LOCUS       62056_1975        5679 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          493..625
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        670..688
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             698..1630
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1942..3591
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="LEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     polyA_signal    complement(3634..3755)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3888..3992
                     /label=AmpR promoter
     CDS             3993..4850
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5024..5612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
       61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      661 tagagtactt aatacgactc actataggct agccaccatg acttcgaaag tttatgatcc
      721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
      781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
      841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
      901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
      961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
     1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
     1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
     1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
     1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
     1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
     1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
     1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
     1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
     1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
     1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
     1621 aaatgaacaa atgtcgacac caggatgctt atgttcttaa gtctatattt gcatatacat
     1681 tgactcttga tggaaagact taagaagatc aaggtctcac catttgtcct caattcgtgt
     1741 gaccataaga tactgatagc attgagtctt gaaatgattt aataatatga gtgaggattt
     1801 gctttctcca ttagagcatt aagctaaaac tatcaacatt ttaaaccaaa ttgccttatt
     1861 tttcttccaa acttcatata tgtctatcag gtaataatag gcttgaaaat tgatatcctg
     1921 tggtgctaaa gtacagtaga gctcgaagac gccaaaaaca taaagaaagg cccggcgcca
     1981 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
     2041 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
     2101 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     2161 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     2221 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     2281 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
     2341 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
     2401 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     2461 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
     2521 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
     2581 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     2641 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     2701 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     2761 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     2821 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     2881 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     2941 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     3001 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     3061 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     3121 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     3181 aaggatggat ggctacattc tggagacata gcttactggg acgaagacga acacttcttc
     3241 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     3301 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc
     3361 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     3421 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     3481 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     3541 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaacacgtg
     3601 taattctaga gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     3661 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3721 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3781 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     3841 gtggtaaaat cgataaggat ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3901 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3961 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4021 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4081 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4141 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4201 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4261 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4321 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4381 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4441 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4501 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4561 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4621 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4681 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4741 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4801 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4861 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5161 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5641 tcctggcctt ttgctggcct tttgctcaca tggctcgac
//
