LOCUS       V015269                 8124 bp    DNA     circular SYN 11-FEB-2020
DEFINITION  Exported.
ACCESSION   V015269
VERSION     V015269
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Swift RP, Rajaram K, Liu HB, Dziedzic A, Jedlicka AE, Roberts AD,
            Matthews KA, Jhun H, Bumpus NN, Tewari SG, Wallqvist A, Prigge ST.
  TITLE     A mevalonate bypass system facilitates elucidation of plastid
            biology in malaria parasites
  JOURNAL   PLoS Pathog. (2020) In press
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Prigge ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2019) Molecular Microbiology and Immunology, Johns
            Hopkins SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore,
            MD 21205, USA
REFERENCE   3  (bases 1 to 8124)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-DEC-2019) Molecular Microbiology and Immunology, Johns Hopkins
            SPH, 615 N. Wolfe, rm E5132, Johns Hopkins SPH, Baltimore, MD 21205,
            USA"
FEATURES             Location/Qualifiers
     source          1..8124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     35..84
                     /label="AttP"
                     /note="AttP"
     3'UTR           91..919
                     /label="HSP86 3'"
                     /note="HSP86 3'"
     CDS             complement(941..967)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             complement(974..1690)
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     mat_peptide     complement(1697..1864)
                     /gene="api-SFG-HA"
                     /product="HA-tagged Super Folder Green with an N-terminal
                     apicoplast trafficking peptide"
                     /note="Api55"
     5'UTR           1871..2837
                     /label="Pcam/Psti"
                     /note="Pcam/Psti"
     misc_feature    2844..3719
                     /gene="Mev"
                     /note="Region: Chimeric lower mevalonate pathway fusion
                     protein"
     CDS             3741..4316
                     /gene="PMVK"
                     /label="Phosphomevalonate kinase"
                     /note="Phosphomevalonate kinase from Homo sapiens.
                     Accession#: Q15126"
     CDS             4338..4883
                     /gene="idi"
                     /label="Isopentenyl-diphosphate Delta-isomerase"
                     /note="Isopentenyl-diphosphate Delta-isomerase from
                     Escherichia coli (strain K12 / DH10B). Accession#: B1XEH6"
     misc_feature    4905..5855
                     /gene="Mev"
                     /note="Region: Chimeric lower mevalonate pathway fusion
                     protein"
     3'UTR           5907..6469
                     /label="HRP2 3'"
                     /note="HRP2 3'"
     rep_origin      6594..7139
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        7183..7285
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             7286..7942
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 gcagtgtgga attccctgca ggcgcgcctc tagagggttt gtaccgtaca ccactgagac
       61 cgcggtggtt gaccagacaa accagcatgc tatttgatga attaactaca cttaaaataa
      121 tacaattatt attaaatttt tttttgattt atttattaat ttttaaactt aatcatttgt
      181 atttgggagg aattatatat atctttataa ttattttatt tttttttatt tttttatttt
      241 tttattatta ttattttttt ttattttttt tttttactgt atcaaagaaa aacctttaaa
      301 aaaaaaatta taatttcccc atcttactat atttttaata catacgtttt aaggaattaa
      361 attagacaaa agctatatta tgctttacat ataattagaa tttataaacg tttggttatt
      421 agatatttca tgtctcagta aagtctttca atacatatgt aaaaaaatat atatgaatac
      481 acataagttg ttaatatatt ttatatgcat aaatgtataa atatatatat atatatatat
      541 atatgtatgt atgtatatgt gtgtatatga aattatttca atgtttaatt ttttaaattt
      601 taattttttt tttttttttt ttttttatta tgtatattga tctttattat ttaaatatta
      661 cttttttcgt tttttcttct ttttattatt tttttttttt tttatatttt atacaaatgg
      721 taattcaaat aaaaggtata aatttatatt taattttctt ttatggataa ataaaagaaa
      781 aatataaata tataaaaata taaaaatata tatatgtata ttggggtgat gataaaatga
      841 aagataatat atatatatat atatctttat tttttttttt ttgtagaccc cattgtgagt
      901 acataaatat attatataac tcgaccttaa gctcgagtta tgcataatca ggcacatcgt
      961 atggatagct agctttatat aattcatcca ttccatgtgt aattcctgca gccgttacaa
