LOCUS       40924_2338        7006 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector p06-PgapA-cre, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7006)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ, 
            Krylov AA, Mashko SV.
  TITLE     Mu-driven transposition of recombinant mini-Mu unit DNA in the 
            Corynebacterium glutamicum chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7006)
  AUTHORS   Gorshkova NV, Lobanova JS, Tokmakova IL, Smirnov SV, Akhverdyan VZ, 
            Krylov AA, Mashko SV.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st 
            Dorozhny pr. 1-1, Moscow 117545, Russian Federation
REFERENCE   3  (bases 1 to 7006)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7006)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-2017) Ajinomoto-Genetika Research Institute, 1st Dorozhny 
            pr. 1-1, Moscow 117545, Russian Federation"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(928..1584)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(1585..1687)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2213..2758)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     terminator      2951..3037
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3129..3156
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      3190..3296
                     /label=PdapA
                     /note="PdapA"
                     /regulatory_class="promoter"
     CDS             3315..4343
                     /label=Cre
                     /note="site-specific recombinase"
     rep_origin      complement(5171..6628)
                     /direction=LEFT
                     /label=ori pCG1
                     /note="ori pCG1"
ORIGIN
        1 gcgcgcgtgg gctagggctt aacgcgcgtt ttgccgtgct gcggggcata cgttagcgca
       61 tacgcttttt tctgtgaaac ctttttgtgt tgttgtttcg tgttggtttc ctttctgttg
      121 gcggggcaac ttaacgcctg cgggggtggt tgttgacgtt aacgggggta gtttttattc
      181 ccctagtggt ttttcagtac gacaatcgag aaagacctgt ttcagccagt tcgggtcatg
      241 ttcgtcggta tggccacgtg catagcgacc agttttcgag ttcactggga tttttggtgc
      301 atcgaacaag atgtaggaca atgcggtttc taggtctact ttttgcttta tgccgtacaa
      361 gccccgtggg tattcagcga ttgattccaa ggcggcttcc cagtcctgtt ttgtgaagga
      421 ctggcttagt tctaggtctg tgtctgggta gtactgcttg tttgtgtaag cgccgttggt
      481 gctcattgat gattcctttg aagtgtttgg agttcggcta gtagtgcggc gtatggtgct
      541 gctttttgct cgtgatagct cgccttggct atgaggtcgg ctaggtaggt ttccggggtg
      601 cctaggttgc gtaggtctag caaatcccgg tatgtggcct gtgcgctgcg ctggtggtgc
      661 atacagtcgt taagctgggc ttttacgtct gcgatgcggt ggcggttagg catgttggtg
      721 tgcttcttcc aagtactcac gggcgggttt tgtgtatgcc tggcgtgatg cttctttgag
      781 ctgttggagt tccgcttgga gtgcgggtag ttcgtccgcg aactgcttgt ggtactcgta
      841 tttctcttgt tcctgggcga tcagatttgc gttgaattgc agggcggtga gttcgtccac
      901 gcgccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg
      961 taattcatta agcattctgc cgacatggaa gccatcacag acggcatgat gaacctgaat
     1021 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1081 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1141 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1201 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1261 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1321 aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc
     1381 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1441 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1501 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     1561 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     1621 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     1681 ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caacagggac
     1741 accaggattt atttattctg cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag
     1801 tgcgtcgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt tgaggtgctc
     1861 cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg gtggtgcgta
     1921 acggcaaaag caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg
     1981 cactgatgag ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg
     2041 cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc
     2101 tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa
     2161 gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata
     2221 ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc
     2281 cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     2341 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca
     2401 cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc
     2461 cccgttcagt ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa
     2521 agacatgcaa aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt
     2581 gaagtcatgc gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc
     2641 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg
     2701 caaggcggtt ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga
     2761 agatcatctt attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa
     2821 tgtagcacct gaagtcagcc ccatacgata taagttgtaa ttctcatgtt tgaacgcgta
