LOCUS       V011769                10723 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V011769
VERSION     V011769
KEYWORDS    p18sheLL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL,
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
   PUBMED   17603495
REFERENCE   2  (bases 1 to 10723)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10723)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med.";
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..16
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2229..3614
                     /gene="L2"
                     /label="Minor capsid protein L2"
                     /note="Minor capsid protein L2 from Human papillomavirus
                     type 18. Accession#: P06793"
     misc_feature    3659..4247
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     polyA_signal    4290..4514
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4560..4988
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4997..5017)
                     /label="pBABE 3'"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             6946..7311
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    7444..7577
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7614..7630)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(7614..7630)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7627..7649)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    7638..7654
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7662..7692)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7707..7728)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7845..7862)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8016..8601)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8775..9632)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9633..9737)
                     /label="AmpR promoter"
     primer_bind     complement(9812..9831)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        10003..10382
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10383..10586
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    10588..10606
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10609..10627
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10637..10661
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ccggtcgcca ccatggccct ctggagacca tccgataaca cagtgtactt gcccccaccc
       61 agcgtcgccc gggtggtgaa cacagacgac tacgtcacca gaacctcaat cttctaccac
      121 gccgggtcca gccggctgct gaccgtgggc aacccctact tccgcgtgcc cgccggcggc
      181 ggaaacaaac aagacatccc caaagtcagc gcctatcagt accgggtgtt ccgcgtccaa
      241 ctgcccgatc ccaacaagtt cggcctgccc gacacctcca tctacaaccc cgagacccag
      301 aggctggtct gggcttgcgc cggcgtcgag atcgggaggg gccaacccct gggcgtgggg
      361 ttgtccggcc accccttcta caacaagctg gacgataccg agtccagcca cgcagcaacc
      421 agcaacgtct ccgaagatgt gcgcgataac gtcagcgtgg actacaaaca aacccaactg
      481 tgcatcctgg gatgcgcacc cgccatcggc gagcattggg ccaaggggac cgcctgcaag
      541 agcaggcccc tgagccaagg ggactgtcca cccctggagt tgaagaatac cgtgctcgag
      601 gacggcgaca tggtggacac cggctacggc gctatggatt tctccaccct ccaggacacc
      661 aagtgcgaag tgcccctcga catctgccaa agcatctgca agtaccccga ctacctccag
      721 atgagcgccg acccctacgg cgacagcatg ttcttctgtc tcagaaggga acaattgttc
      781 gcccgccact tctggaaccg ggccggcaca atgggagata cagtccccca gagcctgtac
      841 atcaagggga ccggaatgag ggccagcccc gggtcctgcg tctacagccc aagcccctcc
      901 gggagcatcg tcacaagcga tagccaactc ttcaacaagc cctactggct ccacaaagcc
      961 caaggccaca ataacggggt gtgttggcac aaccagctgt tcgtgaccgt cgtggacaca
     1021 accaggtcca caaacctgac catctgcgcc agcacccaaa gccccgtgcc cggccagtac
     1081 gacgccacaa agttcaaaca atactctcgg cacgtggaag agtacgacct ccaattcatc
     1141 ttccaactct gcaccatcac cctcaccgcc gacgtgatga gctacatcca ctccatgaac
     1201 tcctccatcc tggaagactg gaatttcggc gtgccaccac cccctaccac ctccctcgtc
     1261 gacacctaca gattcgtgca gagcgtggcc atcacatgcc agaaagacgc cgcccccgcc
     1321 gagaacaaag acccatacga caaactgaaa ttctggaacg tcgacctgaa agagaaattc
     1381 agcctggatc tggaccagta cccattgggc aggaagttcc tcgtgcaagc cggcctcagg
     1441 agaaaaccaa caatcgggcc caggaagagg agcgccccca gcgcaaccac cagcagcaag
     1501 cccgcaaaaa gggtcagagt gagggcacgc aaatgagcta gaactagtgg atcccccggg
     1561 ctgcaggaat tcgatgggtc gacctgcaga agcttaaaac agctctgggg ttgtacccac
     1621 cccagaggcc cacgtggcgg ctagtactcc ggtattgcgg tacccttgta cgcctgtttt
     1681 atactccctt cccgtaactt agacgcacaa aaccaagttc aatagaaggg ggtacaaacc