     1021 attctagcaa aaccatgtga tctctttttt catttggatc ttttgacaaa acggattgcg
     1081 ttgataagta atgattatca ggtaataata ctggaccatc tcctattggt gtattttgtt
     1141 gataatgatc agctaattga actgatccat cctctacatt atgtcttatt ttaaaattag
     1201 ctttaattcc atttttttgt ttatctgctg ttatatacac attgtgacta ttaaaattat
     1261 attctaattt gtgaccaagt atattaccat cttccttaaa atcaatgccc tttaactcaa
     1321 ttctatttac taaagtatca ccttcaaact tgacttcagc tctcgtttta tatgttccat
     1381 catctttaaa tgaaattgtt ctttcctgaa catatccttc tggcattgca gatttaaaaa
     1441 aatcatgtct tttcatgtga tccggatatc gactaaaaca ttgtactccg taagttaagg
     1501 tcgttacaag tgttggccat ggaaccggta actttcctgt ggtacaaata aattttaatg
     1561 ttaattttcc atttgttgca tctccttcgc cttcaccacg tacagaaaat ttatggccat
     1621 tcacatctcc atccaattca actaatattg gaactacacc cgtaaataat tcctcacctt
     1681 ttgataccat actagtatgt gggtttttat tttttatcaa attgtaatca ttttttctat
     1741 ttaaaaaatt cacttggttg cttcttttct tttttaatat ttgtaaggac actccatctt
     1801 tttgtgtatt tttaaaagcg ttaacataat atagaaaaat tatacaaagt aataagatct
     1861 tcatcctagg ttgtaaaaaa aattaaaata tatttatata atattatttt attttattat
     1921 atattatatt atttttattt ttatttttat ttttttttct ctacaaattt tatctattgg
     1981 tttattataa aaatatctat ttctaataat aaataattaa gatatcaatt tatagaaaca
     2041 aaatatatac ttgtataatt ttattttttt atataaatca ttacatatat aattatacaa
     2101 tattttttct aagagataat tatatattaa tatatataaa aaaaggtgtt tttttttttt
     2161 atttttattt ttattttatg gtaatatttt attttcctta ttgtataaat tatattagtt
     2221 tatatgtgat taattttata tattatcaat ttatatattt ttaaatgctt acttaattat
     2281 cttttttttt tttttttttt tttttccttc tttttatatt tatttaattt ttgaaaaaat
     2341 tgatatatat atatatatat aatatatata tacatgtagt agtattaaac aatgtataat
     2401 atatataaat aatatattta tatatttcat ttcaatttta atttttcttg tttttttttt
     2461 ttctttttgt catatttaaa aaaaattata ttcatataag ttatgcattt tttataaaca
     2521 ttattcaata tatgtataat ataatatata tatatatatt aatgtattat tccaatgtgc
     2581 atgataaaag aaaaaaataa tatttataaa aaaaaagaaa aataaaacaa aaaaagaaaa
     2641 aaaaaaaaaa aaaaaaaaat acaaaaataa ataatataat ttataattat atattcttgt
     2701 cacaataaaa atatatatat atatatatat atttataata tgtatatttt aaactagaaa
     2761 aggaataact aatattttat ttattatcat tcaagattta tattttataa taataaatac
     2821 ctaatagaaa tatatcagga tccatgacca aaaaagttgg tgttggtcag gcacatagca
     2881 aaattatctt gattggtgaa catgccgtgg tttatggtta tccggcaatt agcttaccgt
     2941 tgttagaagt tgaagttacc tgtaaagttg ttccggcaga aagcccgtgg cgtttatatg
     3001 aagaggatac cttgagcatg gcagtttatg caagtttaga atatttgaat atcaccgaag
     3061 catgtattcg ttgcgaaatt gatagcgcaa tcccggaaaa acgtggtatg ggtagcagcg
     3121 cagcaattag cattgcagca attcgtgcag tgttcgatta ttatcaggcc gatttaccgc
     3181 atgatgtttt ggaaatttta gttaatcgtg cagaaatgat tgcacacatg aatccgagcg
     3241 gtttggatgc aaaaacctgt ttaagcgatc agccgattcg ttttatcaaa aatgtgggtt
     3301 ttaccgaatt ggaaatggat ttaagcgcat atttggttat tgcagatacc ggtgtttatg
     3361 gtcatacccg tgaagcaatt caggttgttc agaataaagg taaagatgca ttaccgtttt
     3421 tgcatgcatt aggtgaattg acccagcagg cagaaattgc cattagccag aaagatgcag
     3481 aaggtttagg tcagattttg agccaggcac atttacattt gaaagaaatt ggtgttagca
     3541 gcttagaagc agatagcttg gttgaaaccg cattaagtca tggtgccttg ggtgcaaaaa
     3601 tgagcggtgg tggtttaggt ggttgtatta ttgcattggt taccaattta acacatgcac
     3661 aagaattggc agaacgttta gaagaaaaag gtgccgttca gacctggatt gaaagcctgg
     3721 gatctggttc tggaactagt atggcaccgt tgggtggcgc accgcgttta gttttgttat
     3781 ttagcggtaa acgtaaaagc ggtaaagatt ttgttaccga agcattacag agccgtttgg
     3841 gtgccgatgt ttgtgcagtt ttacgtttga gcggtccgtt aaaagaacag tatgcccaag
     3901 aacatggttt gaattttcag cgtttattgg ataccagcac ctataaagaa gcatttcgta
     3961 aagatatgat tcgttggggt gaagaaaaac gtcaggcaga tccgggtttt ttttgccgta
     4021 aaattgttga aggtattagc cagccgattt ggttagttag cgatacccgt cgtgtgagcg
     4081 atattcagtg gtttcgtgaa gcctatggtg cagttaccca gaccgttcgt gttgttgcat
     4141 tggaacagag tcgtcagcag cgtggttggg tttttacacc gggtgttgat gatgcagaaa
     4201 gcgaatgtgg tttagataac tttggtgatt ttgattgggt gatcgaaaat catggtgttg
     4261 aacagcgttt ggaagaacag ttagaaaatt tgattgaatt tattcgtagt cgtctgggta
     4321 ccggaagtgg tcgtacgatg cagaccgaac atgttatttt gttaaatgca cagggtgttc
     4381 cgaccggcac cttggaaaaa tatgcagcac ataccgcaga tacccgttta catttggcat
