LOCUS       40924_2478        3204 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p2059-RS-nirA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Metabolically independent and accurately adjustable Aspergillus sp. 
            expression system
  JOURNAL   Appl. Environ. Microbiol. 71 (2), 672-678 (2005)
  PUBMED    15691916
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Pachlinger R, Mitterbauer R, Adam G, Strauss J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUN-2004) Department of Applied Plant Sciences and 
            Plant Biotechnology, BOKU - University of Natural Resources and 
            Applied Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria
REFERENCE   3  (bases 1 to 3204)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3204)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2005"; volume: "71"; issue: "2"; pages:
            "672-678"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUN-2004) Department of Applied Plant Sciences and Plant 
            Biotechnology, BOKU - University of Natural Resources and Applied 
            Life Sciences, Vienna, Muthgasse 18, Vienna A-1180, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(695..788)
                     /label=Aspergillus nidulans nirA promoter fragment
                     /note="Aspergillus nidulans nirA promoter fragment"
     misc_feature    complement(792..907)
                     /label=random sequence (RS) fragment derived from ampR
                     /note="random sequence (RS) fragment derived from ampR"
     misc_feature    complement(908..946)
                     /label=3xERE (estrogen responsive element)
                     /note="3xERE (estrogen responsive element)"
     primer_bind     complement(973..989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1019..1037)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1058..1074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1082..1098)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1106..1136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1151..1172)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1460..2048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2222..3079)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3080..3184)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atccatggta aatcaagccc agacagatat
      721 gttcggtgac agcgacagac caagacagcg catgcttatc ggaggaggac gctcggcgtg
      781 tttccctgca gaacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      901 acggaaaggt cactgtgacc ggtcactgtg accggtcact gtgaccagat ctgaattcga
      961 tatcaagctt atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc
     1021 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     1081 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1141 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1201 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1261 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1321 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1381 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1441 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1501 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1561 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1621 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1681 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1741 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1801 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1861 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     1921 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1981 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2041 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2101 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2161 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2221 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2281 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2341 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2401 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2461 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2521 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     2581 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     2641 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     2701 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     2761 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     2821 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     2881 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     2941 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3001 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3061 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3121 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3181 cgcgcacatt tccccgaaaa gtgc
//
