LOCUS       40924_2448        4013 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p1cVTm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Programmed hierarchical patterning of bacterial populations
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Boehm CR, Grant PK, Haseloff J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2017) Plant Sciences, University of Cambridge, 
            Downing Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom
REFERENCE   3  (bases 1 to 4013)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2017) Plant Sciences, University of Cambridge, Downing 
            Street, Cambridge, Cambridgeshire CB2 3EA, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(49..637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      818..852
                     /label=PJ23101*
                     /note="PJ23101*"
                     /regulatory_class="promoter"
     regulatory      861..878
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             861..872
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             879..1595
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     terminator      1618..1689
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1705..1732
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1748..2605)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2606..2710)
                     /label=AmpR promoter
     terminator      complement(2800..2843)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2846..2867
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     terminator      complement(2874..2901)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(2917..2988)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(3011..3727)
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     regulatory      complement(3728..3745)
                     /label=BBa_B0034
                     /note="BBa_B0034"
                     /regulatory_class="ribosome_binding_site"
     RBS             3734..3745
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      complement(3754..3788)
                     /label=PJ23101
                     /note="PJ23101"
                     /regulatory_class="promoter"
     misc_feature    3789..3809
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3810..3867
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
       61 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
      301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
      361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
      421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
      481 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
      541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
      601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
      661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
      721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      781 tatatatgag taaacttggt ctgacagttt actagagttt acagctagct cagtcctagg
      841 ttttatgcta gctactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagc
      901 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      961 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga
     1021 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg
     1081 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1141 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1201 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1261 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1321 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     1381 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc
     1441 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc
     1501 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1561 ccgggatcac tctcggcatg gacgagctgt acaagtaata atactagagc caggcatcaa
     1621 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1681 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     1741 ctagagacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1801 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1861 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1921 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1981 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2041 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2101 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2161 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2221 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2281 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2341 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2401 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2461 gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2521 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2581 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2641 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2701 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2761 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     2821 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagtat aaacgcagaa
     2881 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
     2941 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggctct
     3001 agtattatta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     3061 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     3121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3181 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
     3241 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtcgctaaag tagttgtact
     3301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     3361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     3481 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg cacgccccag gacagggtgg
     3541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     3601 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     3661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     3721 tcaccatcta gtatttctcc tctttctcta gtagctagca taatacctag gactgagcta
     3781 gctgtaaata ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     3841 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     3901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaa
//
