LOCUS       40924_2533        7602 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway vector p2FLGW7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
  TITLE     Translational repression by a miniature inverted-repeat transposable
            element in the 3' untranslated region
  JOURNAL   Nat Commun 8, 14651 (2017)
  PUBMED    28256530
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Shen J, Liu J, Xie K, Xing F, Xiong F, Xiao J, Li X, Xiong L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2017) Huazhong Agricultural University, National 
            Key Laboratory of Crop Genetic Improvement, Shizishan Street No. 1, 
            Wuhan, Hubei 430070, China
REFERENCE   3  (bases 1 to 7602)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            8, 14651 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-JAN-2017) Huazhong Agricultural University, National Key 
            Laboratory of Crop Genetic Improvement, Shizishan Street No. 1, 
            Wuhan, Hubei 430070, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(25..43)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(50..66)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      207..662
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        740..844
                     /label=AmpR promoter
     CDS             845..1702
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1876..2464
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2752..2773
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2788..2818
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2826..2842
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2850..2866
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2884..2902
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        3562..3906
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3999..5648
                     /label=luciferase
                     /note="firefly luciferase"
     protein_bind    5658..5782
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5807..5837
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5891..6547
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6892..7194
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7238..7362)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      7372..7597
                     /label=35S terminator
                     /note="35S terminator"
                     /regulatory_class="terminator"
ORIGIN
        1 catgcgacgt cgggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
       61 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      121 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      181 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
      241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
      301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
      361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
      421 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
      481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
      541 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
      601 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
      661 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg
      721 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      781 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      841 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      901 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      961 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1021 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1081 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1141 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1201 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1261 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1321 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1381 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1441 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1501 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1561 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1621 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1681 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1741 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1801 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1861 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1921 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1981 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2041 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2101 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2161 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2221 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2281 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2341 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2401 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2461 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2521 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2581 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2641 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     2701 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2761 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2821 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2881 gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg ggagctctcc
     2941 catatggtcg actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt
     3001 tctctatctc tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac
     3061 caccgataat gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     3121 tagagaggcc tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga
     3181 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat
     3241 caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat
     3301 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt
     3361 tcctactgaa tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc
     3421 cgtgaagact ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat
     3481 cttcgtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     3541 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     3601 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
     3661 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
     3721 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
     3781 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     3841 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     3901 gaggactccg gtatttttac aacaatacca caacaaaaca aacaacaaac aacattacaa
     3961 tttactattc tagtcgacct gcaggcggcc gcactagtat ggaagacgcc aaaaacataa
     4021 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     4081 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     4141 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     4201 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     4261 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     4321 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     4381 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     4441 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     4501 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     4561 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     4621 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     4681 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     4741 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     4801 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     4861 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     4921 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     4981 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     5041 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     5101 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     5161 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     5221 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     5281 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     5341 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     5401 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     5461 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     5521 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     5581 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     5641 tcgccgtgta aggtaccaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     5701 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     5761 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     5821 ttccggctcg tataatgtgt ggattttgag ttaggatccg gcgagatttt caggagctaa
     5881 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     5941 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     6001 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     6061 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     6121 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     6181 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     6241 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     6301 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     6361 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     6421 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtctgtga
     6481 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     6541 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     6601 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     6661 aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     6721 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     6781 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     6841 ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga aatgcagttt
     6901 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     6961 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     7021 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     7081 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     7141 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     7201 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     7261 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     7321 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc
     7381 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     7441 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
     7501 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     7561 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca gg
//
