LOCUS       40924_2373        4569 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p110huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4569)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4569)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             54..1703
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1747..1868)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2287..2875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3049..3906)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3907..4011)
                     /label=AmpR promoter
     polyA_signal    4038..4172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      complement(4253..4349)
                     /label=SV40 polyA late
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      4253..4349
                     /label=SV40 polyA early
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     polyA_signal    4275..4409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4448..4558
                     /label=hIL6 promoter fragment
                     /note="hIL6 promoter fragment"
                     /regulatory_class="promoter"
ORIGIN
        1 gagatctgcg atctaagtaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
       61 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
      121 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
      181 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
      241 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
      301 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
      361 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
      421 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
      481 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
      541 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
      601 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
      661 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
      721 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
      781 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
      841 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
      901 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
      961 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     1021 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     1081 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     1141 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     1201 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     1261 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     1321 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     1381 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     1441 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     1501 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     1561 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     1621 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     1681 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
     1741 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1801 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1861 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1921 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     1981 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     2041 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     2101 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2161 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2221 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2281 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2341 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2401 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2461 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2521 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2581 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2641 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2701 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2761 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2821 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2881 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2941 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3001 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3061 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3121 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3181 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3241 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3301 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3361 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3421 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3481 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3541 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3601 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3661 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3721 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3781 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3841 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3901 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3961 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4021 ccgaaaagtg ccacctgaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     4081 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4141 aactcatcaa tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg
     4201 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     4261 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     4321 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     4381 tcatcaatgt atcttatcat gtctggatct cgaagcttgc atgcctgcag gtcgactcta
     4441 gaggatcgcc ccaccctcac cctccaacaa agatttatca aatgtgggat tttcccatga
     4501 gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc tgaggctcat
     4561 tctgccctc
//
