LOCUS       40924_2413        6038 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p1168huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    84..724
                     /label=5' flanking region of hIL6
                     /note="5' flanking region of hIL6"
     regulatory      725..1260
                     /label=hIL6 promoter
                     /note="hIL6 promoter"
                     /regulatory_class="promoter"
     CDS             1308..2957
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(3001..3122)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3541..4129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4303..5160)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5161..5265)
                     /label=AmpR promoter
     rep_origin      5292..5747
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5878..5926
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5940..6031
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgc
       61 atgcctgcag gtcgactcta gaggatcctc ctgcaagaga caccatcctg agggaagagg
      121 gcttctgaac cagcttgacc caataagaaa ttcttgggtg ccgacgcgga agcagattca
      181 gagcctagag ccgtgcctgc gtccgtagtt tccttctagc ttcttttgat ttcaaatcaa
      241 gacttacagg gagagggagc gataaacaca aactctgcaa gatgccacaa ggtcctcctt
      301 tgacatcccc aacaaagagg tgagtagtat tctccccctt tctgccctga accaagtggg
      361 cttcagtaat ttcagggctc caggagacct ggggcccatg caggtgcccc agtgaaacag
      421 tggtgaagag actcagtggc aatggggaga gcactggcag cacaaggcaa acctctggca
      481 cagagagcaa agtcctcact gggaggattc ccaaggggtc acttgggaga gggcagggca
      541 gcagccaacc tcctctaagt gggctgaagc aggtgaagaa agtggcagaa gccacgcggt
      601 ggcaaaaagg agtcacacac tccacctgga gacgccttga agtaactgca cgaaatttga
      661 ggatggccag gcagttctac aacagccgct cacagggaga gccagaacac agaagaactc
      721 agatgactgg tagtattacc ttcttcataa tcccaggctt ggggggctgc gatggagtca
      781 gaggaaactc agttcagaac atctttggtt tttacaaata caaattaact ggaacgctaa
      841 attctagcct gttaatctgg tcactgaaaa aaaatttttt ttttttcaaa aaacatagct
      901 ttagcttatt ttttttctct ttgtaaaact tcgtgcatga cttcagcttt actctttgtc
      961 aagacatgcc aaagtgctga gtcactaata aaagaaaaaa agaaagtaaa ggaagagtgg
     1021 ttctgcttct tagcgctagc ctcaatgacg acctaagctg cacttttccc cctagttgtg
     1081 tcttgcgatg ctaaaggacg tcacattgca caatcttaat aaggtttcca atcagcccca
     1141 cccgctctgg ccccaccctc accctccaac aaagatttat caaatgtggg attttcccat
     1201 gagtctcaat attagagtct caacccccaa taaatatagg actggagatg tctgaggctc
     1261 attctgccct cgaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca
     1321 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     1381 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     1441 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     1501 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     1561 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     1621 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     1681 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     1741 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     1801 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     1861 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     1921 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     1981 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     2041 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     2101 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     2161 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     2221 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     2281 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     2341 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     2401 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     2461 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     2521 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     2581 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     2641 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     2701 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     2761 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     2821 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     2881 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     2941 gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     3001 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3061 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3121 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     3181 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
     3241 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3301 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3361 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3421 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3961 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4201 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4261 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4321 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4381 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4441 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4501 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4561 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4621 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4681 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4741 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4801 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4861 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4921 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4981 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5041 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5101 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5161 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5221 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5281 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5341 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5401 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5461 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5521 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5581 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5641 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5701 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt
     5761 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     5821 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     5881 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     5941 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     6001 agtgcaagtg caggtgccag aacatttctc tatcgata
//
