LOCUS       40924_2388        5990 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p1168hIL6mCRE-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..672
                     /label=5' FR of hIL6
                     /note="5' FR of hIL6"
     regulatory      673..1208
                     /label=hIL6 promoter mutant
                     /note="hIL6 promoter mutant"
                     /regulatory_class="promoter"
     CDS             1256..2905
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2949..3070)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3493..4081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4255..5112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5113..5217)
                     /label=AmpR promoter
     rep_origin      5244..5699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5830..5878
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5892..5983
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc
     1021 tagttgtgtc ttgcgatgct aaagggatcc acattgcaca atcttaataa ggtttccaat
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga
     1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
     1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
     1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
     1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
     3181 gatcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     3241 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     3301 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3901 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4021 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4081 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4141 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4201 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4261 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4321 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4381 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4441 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4501 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4561 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4621 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4681 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4741 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4801 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4861 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4921 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4981 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5041 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5101 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5221 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5581 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5641 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     5701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5761 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg
     5821 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     5881 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     5941 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata
//