     1741 agtaccacca cgaacaagca cttctgtttc cccggtgatg tcgtatagac tgcttgcgtg
     1801 gttgaaagcg acggatccgt tatccgctta tgtacttcga gaagcccagt accacctcgg
     1861 aatcttcgat gcgttgcgct cagcactcaa ccccagagtg tagcttaggc tgatgagtct
     1921 ggacatccct caccggtgac ggtggtccag gctgcgttgg cggcctacct atggctaacg
     1981 ccatgggacg ctagttgtga acaaggtgtg aagagcctat tgagctacat aagaatcctc
     2041 cggcccctga atgcggctaa tcccaacctc ggagcaggtg gtcacaaacc agtgattggc
     2101 ctgtcgtaac gcgcaagtcc gtggcggaac cgactacttt gggtgtccgt gtttcctttt
     2161 attttattgt ggctgcttat ggtgacaatc acagattgtt atcataaagc gaattggatt
     2221 gcggccgcat ggtcagccat agggctgcca ggaggaagag agccagcgtg accgatctgt
     2281 acaagacctg caagcagagc ggcacctgcc cccccgacgt ggtgcccaaa gtcgaaggga
     2341 caaccctggc cgacaagatc ctccagtgga gctctttggg catcttcctc ggcggcttgg
     2401 ggatcggcac cgggtccggc accggcggca ggaccggcta tatccccctc gggggcagga
     2461 gcaacaccgt cgtcgacgtg ggccccacca ggcctcccgt cgtgatcgag cccgtcgggc
     2521 ctaccgatcc cagcatcgtg accctgatcg aagatagctc cgtcgtgacc agcggcgccc
     2581 cccgccccac cttcaccggg accagcggct tcgacatcac cagcgccggc accaccaccc
     2641 ccgccgtgct cgacattacc cccagcagca caagcgtcag catcagtacc acaaacttca
     2701 caaaccccgc cttcagcgac cccagcatca tcgaggtgcc ccagaccggc gaagtcgccg
     2761 gcaacgtgtt cgtgggcaca cccaccagcg gcacccacgg ctacgaagag atccccctgc
     2821 agaccttcgc cagcagcggc accggcgaag agccaatctc ctccacaccc ctccccaccg
     2881 tcagaagggt ggccggccca aggttgtatt cccgcgctta tcagcaagtc agcgtcgcca
     2941 atcccgaatt cctgaccagg cccagcagcc tgatcaccta cgataacccc gctttcgaac
     3001 ccgtcgatac caccctgacc ttcgacccca ggtccgacgt gcccgacagc gacttcatgg
     3061 acatcattag gttgcaccgc cccgccctga ccagccgcag gggcaccgtg aggttctccc
     3121 ggctgggcca gagagccacc atgttcacaa ggtctggcac ccagatcggc gcccgcgtgc
     3181 atttctatca cgacatctcc cccatcgccc ccagccccga gtacatcgag ctccaacccc
     3241 tcgtgagcgc taccgaagat aacgatctct tcgacatcta cgccgacgat atggatcccg
     3301 ccgtccccgt gcccagcagg agcaccacaa gcttcgcctt cttcaagtac agcccaacca
     3361 tcagcagcgc tagcagttac tccaacgtga ccgtgcccct gacaagtagc tgggacgtcc
     3421 ccgtgtatac cggccccgac atcaccctgc ccagcaccac aagcgtgtgg ccaatcgtga
     3481 gcccaaccgc tcccgctagc acccaataca tcggcatcca cggcacccac tactacctct
     3541 ggcccctgta ctacttcatc cccaagaaga ggaagagggt gccatacttc ttcgccgacg
     3601 ggttcgtcgc cgcttgagat cccccgggct gcaggaattc gatatcaagc ttatcgataa
     3661 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3721 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3781 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3841 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3901 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3961 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4021 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4081 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4141 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4201 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     4261 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4321 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4381 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4441 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4501 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4981 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5041 gcaggcagaa gtatgcaaag catgcagaat tctatcaaat atttaaagaa aaaaaaattg
     5101 tatcaacttt ctacaatctc tttcagaaga cagaagcaga gggaatactt cctaaatcat
     5161 tcaactaggc cagcattacc ttaataccgg aactagaaaa tgacattaca agaaaagaaa
     5221 acaacagacc aatatctctc atgaacaaag atacaaacat tttcaacaaa atattagcaa
     5281 aaagaatcca agaatgtatc aaaaaatata caccacaacc aagtagaatt tattccagat
     5341 atgtaagggt ggttcaacgt ttgaaaatca attaacgtaa tttgtcccat caacaggtta
     5401 aagaagaaaa tcacatggtc atattgatag acacagaaaa agcatttgac aaaatttaac
     5461 acccattcat gatgcaatct ctcagtaaac taggaataga ggaaaacttc ctcagcttga
     5521 atgtaccttc ctctcaattt tgctatgaac ctgaaactcc tcttaaaaaa taaagttttt
     5581 catttaaaaa gaaaacaaaa aacatggagg agcgttgatg tatctcattt tagaccaatc
     5641 agctatggat agttaggcga cagcacagat agctgctgta cttctgtttc tggcaatgtt
     5701 ccagactaca tttaaaaaat ttttaattat agacttgtac ttaatgttca agaaaaatat
     5761 gaaaatggct ttgccgtgtt aatgctactc ttttttaaaa aaaactaaag ttcaaacttt
     5821 atttatattt cattagtttt ttagctactg ttctttttct gttctgggat ctcattcaga
     5881 atgccacatt acatataatt ctcatgtctc cttgggttcc tcttagtttt gacagttcct
