatatatc aatgggtctg
     6721 tttctgagct ctactctatt ttatcagcct cactgtctat ccccacacat ctcatgcttt
     6781 gctctaaatc ttgatattta gtggaacatt ctttcccatt ttgttctaca agaatatttt
     6841 tgttattgtc ttttgggctt ctatatacat tttagaatga ggttggcaag ttaacaaaca
     6901 gcttttttgg ggtgaacata ttgactacaa atttatgtgg aaagaaagta ccaagttgac
     6961 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     7021 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     7081 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     7141 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     7201 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     7261 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     7321 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7381 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7441 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7561 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7621 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7681 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7741 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7801 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7861 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7921 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7981 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8041 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8101 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8161 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8221 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8281 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8341 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8401 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8461 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8521 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     8581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     9781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     9841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     9901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     9961 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
    10021 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
    10081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
    10141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
    10201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
    10261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
    10321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
    10381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
    10441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gaaccaaaat
    10501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
    10561 cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct
    10621 atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc
    10681 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcc
//