     2881 ggcttcagag cgatggcccc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     2941 tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     3001 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     3061 tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     3121 aattaagcag aaggccatcc tgacggatgg cctttttgcg tggccagtgc caagcttgca
     3181 tgcctgcagc ttgggcgatt gttatgcaaa agttgttagg ttttttgcgg ggttgtttaa
     3241 cccccaaatg agggaagaag gtaaccttga actctatgag cacaggttta acagctgtcg
     3301 acaggaggtg ttaaatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
     3361 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
     3421 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcgtgg gcggcatggt
     3481 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
     3541 tatatcttca ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa
     3601 acatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg
     3661 ttatgcggcg gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag
     3721 cgttcgaacg cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc
     3781 aggatatacg taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
     3841 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
     3901 atattggcag aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg
     3961 gggtaactaa actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata
     4021 actacctgtt ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
     4081 tatcaactcg cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta
     4141 aggatgactc tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg
     4201 cgcgagatat ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga
     4261 ccaatgtaaa tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg
     4321 tgcgcctgct ggaagatggc gattagccat taacgcgtaa atgattgcta taattatttg
     4381 atatttatgg gtaccgagct cgaattctca tgtttgacag cttatcactg atcagtgaat
     4441 taatggcgat gacgcatcct cacgataata tccgggtagg cgcaatcact ttcgtctcta
     4501 ctccgttaca aagcgaggct gggtatttcc cggcctttct gttatccgaa atccactgaa
     4561 agcacagcgc gtgcggtgac cacgacgggc ttagcctttg cctgcgcttc tagtgcttcg
     4621 atggtggctt gtgcctgcgc ttgctgcgcc tgtagtgcct gttgagcttc ttgtagttgc
     4681 tgttctagct gtgccttggt tgccatgctt taagactcta gtagctttcc tgcgatatgt
     4741 catgcgcatg cgtagcaaac attgtcctgc aactcattca ttatgtgcag tgctcctgtt
     4801 actagtacca ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga
     4861 tgagagcttt gttgtaggtg gacggggatc ttttctacgg ggtctgacgc tcagtggaac
     4921 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatcat gcgagcaacc
     4981 tccataagat cagctaggcg atctttggga gcagtccttg tcgcgttacg aggtgagccg
     5041 gtggggaacc gttagctgcc tatggtgtga gcccccctag agagcttcaa gagcaatcag
     5101 cccgacctag aaaggaggcc aagagagaga cctacggggg gaaccgtttt ctgcctacga
     5161 gatgggcaca ttactgggaa gctttacggc gtcctcgtgg aagttcaatg cccgcagact
     5221 taagtgctct attcacggtc tgacgtgaca cgctaaattc agacatagct tcattgattg
     5281 tcggccacga gccagtctct ccctcaacag tcataaacca acctgcaatg gtcaagcgat
     5341 ttcctttagc tttcctagct tgtcgttgac tggacttagc tagtttttct cgctgtgctc
     5401 gggcgtactc actgtttggg tctttccagc gttctgcggc ctttttaccg ccacgtcttc
     5461 ccatagtggc cagagctttt cgccctcggc tgctctgcgt ctctgtctga cgagcaggga
     5521 cgactggctg gcctttagcg acgtagccgc gcacacgtcg cgccatcgtc tggcggtcac
     5581 gcatcggcgg cagatcaggc tcacggccgt ctgctccgac cgcctgagcg acggtgtagg
     5641 cacgctcgta ggcgtcgatg atcttggtgt cttttaggcg ctcaccagcc gcttttaact
     5701 ggtatcccac agtcaaagcg tggcgaaaag ccgtctcatc acgggcggca cgccctggag
     5761 cagtccagag gacacggacg ccgtcgatca gctctccaga cgcttcagcg gcgctcggca
     5821 ggcttgcttc aagcgtggca agtgcttttg cttccgcagt ggcttttctt gccgcttcga
     5881 tacgtgcccg tccgctagaa aactcctgct catagcgttt tttaggtttt tctgtgcctg
     5941 agatcatgcg agcaacctcc ataagatcag ctaggcgatc cacgcgattg tgctgggcat
     6001 gccagcggta cgcggtggga tcgtcggaga cgtgcagtgg ccaccggctc agcctatgtg
     6061 aaaaagcctg gtcagcgccg aaaacgcggg tcatttcctc ggtcgttgca gccagcaggc
     6121 gcatattcgg gctgctcatg cctgctgcgg catacaccgg atcaatgagc cagatgagct
     6181 ggcatttccc gctcagtgga ttcacgccga tccaagctgg cgctttttcc aggcgtgccc
     6241 agcgctccaa aatcgcgtag acctcggggt ttacgtgctc gattttcccg ccggcctggt
     6301 ggctcggcac atcaatgtcc aggacaagca cggctgcgtg ctgcgcgtgc gtcagagcaa
     6361 catactggca ccgggcaagc gattttgaac caactcggta taacttcggc tgtgtttctc
     6421 ccgtgtccgg gtctttgatc caagcgctgg cgaagtcgcg ggtcttgctg ccctggaaat
     6481 tttctctgcc caggtgagcg aggaattcgc ggcggtcttc gctcgtccag ccacgtgatc
     6541 gcagcgcgag ctcgggatgg gtgtcgaaca gatcagcgga aaatttccag gccggtgtgt
     6601 caatgtctcg tgaatccgct agagtcattt ttgagcgctt tctcccaggt ttggactggg
     6661 ggttagccga cgccctgtga gttaccgctc acggggcgtt caacattttt caggtattcg
     6721 tgcagcttat cgcttcttgc cgcctgtgcg ctttttcgac gcgcgacgct gctgccgatt
     6781 cggtgcaggt ggtggcggcg ctgacacgtc ctgggcggcc acggccacac gaaacgcggc
     6841 atttacgatg tttgtcatgc ctgcgggcac cgcgccacga tcgcggataa ttctcgctgc
     6901 cgcttccagc tctgtgacga ccatggccaa aatttcgctc gggggacgca cttccagcgc
     6961 catttgcgac ctagccgcct ccagctcctc ggcgtggcgt ttgttg
//
