LOCUS       62056_1995        5332 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             931..996
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    1673..2295
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        2324..2653
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2692..2710)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2724..2740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2748..2764)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2772..2802)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2817..2838)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3126..3714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3888..4745)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4746..4850)
                     /label=AmpR promoter
     rep_origin      4877..5332
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa
      961 gatcatgaca tcgattacaa ggatgacgat gacaagcttg cggccgcgaa ttcgatgagc
     1021 tcgaaagttc cttatgagat acaaaaagga caagcactta tcacctttga aaaagaagaa
     1081 gttgctcaaa atgtggtaag catgagtaaa catcatgtac agataaaaga tgtaaatctg
     1141 gaggttacgg ccaagccagt tccattaaat tcaggagtca gattccaggt ttatgtagaa
     1201 gtttctaaaa tgaaaatcaa tgttactgaa attcctgaca cattgcgtga agatcaaatg
     1261 agagacaaac tagagctgag cttttcaaag tcccgaaatg gaggcggaga ggtggaccgc
     1321 gtggactatg acagacagtc cgggagtgca gtcatcacgt ttgtggagat tggagtggct
     1381 gacaagattt tgaaaaagaa agaataccct ctttatataa atcaaacctg ccatagagtt
     1441 actgtttctc catacacaga aatacacttg aaaaagtatc agatattttc aggaacatct
     1501 aagaggacag tgcttctgac aggaatggaa ggcattcaaa tggatgaaga aattgtggag
     1561 gatttaatta acattcactt tcaacgggca aagaatggag gtggagaagt agatgtggtc
     1621 aagtgttctc taggtcaacc tcacatagca tactttgaag aatgaggatc ccgggtggca
     1681 tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc cagtgcccac
     1741 cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt ccttctataa
     1801 tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga caacctgtag
     1861 ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt ggctcactgc
     1921 aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt tgttgggatt
     1981 ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac ggggtttcac
     2041 catattggcc aggctggtct ccaactccta atctcaggtg atctacccac cttggcctcc
     2101 caaattgctg ggattacagg cgtgaaccac tgctcccttc cctgtccttc tgattttaaa
     2161 ataactatac cagcaggagg acgtccagac acagcatagg ctacctggcc atgcccaacc
     2221 ggtgggacat ttgagttgct tgcttggcac tgtcctctca tgcgttgggt ccactcagta
     2281 gatgcctgtt gaattgggta cgcggccagc ttggctgtgg aatgtgtgtc agttagggtg
     2341 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     2401 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     2461 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     2521 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     2581 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     2641 aggcttttgc aaaaagctcc tcgaggaact gaaaaaccag aaagttaatt ccctatagtg
     2701 agtcgtatta aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2761 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2821 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2881 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2941 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3001 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3061 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3121 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3181 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3241 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3301 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3361 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3421 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3481 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3541 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3601 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3661 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3721 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3781 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3841 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3901 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3961 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4021 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4081 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4141 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4201 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4261 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4321 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4381 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4441 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4501 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4561 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4621 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4681 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4741 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4801 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4861 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4921 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4981 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5041 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5101 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5161 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5221 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5281 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat tt
//
