LOCUS       40924_2423        9527 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p1300-amiRNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Li Y, Li Y, Zhao S, Zhong S, Wang Z, Ding B, Li Y.
  TITLE     A simple method for construction of artificial microRNA vector in 
            plant
  JOURNAL   Biotechnol. Lett. (2014) In press
  PUBMED    24930106
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   Li Y, Li Y, Zhao SP, Lu N.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2013) Wuhan University, College of Life Sciences, 
            Luo Jia Shan, Wuhan, Hubei 430072, China
REFERENCE   3  (bases 1 to 9527)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2013) Wuhan University, College of Life Sciences, Luo Jia 
            Shan, Wuhan, Hubei 430072, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    141..165
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(246..420)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(463..1485)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(1553..2230)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     promoter        2532..2877
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        3130..3232
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3263..3565
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     terminator      3756..4008
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(4061..4094)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4216..4232)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4435..4459
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4875..5501
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5938..7002
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7071..7265
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7384..7524
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7710..8298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8388..9179)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tagaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
       61 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
      121 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
      181 caacttaata acacattgcg gacgttttta atgttagact gaattaacgc cgaattaatt
      241 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
      301 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
      361 aaccctatag gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa
      421 ctcgagcttg tcgatcgaca gatccggtcg gcatctactc tatttctttg ccctcggacg
      481 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
      541 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
      601 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
      661 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag
      721 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
      781 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
      841 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
      901 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
      961 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     1021 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     1081 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc
     1141 gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     1201 tgcacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc
     1261 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     1321 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     1381 acgagattct tcgccctccg agagctgcat caggtcggag acactgtcga acttttcgat
     1441 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc
     1501 cggatctgcg aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg
     1561 tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag gatagtggga
     1621 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     1681 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     1741 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     1801 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     1861 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     1921 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttatc
     1981 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     2041 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     2101 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     2161 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     2221 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     2281 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctgca
     2341 ggtctagaca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
     2401 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
     2461 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
     2521 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
     2581 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
     2641 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
     2701 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     2761 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     2821 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg
     2881 gggactttgg atcccagcag cagccacagc aaaatttggt ttgggatagg taggtgttat
     2941 gttaggtctg gttttttggc tgtagcagca gcagagagac caattctcga ctaagttggc
     3001 agcatcaccc gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga
     3061 ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa
     3121 atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     3181 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aacttttgct
     3241 gacgagaaca gggactggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     3301 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcctgggc gacggatggt
     3361 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     3421 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     3481 atccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     3541 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca ggtcgacgga
     3601 ctcaacgagc ccttggtctc attcctgctg ctaggctgtt ctgtggaagt ttgcagagtt
     3661 tatattatgg gtttaatcgt ccatggcatc agcatcagca gcggtacctc tgtcttcagt
     3721 actgggcccg aagactgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     3781 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     3841 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     3901 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     3961 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gccatccgca
     4021 ctgtagcgga tggcctaaaa aaaaaactag cgtcgcagtc ataacttcgt atagcataca
     4081 ttatacgaag ttatgggccg cattaccctg ttatccctag gccgcataac ttcgtatagc
     4141 ctacattata ggatggaggg atatcctctc ttaaggtagc gagcaagctc taagaggagt
     4201 gtcgacaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4261 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4321 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
     4381 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
     4441 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
     4501 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
     4561 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
     4621 cgttcagtgc aggagatgat cgcggccggg tacgtgttcg agccgcccgc gcatgtctca
     4681 accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg
     4741 gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag
     4801 taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata
     4861 cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga
     4921 cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag
     4981 tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc
     5041 taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc
     5101 gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca
     5161 aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg
     5221 ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg
     5281 cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc
     5341 tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag
     5401 gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg
     5461 aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga
     5521 gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa
     5581 ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt
     5641 gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac
     5701 cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga
     5761 gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg
     5821 acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg
     5881 ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt
     5941 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     6001 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     6061 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     6121 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     6181 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     6241 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     6301 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     6361 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     6421 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     6481 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     6541 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     6601 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     6661 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     6721 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     6781 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     6841 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     6901 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     6961 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     7021 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag atctctttcc tgtggatagc
     7081 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     7141 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     7201 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     7261 gcataactgt ctggccagcg cacagccgaa gctcccggat acggtcacag cttgtctgta
     7321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7381 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     7441 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     7501 gtaaggagaa aataccgcat caggcgttca tccgcttcct cgctcactga ctcgctgcgc
     7561 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     7621 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7681 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7741 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7801 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7861 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7921 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7981 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8041 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8101 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     8161 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8221 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8281 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8341 aacgaaaact cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc
     8401 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     8461 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
     8521 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
     8581 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
     8641 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcctct
     8701 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
     8761 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
     8821 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
     8881 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
     8941 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
     9001 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
     9061 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
     9121 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
     9181 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     9241 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     9301 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     9361 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     9421 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     9481 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgt
//
