LOCUS       V013889                 3787 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013889
VERSION     V013889
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(260..805)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(936..1559)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     promoter        1569..1624
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     RBS             1633..1655
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1656..2474
                     /gene="dacA"
                     /label="Diadenylate cyclase"
                     /note="Diadenylate cyclase from Listeria monocytogenes
                     serotype 1/2a (strain 10403S). Accession#: A0A0H3GM48"
     CDS             complement(2658..3464)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
       61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
      121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
      181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
      241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
      301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
      481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
      601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
      661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
      721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
      781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa aatatttcta
      841 gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca tacgatataa
      901 gttgtaattc tgccttttta cggttcctgg ccttaagacc cactttcaca tttaagttgt
      961 ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg
     1021 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
     1081 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
     1141 tgccccactg cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
     1201 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
     1261 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
     1321 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
     1381 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
     1441 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
     1501 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaaacg agacatcatt
     1561 tcctaattgt tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag
     1621 agaatagttc gatttgttta actttaagaa ggagaatgga cttttccaac atgagtatcc
     1681 ttcactattt agcgaatatt gtagacattt tggtggtttg gttcgtaatt tacaaggtta
     1741 tcatgcttat ccgcggcacg aaagccgtcc agctgttgaa ggggattttc attattattg
     1801 ccgtcaagtt acttagcggc ttcttcggat tgcagacagt ggaatggatt actgatcaaa
     1861 tgctgacttg gggtttctta gccattatca ttatctttca accggaattg cgtcgtgccc
     1921 tggagacttt ggggcgtggc aatatcttta cccgctatgg atcacgcatt gagcgtgaac
     1981 aacaccacct tattgagtct attgaaaagt ccacgcaata catggcgaag cgtcgcattg
     2041 gagctttgat ctctgtggct cgtgatacag gcatggacga ctacatcgag actggcattc
     2101 cgcttaatgc gaaaatctca tcgcaattat tgattaatat cttcatcccc aatacccctc
     2161 ttcacgatgg cgcagtgatc attaaaggta acgagatcgc gagcgctgcc agttatctgc
     2221 cattgtccga ctcgccgttt ctttctaagg agctgggaac tcgccatcgt gccgcattag
     2281 ggatttccga ggtgacagat tcaatcacaa tcgttgtcag cgaggaaaca ggtgggattt
     2341 cccttacgaa gggaggcgaa ctgttccgtg atgtatccga agaagaattg cataagattt
     2401 tgcttaaaga gctggtgact gtcacagcta aaaaaccaag cattttctcc aaatggaaag
     2461 gaggtaagtc cgagtgagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2581 tccttttaaa ttaaaaatga agttttaaat caagcccaat ctgaataatg ttacaaccaa
     2641 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     2701 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     2761 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     2821 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     2881 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     2941 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     3001 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     3061 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     3121 cctgaatcag gatattcttc taatacctgg aatgctgttt ttccggggat cgcagtggtg
     3181 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     3241 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     3301 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaagcgata gattgtcgca
     3361 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     3421 gaatttaatc gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     3481 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     3541 taacatcaga gattttgaga cacgggccag agctgcatcg cgcgtttcgg tgatgacggt
     3601 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     3661 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     3721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     3781 cacagat
//
