LOCUS       62056_1875        3947 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector p15A-RBS1-A118, complete sequence.
ACCESSION   MN623121
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..3947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     gene            730..2088
                     /gene="a118"
                     /label=a118
     CDS             730..2088
                     /codon_start=1
                     /transl_table=11
                     /gene="a118"
                     /product="A118 serine recombinase"
                     /label=a118
                     /note="A118"
                     /protein_id="QJR97832.1"
                     /translation="MKAAIYIRVSTQEQVENYSIQAQTEKLTGLCRSKDWDVYDIFIDG
                     GYSGSNMNRPALNEMLSKLHEIDAVVVYRLDRLSRSQRDTITLIEEYFLKNNVEFVSLS
                     ETLDTSSPFGRAMIGILSVFAQLERETIRDRMVMGKIKRIEAGLPLTTAKGRTFGYDVI
                     DTKLYINEEEAKQLQLIYDIFEEEQSITFLQKRLKKLGFKVRTYNRYNNWLTNDLYCGY
                     VSYKDKVHVKGIHEPIISEEQFYRVQEIFTRMGKNPNMNRDSASLLNNLVVCSKCGLGF
                     VHRRKDTMSRGKKYHYRYYSCKTYKHTHELEKCGNKIWRADKLEELIINRVNNYSFASR
                     NVDKEDELDSLNEKLKIEHAKKKRLFDLYINGSYEVSELDSMMNDIDAQINYYESQIEA
                     NEELKKNKKIQENLADLATVDFDSLEFREKQLYLKSLINKIYIDGEQVTIEWL"
     terminator      2112..2183
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2199..2226
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2388..2933)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3047..3141)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3165..3821)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3822..3924)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aatttcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcagggacag
      721 gatggtacca tgaaggcagc tatttatata cgtgtttcta ctcaagagca agtagaaaat
      781 tattcaatac aagctcaaac tgaaaaacta acaggattgt gccgctcgaa ggactgggac
      841 gtatacgata ttttcattga cggcggatac tccggctcaa atatgaatcg tcccgcatta
      901 aatgaaatgc taagtaaact acacgaaatt gatgctgtag tcgtatatcg attagacaga
      961 ctatcccgct cacaaagaga cacaataacg cttattgaag aatacttctt aaaaaacaat
     1021 gtagagtttg ttagtttgtc tgaaacgctt gatactagtt cccctttcgg tcgtgcaatg
     1081 attggtatat tatcagtatt cgcacagcta gagcgcgaaa caatccgaga tcgtatggtg
     1141 atggggaaaa ttaagcgtat tgaagcaggt cttccgttaa caactgcgaa aggtagaacg
     1201 ttcggctatg atgttataga tacaaaatta tacattaatg aagaagaagc aaaacagtta
     1261 caactgattt atgatatttt cgaagaagaa caaagtataa cttttttaca gaaaagacta
     1321 aaaaaattag gctttaaagt tagaacatat aatcgctata acaactggct aactaatgat
     1381 ttgtattgtg gttatgtttc atataaagat aaagttcatg taaaaggtat tcatgaacct
     1441 atcatcagtg aagagcaatt ctatagagtt caagaaatat ttactcgtat gggtaaaaat
     1501 ccgaacatga atagagattc agcatcgttg ctaaataatt tagtagtttg tagtaaatgc
     1561 gggttaggct ttgttcatcg tagaaaagat acaatgtcgc gtggtaaaaa atatcattat
     1621 agatattata gttgcaagac ttataaacat actcatgaac tcgaaaaatg cgggaataaa
     1681 atttggagag ctgacaaact tgaagaatta attattaatc gtgtgaataa ttatagtttc
     1741 gcttccagaa atgtagataa agaagatgaa ttagatagct taaatgaaaa gcttaaaata
     1801 gaacatgcaa agaaaaaacg attatttgat ttatatataa atggctcgta tgaagtttca
     1861 gaacttgatt ctatgatgaa tgatattgat gctcaaatta attattatga atcacaaata
     1921 gaagctaacg aagaattgaa gaaaaacaaa aagatacaag aaaatttagc tgatttagca
     1981 acagttgatt ttgactcttt agagttcaga gaaaagcaac tttatttaaa atcactaata
     2041 aacaaaattt atattgatgg tgaacaagtt actattgaat ggctctagct cgagtaagga
     2101 tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2161 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2221 tctgcgttta tacctaggga tatattccgc ttcctcgctc actgactcgc tacgctcggt
     2281 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
     2341 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
     2401 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     2461 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     2521 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     2581 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     2641 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     2701 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     2761 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     2821 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     2881 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     2941 atcttattaa tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag
     3001 cacctgaagt cagccccata cgatataagt tgttactagt gcttggattc tcaccaataa
     3061 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     3121 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     3181 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     3241 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     3301 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     3361 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     3421 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     3481 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     3541 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     3601 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     3661 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     3721 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     3781 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     3841 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     3901 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatc
//