     5941 cagacttttc ttatttttga tgaccttgac agttttgagg agtactggtt agatataggg
     6001 taatggtttt taaagtatat ttgtcatgat ttatactggg gtaagggttt ggggaggaag
     6061 cccatggggt aaagtactgt tctcatcaca tcatatcaag gttatatacc atcaatattg
     6121 ccacagatgt tacttagcct tttaatattt ctctaattta gtgtatatgc aatgatagtt
     6181 ctctgatttc tgagattgag tttctcatgt gtaatgatta tttagagttt ctctttcatc
     6241 tgttcaaatt tttgtctagt tttatttttt actgatttgt aagacttctt tttataatct
     6301 gcatattaca attctcttta ctggggtgtt gcaaatattt tctgtcattc tatggcctga
     6361 cttttcttaa tggtttttta attttaaaaa taagtcttaa tattcatgca atctaattaa
     6421 caatcttttc tttgtggtta ggactttgag tcataagaaa tttttctcta cactgaagtc
     6481 atgatggcat gcttctatat tattttctaa aagatttaaa gttttgcctt ctccatttag
     6541 acttataatt cactggaatt tttttgtgtg tatggtatga catatgggtt cccttttatt
     6601 ttttacatat aaatatattt ccctgttttt ctaaaaaaga aaaagatcat cattttccca
     6661 ttgtaaaatg ccatattttt ttcataggtc acttacatat atcaatgggt ctgtttctga
     6721 gctctactct attttatcag cctcactgtc tatccccaca catctcatgc tttgctctaa
     6781 atcttgatat ttagtggaac attctttccc attttgttct acaagaatat ttttgttatt
     6841 gtcttttggg cttctatata cattttagaa tgaggttggc aagttaacaa acagcttttt
     6901 tggggtgaac atattgacta caaatttatg tggaaagaaa gtaccaagtt gaccagtgcc
     6961 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     7021 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     7081 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     7141 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     7201 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     7261 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     7321 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7381 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7441 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7501 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7561 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7621 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7681 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7741 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7801 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8521 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
     8581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8641 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8701 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8761 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8821 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8881 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8941 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9001 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9061 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9121 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9181 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9241 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9301 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9361 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9421 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9481 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9541 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9601 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9661 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9721 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     9781 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9841 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     9901 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     9961 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
    10021 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
    10081 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
    10141 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
    10201 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
    10261 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
    10321 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
    10381 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
    10441 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggaaccaa aatcaacggg
    10501 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
    10561 ggtgggaggt ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg
    10621 atagagatcg tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac
    10681 gctgttttga cctccataga agacaccggg accgatccag cct
//