     4441 ttagcagctg gttatttaat gcaaaaggtc agttgttagt tacccgtcgt gccttgagca
     4501 aaaaagcatg gcctggtgtt tggaccaata gcgtttgtgg tcatccgcag ttaggtgaaa
     4561 gcaatgaaga tgcagttatt cgtcgttgtc gttatgaatt gggtgttgaa attaccccac
     4621 cggaaagcat ttatcctgat tttcgttatc gtgcaaccga tccgagcggt attgttgaaa
     4681 atgaagtttg tccggttttt gcagcacgta ccacctcagc attacagatt aatgatgatg
     4741 aagtgatgga ttatcagtgg tgtgatttgg cagatgtttt acatggtatt gatgcaaccc
     4801 cgtgggcctt tagcccgtgg atggttatgc aggcaaccaa tcgtgaagca cgtaaacgtt
     4861 tgagcgcatt tacccagtta aaatccggaa gtggttcagg gcccatggat cgtgaaccgg
     4921 ttaccgttcg tagctatgca aatattgcca tcatcaaata ttggggcaaa aaaaaagaaa
     4981 aagagatggt tccggcaacg agcagcatta gcttgacatt agaaaatatg tataccgaaa
     5041 ccaccttgag tccgttacct gcaaatgtga ccgcagatga attttacatt aatggccagt
     5101 tgcagaacga agttgaacat gcaaaaatga gcaaaatcat cgatcgttat cgtcctgccg
     5161 gtgaaggttt tgttcgtatt gatacccaga ataatatgcc gaccgcagca ggtttaagca
     5221 gcagcagtag cggtttgtca gcattagtta aagcatgtaa tgcatatttc aaattgggtt
     5281 tagatcgtag tcagttggca caagaagcaa aatttgcaag cggtagcagc agccgttcat
     5341 tttatggtcc gttaggtgca tgggataaag atagcggtga aatttatccg gttgaaaccg
     5401 atttgaaatt agcaatgatt atgttggttt tagaagataa aaaaaaaccg attagcagcc
     5461 gtgatggtat gaaattgtgc gttgaaacca gcaccacctt tgatgattgg gttcgtcaga
     5521 gcgaaaaaga ttatcaggat atgttaatct atttgaaaga gaacgatttc gccaaaattg
     5581 gtgaattaac cgaaaaaaat gccttggcaa tgcatgcaac caccaaaacc gcaagtccgg
     5641 catttagtta tttaaccgat gcaagctatg aagccatgga ttttgtgcgt cagttgcgtg
     5701 aaaaaggtga agcatgttat tttaccatgg atgcaggtcc gaatgtgaaa gttttttgtc
     5761 aagagaaaga cttagaacac ttgagcgaaa tttttggtca gcgttatcgt ttaattgtga
     5821 gcaaaaccaa agacttgagc caggatgatt gttgtgctag caatgctaac cctaacgcaa
     5881 atccaaatgc aaacccttaa aagcttattt aataatagat taaaaatatt ataaaaataa
     5941 aaacataaac acagaaatta caaaaaaaat acatatgaat tttttttttg taatcttcct
     6001 tataaatata gaataatgaa tcatataaaa catatcatta ttcatttatt tacatttaaa
     6061 attattgttt cagtatcttt aatttattat gtatatataa aaataactta caattttatt
     6121 aataaacaat atatgtttat taattcatgt tttgtaattt atgggatagc gatttttttt
     6181 actgtctgta tttttctttt ttaattatgt tttaattgta ttttattttt attattgttc
     6241 tttttatagt attattttaa aacaaaatgt attttctaag aacttataat aataataaat
     6301 ataaatttta ataaaaatta tatttatctt ttacaatatg aacataaagt acaacattaa
     6361 tatatagctt ttaatatttt tattcctaat catgtaaatc ttaaattttt ctttttaaac
     6421 atatgttaaa tatttatttc tcattatata taagaacata tttattaaat ctaggctggc
     6481 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     6541 cgagctcact agtagatcta gaaatatttt atctgattaa taagatgatc ttcttgagat
     6601 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt
     6661 gtttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg
     6721 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
     6781 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
     6841 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
     6901 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
     6961 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
     7021 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7081 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     7141 acggctttgc cgcgggctag ccccggggcg gccgcgagac gttgatcggc acgtaagagg
     7201 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     7261 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     7321 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     7381 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     7441 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgca
     7501 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     7561 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     7621 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     7681 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     7741 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     7801 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     7861 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     7921 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     7981 tggtgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat
     8041 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt
     8101 ttaccggttt attgactacc ggaa
//
