LOCUS       62056_1880        5337 bp DNA     circular SYN 31-OCT-2021
DEFINITION  Expression vector p15Tc-litR, complete sequence.
ACCESSION   MZ683493
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Bazhenov S, Melkina O, Fomin V, Scheglova E, Krasnik P, Khrulnova S,
            Zavilgelsky G, Manukhov I.
  TITLE     LitR directly upregulates autoinducer synthesis and luminescence in 
            Aliivibrio logei
  JOURNAL   PeerJ 9, e12030 (2021)
  PUBMED    34616599
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Bazhenov SV, Manukhov IV.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2021) Laboratory for Molecular Genetics, Moscow 
            Institute of Physics and Technology, Pervomayskaya, 3, Dolgoprudny, 
            Moscow Region 141701, Russia
REFERENCE   3  (bases 1 to 5337)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ 9, 
            e12030 (2021)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-AUG-2021) Laboratory for Molecular Genetics, Moscow Institute of
            Physics and Technology, Pervomayskaya, 3, Dolgoprudny, Moscow Region
            141701, Russia"
FEATURES             Location/Qualifiers
     source          1..5337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..39
                     /label=Ptac sequencing primer
                     /note="Ptac sequencing primer"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     gene            258..863
                     /gene="litR"
                     /label=litR
                     /note="derived from Aliivibrio logei"
     CDS             258..863
                     /codon_start=1
                     /transl_table=11
                     /gene="litR"
                     /product="LitR family transcriptional regulator"
                     /label=litR
                     /protein_id="UDM84365.1"
                     /translation="METIQKRPRTRLSPEKRKEQLLDIAIEVFSQRGIGRGGHADIAEI
                     AQVSVATVFNYFPTREDLVDDVLNRVEEQFHNFILNSISLDRDVRSNLHSLLVNIIDTV
                     QMDNKWIKVWFEWSTSTREEVWPLFLSTQVNNQKVIHDMFAEGIERNEVCNDHTPENLA
                     KMLHGICYSVFIQANRNSSYEELEQTANCFLNMLCIYK"
     rep_origin      complement(1278..1823)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1935..1963
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2011..3198
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        3608..3685
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3686..4765
                     /label=lacI
                     /note="lac repressor"
     protein_bind    4781..4802
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4817..4847
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             4891..5064
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agaattcatg gaaacgatcc aaaaaagacc gagaactagg ctatctccag
      301 agaaacgaaa agaacaacta cttgatattg cgattgaggt tttttctcaa cgtggtattg
      361 gtcgtggcgg tcatgcagat atagcagaaa ttgctcaagt atctgttgct acggtattta
      421 attacttccc aacccgagaa gatctggttg atgatgtatt aaatcgagtt gaagaacaat
      481 ttcataactt tattctaaat tcaatctcat tagaccgaga tgtacgctct aatcttcact
      541 ctttactcgt gaacatcata gatacagttc aaatggataa taagtggatt aaagtgtggt
      601 ttgaatggag cacctctact cgcgaagaag tatggcctct tttcttaagc actcaagtga
      661 ataaccagaa agttattcat gatatgtttg ctgaagggat tgagcgtaac gaagtatgta
      721 atgatcatac ccctgaaaac ttagctaaaa tgcttcatgg catttgttac tctgtattta
      781 ttcaagcaaa ccgtaatagc tcttatgaag aactagaaca aacggcaaac tgcttcttaa
      841 atatgctgtg tatttataaa taatggtacc cgggtgatgc tgccaactta ctgatttagt
      901 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
      961 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg
     1021 agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg
     1081 caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc
     1141 ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta
     1201 cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg
     1261 gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga
     1321 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1381 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     1441 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     1501 caagctggac tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa
     1561 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg
     1621 taattgattt agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga
     1681 caagttttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc
     1741 agagaacctt cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta
     1801 cgcgcagacc aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga
     1861 tttcagtgca atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt
     1921 tgtaattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag
     1981 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
     2041 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
     2101 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
     2161 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
     2221 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
     2281 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
     2341 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     2401 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     2461 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     2521 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     2581 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     2641 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     2701 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     2761 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
     2821 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
     2881 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
     2941 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
     3001 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact
     3061 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
     3121 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     3181 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc
     3241 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc
     3301 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg
     3361 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     3421 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     3481 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     3541 tttcgtaaag tctggaaacg cggaagtcgt aaagtctgga aacgcggaag cacaccgcat
     3601 aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
     3661 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     3721 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     3781 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     3841 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     3901 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     3961 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     4021 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     4081 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     4141 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     4201 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     4261 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
     4321 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
     4381 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
     4441 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
     4501 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
     4561 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
     4621 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     4681 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     4741 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     4801 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4861 gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc actggccgtc
     4921 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4981 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     5041 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
     5101 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
     5161 tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc
     5221 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
     5281 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt tggaatt
//
