LOCUS       40924_2403        5982 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p1168hIL6mIgkB-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5982)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5982)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..672
                     /label=5' FR of hIL6
                     /note="5' FR of hIL6"
     regulatory      673..1208
                     /label=hIL6 promoter mutant
                     /note="hIL6 promoter mutant"
                     /regulatory_class="promoter"
     CDS             1256..2905
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2949..3070)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3485..4073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4247..5104)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5105..5209)
                     /label=AmpR promoter
     rep_origin      5236..5691
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5822..5870
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5884..5975
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggac
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga
     1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
     1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
     1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
     3181 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     3241 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     4381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     5221 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc
     5701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     5761 agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg
     5821 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
     5881 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     5941 ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta
//
