LOCUS       62056_1860        3744 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector p15A-HNS-GFP, complete sequence.
ACCESSION   MN623123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..3744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(10..633)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        649..704
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     gene            737..1147
                     /gene="hns"
                     /label=hns
     CDS             737..1147
                     /codon_start=1
                     /transl_table=11
                     /gene="hns"
                     /product="histone-like nucleoid structuring protein"
                     /label=hns
                     /note="HNS"
                     /protein_id="QJR97840.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEES
                     AAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDENG
                     ETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     CDS             1166..1882
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      1909..1980
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1996..2023
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2185..2730)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2844..2938)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2962..3618)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3619..3721)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aatttcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcgtcgacaa
      721 agaggagaaa gatatcatga gcgaagcact taaaattctg aacaacatcc gtactcttcg
      781 tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa aattagaagt
      841 tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg aagagcgcac
      901 tcgtaaactg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc cgaacgaact
      961 gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc agcgtccggc
     1021 aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc aaggccgtac
     1081 tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg atttcctgat
     1141 caagcaaact agtagatctg gtaccatggt gagcaagggc gaggagctgt tcaccggggt
     1201 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1261 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1321 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1381 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1441 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1501 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1561 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1621 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1681 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1741 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1801 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1861 cggcatggac gagctgtaca agtaactcga gtaaggatct ccaggcatca aataaaacga
     1921 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1981 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatac ctagggatat
     2041 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
     2101 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
     2161 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     2221 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2281 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     2341 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     2401 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     2461 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     2521 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     2581 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     2641 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     2701 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     2761 ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     2821 tataagttgt tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     2881 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     2941 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
     3001 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     3061 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     3121 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     3181 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     3241 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     3301 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     3361 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     3421 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     3481 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     3541 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     3601 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     3661 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     3721 aacgtctcat tttcgccaga tatc
//
