LOCUS       40924_2303        4963 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector p(Gal4)2-50huIL6P-luc+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4963)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4963)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      32..48
                     /label=synthetic GAL4 upstream activator sequence
                     /note="synthetic GAL4 upstream activator sequence"
                     /regulatory_class="promoter"
     regulatory      53..69
                     /label=synthetic GAL4 upstream activator sequence
                     /note="synthetic GAL4 upstream activator sequence"
                     /regulatory_class="promoter"
     regulatory      132..181
                     /label=hIL6 promoter fragment
                     /note="hIL6 promoter fragment"
                     /regulatory_class="promoter"
     CDS             229..1878
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1922..2043)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2466..3054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3228..4085)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4086..4190)
                     /label=AmpR promoter
     rep_origin      4217..4672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4803..4851
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4865..4956
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gcggaggacn gtcctccgga tccggaggac
       61 ngtcctccgc ccgggctcga gatctgcgat ctaagtaagc ttgcatgcct gcaggtcgac
      121 tctagaggat cattagagtc tcaaccccca ataaatatag gactggagat gtctgaggct
      181 cattctgccc tcgaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc
      241 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
      301 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
      361 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
      421 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
      481 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
      541 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
      601 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
      661 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
      721 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
      781 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
      841 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
      901 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
      961 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
     1021 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
     1081 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     1141 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     1201 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     1261 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     1321 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     1381 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     1441 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     1501 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     1561 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     1621 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     1681 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     1741 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     1801 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     1861 ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg
     1921 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1981 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     2041 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     2101 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgatcgac
     2161 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2221 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2641 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     2941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3121 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3181 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3241 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3301 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3361 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3421 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3481 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3541 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3601 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3661 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3721 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3781 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3841 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3901 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3961 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4021 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4081 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4141 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4201 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4261 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4561 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4621 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccattcg
     4681 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4741 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     4801 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     4861 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     4921 tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
