LOCUS       p2luc.        5393 bp DNA     circular SYN 18-FEB-2022
DEFINITION  Reporter vector p2luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    p2luc.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Grentzmann G, Ingram JA, Kelly PJ, Gesteland RF, Atkins JF.
  TITLE     A dual-luciferase reporter system for studying recoding signals
  JOURNAL   RNA 4 (4), 479-486 (1998)
  PUBMED    9630253
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Grentzmann G, Gesteland RF, Atkins JF.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1998) HHMI, University of Utah, 6100 Eccles Bldg.,
            Salt Lake City, UT 84112, USA
REFERENCE   3  (bases 1 to 5393)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5393)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "1998"; volume: "4"; issue: "4"; pages: "479-486"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-JAN-1998) HHMI, University of Utah, 6100 Eccles Bldg., Salt Lake
            City, UT 84112, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..1626
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     CDS             1656..3305
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(3348..3469)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3602..3706
                     /label=AmpR promoter
     CDS             3707..4564
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4738..5326
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa
      721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt
      781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt
      841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca
      901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat
      961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat
     1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat
     1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg
     1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa
     1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa
     1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa
     1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt
     1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat
     1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt
     1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa
     1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat
     1621 gaacaaatgt cgacggatcc ttcaacttcc ctgagctcga agacgccaaa aacataaaga
     1681 aaggcccggc gccattctat cctctagagg atggaaccgc tggagagcaa ctgcataagg
     1741 ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg
     1801 tgaacatcac gtacgcggaa tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac
     1861 gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct
     1921 ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt
     1981 ataatgaacg tgaattgctc aacagtatga acatttcgca gcctaccgta gtgtttgttt
     2041 ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa attaccaata atccagaaaa
     2101 ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca
     2161 catctcatct acctcccggt tttaatgaat acgattttgt accagagtcc tttgatcgtg
     2221 acaaaacaat tgcactgata atgaattcct ctggatctac tgggttacct aagggtgtgg
     2281 cccttccgca tagaactgcc tgcgtcagat tctcgcatgc cagagatcct atttttggca
     2341 atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa
     2401 tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg
     2461 aagaagagct gtttttacga tcccttcagg attacaaaat tcaaagtgcg ttgctagtac
     2521 caaccctatt ttcattcttc gccaaaagca ctctgattga caaatacgat ttatctaatt
     2581 tacacgaaat tgcttctggg ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa
     2641 aacgcttcca tcttccaggg atacgacaag gatatgggct cactgagact acatcagcta
     2701 ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt
     2761 ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat cagagaggcg
     2821 aattatgtgt cagaggacct atgattatgt ccggttatgt aaacaatccg gaagcgacca
     2881 acgccttgat tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag
     2941 acgaacactt cttcatagtt gaccgcttga agtctttaat taaatacaaa ggatatcagg
     3001 tggcccccgc tgaattggaa tcgatattgt tacaacaccc caacatcttc gacgcgggcg
     3061 tggcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc
     3121 acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg
     3181 cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa
     3241 aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagtcca
     3301 aattgtaaca cgtgtaattc tagagcggcc gcttcgagca gacatgataa gatacattga
     3361 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     3421 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     3481 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     3541 aaacctctac aaatgtggta aaatcgataa ggatccaggt ggcacttttc ggggaaatgt
     3601 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     3661 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     3721 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     3781 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     3841 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     3901 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     3961 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4021 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4081 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4141 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4201 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4261 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4321 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4381 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4441 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4501 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4561 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     4621 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4681 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4741 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4801 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4861 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4921 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4981 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5041 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5101 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5161 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5221 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5281 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5341 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc gac
//
