LOCUS       40924_1974        5610 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integration vector mini-CTX1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     A site-specific gene integration system for Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-1999) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 5610)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5610)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-1999) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    858..965
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(974..992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(1166..1213)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            complement(1420..1528)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(2953..3541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4366..5553)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(join(5601..5610,1..19))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatccc
      901 ccgggctgca ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      961 gtacccaatt cgccctatag tgagtcgtat tacgcgcgcc ggtggatgac cttttgaatg
     1021 acctttaata gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt
     1081 aaaaattttt tcacaaaacg gtttacaagc ataaagctaa ttccattgag taagttttta
     1141 agcacatcag cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg
     1201 gaataggaac ttcaagatcc ccaattcgct gcattaatga atcggccaac gcgcggggag
     1261 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1321 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1381 atcaggggat aacgcaggaa agaacatgct agagcttatc ggccagcctc gcagagcagg
     1441 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     1501 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     1561 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     1621 aatcgctata atgaccccga agcagggtta tgcagcggaa agtatacctt aacctttttc
     1681 gttgcttcag gaaagtttca gcgcctgatt cagcagacct acgatgtcgg ggccgtcctt
     1741 gctgatccac ttggcgtaat gcttgaagat catcgccgtc gaggtgtgcc ccatctggtc
     1801 cgcgatccat tcgggagtgg cgatgccgct gctgagcatc tggctggcga aggtgtggcg
     1861 gcactgattg ggaccacgcg agcggacgcc cgcattcctg aggtggccat gccaccagcc
     1921 gttgcgcagc gtatccgacg tggagtacgc cgccccgcta gcactgttgt ggaacacgaa
     1981 gcgtacccgt tgaggctttc tggtccggtt gtcgcggtct atcacctcga tctgtacggg
     2041 cggaaggcgt ctggttagct tggcctgctg ctggagtgcg cgcagcgccg gggccagtag
     2101 ctggaccttc ctcgtcgatc ggcgagtctt ggtgaccttg tactggctgc gtacacgagc
     2161 gcggcggaaa accacggtcc ccgtgtccaa atcgacatct tcccaggcca gagctatggc
     2221 ctcgctgacg cgggggccgc tccagatcat gaactccgcc aggttcagct caccgatgcg
     2281 cgcggtttca gtaccgagga tgaggtcgat ttcctcacga gtgaatgggt ctgggtcatc
     2341 cgcatccgga agagtgatca cgatgccgtc tgttgggtcg tgcgcgctcc ggttccgagt
     2401 tcgatacagc cggaagatct gccgcaggtt gctgacgatc tctcgcaccg tcttgttgtg
     2461 cagcttgggc atgagcgtgt tctgcaccca gtcttgaata tccaggtggt cgatgctgtc
     2521 ggcctggctg tcaccccagc gaggtcggat gtgggtttcc acgcggctgg tgtagccgcg
     2581 gaagccgctg gcggcaatct ggttgcgttt gatgtcgagc cagaggtcga tgtaatggcc
     2641 caaggtgttg cttttcaccc gcggtgagtc cgggaagtgt cggctgtagc tgaagactcc
     2701 ttgcttgatc tcgtagttga tgatgccggc cagccgttcg gcattggcta tattctcggg
     2761 cgttgcgtcc cctggtatgg actccctaca cagctcgccc tggtagcgga agtagatcct
     2821 gatccgtttt ccgcgtacct cgactccgtc tgccatctgg ttcccctgat ccacaccaag
     2881 cgttcaagct ggcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3361 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4261 tgagtcaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc
     4321 ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg
     4381 gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat
     4441 ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg
     4501 tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg
     4561 gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga
     4621 agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac
     4681 gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     4741 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     4801 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     4861 cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac
     4921 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     4981 cggtcggcgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     5041 gccgttgagc accgccgccg caaggaatgg tgcatgtaag gagatggcgc ccaacagtcc
     5101 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     5161 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     5221 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt
     5281 cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg
     5341 gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata
     5401 tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacaa tatcccgcaa
     5461 gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc
     5521 gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt
     5581 taactgtgat aaactaccgc attaaagcta
//
