LOCUS       Exported                6731 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Reporter vector p2luc, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    p2luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Grentzmann G, Ingram JA, Kelly PJ, Gesteland RF, Atkins JF.
  TITLE     A dual-luciferase reporter system for studying recoding signals
  JOURNAL   RNA 4 (4), 479-486 (1998)
  PUBMED    9630253
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Grentzmann G, Gesteland RF, Atkins JF.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1998) HHMI, University of Utah, 6100 Eccles Bldg.,
            Salt Lake City, UT 84112, USA
REFERENCE   3  (bases 1 to 6731)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6731)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "1998"; volume: "4"; issue: "4"; pages: "479-486"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-JAN-1998) HHMI, University of Utah, 6100 Eccles Bldg., Salt Lake
            City, UT 84112, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          151..1488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..1757
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        151..1488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          1827..1959
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        2004..2022
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2032..2964
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     CDS             2994..4643
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(4686..4807)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4940..5044
                     /label=AmpR promoter
     CDS             5045..5902
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6076..6664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca ctgatgaatc ccctaatgat tttggtaaaa
      181 atcattaagt taaggtggat acacatcttg tcatatgatc aaatggtttc gcgaaaaatc
      241 aataatcaga caacaagatg tgcgaactcg atattttaca cgactctctt taccaattct
      301 gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac gcattacttg
      361 actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa gcgagaacaa
      421 aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac aaagagcgac
      481 tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg cccattgtac
      541 ttgttgactg gtctgatatt cgtgagcaaa aacgacttat ggtattgcga gcttcagtcg
      601 cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca gagcaatgtt
      661 caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg agtaacacca
      721 caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc gttgagaagc
      781 tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac ctaggagcgg
      841 aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca aagactttag
      901 gctataagag gctgactaaa agcaatccaa tctcatgcca aattctattg tataaatctc
      961 gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac ccgtcaccta
     1021 aaatctactc agcgtcggca aaggagccat gggttctagc aactaactta cctgttgaaa
     1081 ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt gaagaaacct
     1141 tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga acgagcagct
     1201 cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca tgttggcttg
     1261 cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac acagtcagaa
     1321 atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat tctggctaca
     1381 caataacaag ggaagactta ctcgtggctg caaccctact agctcaaaat ttattcacac
     1441 atggttacgc tttggggaaa ttatgagggg atctctcagt gcaaagcatg catctcaatt
     1501 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     1561 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     1621 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     1681 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     1741 gcctaggctt ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaagctgca
     1801 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     1861 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     1921 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
     1981 acagctctta aggctagagt acttaatacg actcactata ggctagccac catgacttcg
     2041 aaagtttatg atccagaaca aaggaaacgg atgataactg gtccgcagtg gtgggccaga
     2101 tgtaaacaaa tgaatgttct tgattcattt attaattatt atgattcaga aaaacatgca
     2161 gaaaatgctg ttattttttt acatggtaac gcggcctctt cttatttatg gcgacatgtt
     2221 gtgccacata ttgagccagt agcgcggtgt attataccag accttattgg tatgggcaaa
     2281 tcaggcaaat ctggtaatgg ttcttatagg ttacttgatc attacaaata tcttactgca
     2341 tggtttgaac ttcttaattt accaaagaag atcatttttg tcggccatga ttggggtgct
     2401 tgtttggcat ttcattatag ctatgagcat caagataaga tcaaagcaat agttcacgct
     2461 gaaagtgtag tagatgtgat tgaatcatgg gatgaatggc ctgatattga agaagatatt
     2521 gcgttgatca aatctgaaga aggagaaaaa atggttttgg agaataactt cttcgtggaa
     2581 accatgttgc catcaaaaat catgagaaag ttagaaccag aagaatttgc agcatatctt
     2641 gaaccattca aagagaaagg tgaagttcgt cgtccaacat tatcatggcc tcgtgaaatc
     2701 ccgttagtaa aaggtggtaa acctgacgtt gtacaaattg ttaggaatta taatgcttat
     2761 ctacgtgcaa gtgatgattt accaaaaatg tttattgaat cggacccagg attcttttcc
     2821 aatgctattg ttgaaggtgc caagaagttt cctaatactg aatttgtcaa agtaaaaggt
     2881 cttcattttt cgcaagaaga tgcacctgat gaaatgggaa aatatatcaa atcgttcgtt
     2941 gagcgagttc tcaaaaatga acaaatgtcg acggatcctt caacttccct gagctcgaag
     3001 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg
     3061 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     3121 cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt
     3181 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     3241 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     3301 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc
     3361 ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat
     3421 taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     3481 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac
     3541 cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg
     3601 ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca
     3661 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     3721 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     3781 tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc
     3841 aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca
     3901 aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag
     3961 tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca
     4021 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     4081 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     4141 gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa
     4201 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     4261 tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta
     4321 aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca
     4381 acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     4441 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     4501 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     4561 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     4621 agaagggcgg aaagtccaaa ttgtaacacg tgtaattcta gagcggccgc ttcgagcaga
     4681 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4741 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4801 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4861 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccaggtgg
     4921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6721 catggctcga c
//
