LOCUS       40924_2358        6732 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1438..3399
                     /codon_start=1
                     /label=env
                     /note="murine leukemia virus gPr80 envelope polyprotein 
                     precursor"
                     /translation="MARSTLSKPPQDKINPWKPLIVMGVLLGVGMAESPHQVFNVTWRV
                     TNLMTGRTANATSLLGTVQDAFPKLYFDLCDLVGEEWDPSDQEPYVGYGCKYPAGRQRT
                     RTFDFYVCPGHTVKSGCGGPGEGYCGKWGCETTGQAYWKSTSSWDLISLKRGNTPWDTG
                     CSKVACGPCYDLSKVSNSFQGATRGGRCNPLVLEFTDAGKKANWDGPKSWGLRLYRTGT
                     DPITMFSLTRQVLNVGPRVPIGPNPVLPDQRLPSSPIEIVPAPQPPSPLNTSYPPSTTS
                     TPSTSPTSPSVPQPPPGTGDRLLALVKGAYQALNLTNPDKTQECWLCLVSGPPYYEGVA
                     VVGTYTNHSTAPANCTATSQHKLTLSEVTGQGLCMGAVPKTHQALCNTTQSAGSGSYYL
                     AAPAGTMWACSTGLTPCLSTTVLNLTTDYCVLVELWPRVIYHSPDYMYGQLEQRTKYKR
                     EPVSLTLALLLGGLTMGGIAAGIGTGTTALIKTQQFEQLHAAIQTDLNEVEKSITNLEK
                     SLTSLSEVVLQNRRGLDLLFLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLNQRQ
                     KLFETGQGWFEGLFNRSPWFTTLISTIMGPLIVLLLILLFGPCILNRLVQFVKDRISVV
                     QALVLTQQYHQLKPIEYEP"
     polyA_signal    3502..3557
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3915..3931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3939..3955)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3963..3993)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4008..4029)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4317..4905)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5079..5936)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5937..6041)
                     /label=AmpR promoter
     rep_origin      complement(6067..6522)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6664..6680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6690..6708
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
      721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatcc gccaccaatg
     1441 gcgcgttcaa cgctctcaaa accccctcaa gataagatta acccgtggaa gcccttaata
     1501 gtcatgggag tcctgttagg agtagggatg gcagagagcc cccatcaggt ctttaatgta
     1561 acctggagag tcaccaacct gatgactggg cgtaccgcca atgccacctc cctcctggga
     1621 actgtacaag atgccttccc aaaattatat tttgatctat gtgatctggt cggagaggag
     1681 tgggaccctt cagaccagga accgtatgtc gggtatggct gcaagtaccc cgcagggaga
     1741 cagcggaccc ggacttttga cttttacgtg tgccctgggc ataccgtaaa gtcggggtgt
     1801 gggggaccag gagagggcta ctgtggtaaa tgggggtgtg aaaccaccgg acaggcttac
     1861 tggaagtcca catcatcgtg ggacctaatc tcccttaagc gcggtaacac cccctgggac
     1921 acgggatgct ctaaagttgc ctgtggcccc tgctacgacc tctccaaagt atccaattcc
     1981 ttccaagggg ctactcgagg gggcagatgc aaccctctag tcctagaatt cactgatgca
     2041 ggaaaaaagg ctaactggga cgggcccaaa tcgtggggac tgagactgta ccggacagga
     2101 acagatccta ttaccatgtt ctccctgacc cggcaggtcc ttaatgtggg accccgagtc
     2161 cccatagggc ccaacccagt attacccgac caaagactcc cttcctcacc aatagagatt
     2221 gtaccggctc cacagccacc tagccccctc aataccagtt accccccttc cactaccagt
     2281 acaccctcaa cctcccctac aagtccaagt gtcccacagc cacccccagg aactggagat
     2341 agactactag ctctagtcaa aggagcctat caggcgctta acctcaccaa tcccgacaag
     2401 acccaagaat gttggctgtg cttagtgtcg ggacctcctt attacgaagg agtagcggtc
     2461 gtgggcactt ataccaatca ttccaccgct ccggccaact gtacggccac ttcccaacat
     2521 aagcttaccc tatctgaagt gacaggacag ggcctatgca tgggggcagt acctaaaact
     2581 caccaggcct tatgtaacac cacccaaagc gccggctcag gatcctacta ccttgcagca
     2641 cccgccggaa caatgtgggc ttgcagcact ggattgactc cctgcttgtc caccacggtg
     2701 ctcaatctaa ccacagatta ttgtgtatta gttgaactct ggcccagagt aatttaccac
     2761 tcccccgatt atatgtatgg tcagcttgaa cagcgtacca aatataaaag agagccagta
     2821 tcattgaccc tggcccttct actaggagga ttaaccatgg gagggattgc agctggaata
     2881 gggacgggga ccactgcctt aattaaaacc cagcagtttg agcagcttca tgccgctatc
     2941 cagacagacc tcaacgaagt cgaaaagtca attaccaacc tagaaaagtc actgacctcg
     3001 ttgtctgaag tagtcctaca gaaccgcaga ggtctagatt tgctattcct aaaggaggga
     3061 ggtctctgcg cagccctaaa agaagaatgt tgtttttatg cggaccacac ggggctagtg
     3121 agagacagca tggccaaatt aagagaaagg cttaatcaga gacaaaaact atttgagaca
     3181 ggccaaggat ggttcgaagg gctgtttaat agatccccct ggtttaccac cttaatctcc
     3241 accatcatgg gacctctaat agtactctta ctgatcttac tctttggacc ttgcattctc
     3301 aatcgattag tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact
     3361 caacaatatc accagctgaa acccatagag tacgagccat gatggccaat gccctggctc
     3421 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3481 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3541 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3601 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3661 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3721 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3781 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3841 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ggatcggccg
     3901 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3961 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4021 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4081 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4141 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4201 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4261 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4321 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4381 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4441 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4501 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4561 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4621 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4681 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4741 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4801 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4861 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4921 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4981 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5041 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5101 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5161 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5221 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5281 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5341 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5401 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5461 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5521 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5581 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5641 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5701 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5761 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5821 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5881 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5941 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6001 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6061 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6121 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6181 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6241 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6301 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6361 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6421 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6481 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     6541 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6601 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6661 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc
     6721 tccaccgcgg tg
//
