LOCUS       40924_1969        8875 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Integration vector mini-CTX-lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     A site-specific gene integration system for Pseudomonas aeruginosa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Hoang TT, Kutchma AJ, Becher A, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-1999) Microbiology, Colorado State University, 
            Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 8875)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8875)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-APR-1999) Microbiology, Colorado State University, Fort Collins,
            CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        827..845
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(924..3995)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(4197..4213)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4239..4257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4431..4478)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     oriT            complement(4685..4793)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(6218..6806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7631..8818)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     promoter        complement(join(8866..8875,1..19))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 atcgatgata agctgtcaaa catgagaatt aattcatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
      181 ctctccccgc gcgttggccg attcattaat gcagcgaatt agcttcaaaa gcgctctgaa
      241 gttcctatac tttctagaga ataggaactt cggaatagga acttcaagat cccctgattc
      301 cctttgtcaa cagcaatgga ttttcaatgg gtcaacatga aatccggctg ttgccctggg
      361 acggcaggtt tgacggcagg cttccaggct ctctcgcaca cgctgggaac actctgggaa
      421 caaacgcgag tgaaaatgga cagtagggcc caggaataat gcggcctcca gcgaggttgg
      481 ggcgtttcat atgagttcga atctcaccgc ctccgccata taacctgctg ttttcattga
      541 tgtttccatt cgacccactc tcaggagtga acgatttggg aacaaactgg gaacgaggcg
      601 cagaaaaaag gggccccgca gggccccttt ttcgttgctt caggaaagtt tcagcgcctg
      661 attcagcaga cctacgatgt cggggccgtc cttgctgaat tagctttatg cttgtaaacc
      721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
      781 aatataatct attaaaggtc attcaaaagg tcatccaccg gcgcgcaatt aaccctcact
      841 aaagggaaca aaagctggaa atagtacata atggatttcc ttacgcgaaa tacgggcaga
      901 catggcctgc ccggttatta ttatttttga caccagacca actggtaatg gtagcgaccg
      961 gcgctcagct ggaactccgc cgatactgac gggctccagg agtcgtcgcc accaatcccc
     1021 atatggaaac cgtcgatatt cagccatgtg ccttcttccg cgtgcagcag atggcgatgg
     1081 ctggtttcca tcagttgctg ttgactgtag cggctgatgt tgaactggaa gtcgccgcgc
     1141 cactggtgtg ggccataatt caattcgcgc gtcccgcagc gcagaccgtt ttcgctcggg
     1201 aagacgtacg gggtatacat gtctgacaat ggcagatccc agcggtcaaa acaggcggca
     1261 gtaaggcggt cgggatagtt ttcttgcggc cctaatccga gccagtttac ccgctctgct
     1321 acctgcgcca gctggcagtt caggccaatc cgcgccggat gcggtgtatc gctcgccact
     1381 tcaacatcaa cggtaatcgc catttgacca ctaccatcaa tccggtaggt tttccggctg
     1441 ataaataagg ttttcccctg atgctgccac gcgtgagcgg tcgtaatcag caccgcatca
     1501 gcaagtgtat ctgccgtgca ctgcaacaac gctgcttcgg cctggtaatg gcccgccgcc
     1561 ttccagcgtt cgacccaggc gttagggtca atgcgggtcg cttcacttac gccaatgtcg
     1621 ttatccagcg gtgcacgggt gaactgatcg cgcagcggcg tcagcagttg ttttttatcg
     1681 ccaatccaca tctgtgaaag aaagcctgac tggcggttaa attgccaacg cttattaccc
     1741 agctcgatgc aaaaatccat ttcgctggtg gtcagatgcg ggatggcgtg ggacgcggcg
     1801 gggagcgtca cactgaggtt ttccgccaga cgccactgct gccaggcgct gatgtgcccg
     1861 gcttctgacc atgcggtcgc gttcggttgc actacgcgta ctgtgagcca gagttgcccg
     1921 gcgctctccg gctgcggtag ttcaggcagt tcaatcaact gtttaccttg tggagcgaca
     1981 tccagaggca cttcaccgct tgccagcggc ttaccatcca gcgccaccat ccagtgcagg
     2041 agctcgttat cgctatgacg gaacaggtat tcgctggtca cttcgatggt ttgcccggat
     2101 aaacggaact ggaaaaactg ctgctggtgt tttgcttccg tcagcgctgg atgcggcgtg
     2161 cggtcggcaa agaccagacc gttcatacag aactggcgat cgttcggcgt atcgccaaaa
     2221 tcaccgccgt aagccgacca cgggttgccg ttttcatcat atttaatcag cgactgatcc
     2281 acccagtccc agacgaagcc gccctgtaaa cggggatact gacgaaacgc ctgccagtat
     2341 ttagcgaaac cgccaagact gttacccatc gcgtgggcgt attcgcaaag gatcagcggg
     2401 cgcgtctctc caggtagcga aagccatttt ttgatggacc atttcggcac agccgggaag
     2461 ggctggtctt catccacgcg cgcgtacatc gggcaaataa tatcggtggc cgtggtgtcg
     2521 gctccgccgc cttcatactg caccgggcgg gaaggatcga cagatttgat ccagcgatac
     2581 agcgcgtcgt gattagcgcc gtggcctgat tcattcccca gcgaccagat gatcacactc
     2641 gggtgattac gatcgcgctg caccattcgc gttacgcgtt cgctcatcgc cggtagccag
     2701 cgcggatcat cggtcagacg attcattggc accatgccgt gggtttcaat attggcttca
     2761 tccaccacat acaggccgta gcggtcgcac agcgtgtacc acagcggatg gttcggataa
     2821 tgcgaacagc gcacggcgtt aaagttgttc tgcttcatca gcaggatatc ctgcaccatc
     2881 gtctgctcat ccatgacctg accatgcaga ggatgatgct cgtgacggtt aacgcctcga
     2941 atcagcaacg gcttgccgtt cagcagcagc agaccatttt caatccgcac ctcgcggaaa
     3001 ccgacatcgc aggcttctgc ttcaatcagc gtgccgtcgg cggtgtgcag ttcaaccacc
     3061 gcacgataga gattcgggat ttcggcgctc cacagtttcg ggttttcgac gttcagacgt
     3121 agtgtgacgc gatcggcata accaccacgc tcatcgataa tttcaccgcc gaaaggcgcg
     3181 gtgccgctgg cgacctgcgt ttcaccctgc cataaagaaa ctgttacccg taggtagtca
     3241 cgcaactcgc cgcacatctg aacttcagcc tccagtacag cgcggctgaa atcatcatta
     3301 aagcgagtgg caacatggaa atcgctgatt tgtgtagtcg gtttatgcag caacgagacg
     3361 tcacggaaaa tgccgctcat ccgccacata tcctgatctt ccagataact gccgtcactc
     3421 caacgcagca ccatcaccgc gaggcggttt tctccggcgc gtaaaaatgc gctcaggtca
     3481 aattcagacg gcaaacgact gtcctggccg taaccgaccc agcgcccgtt gcaccacaga
     3541 tgaaacgccg agttaacgcc atcaaaaata attcgcgtct ggccttcctg tagccagctt
     3601 tcatcaacat taaatgtgag cgagtaacaa cccgtcggat tctccgtggg aacaaacggc
     3661 ggattgaccg taatgggata ggttacgttg gtgtagatgg gcgcatcgta accgtgcatc
     3721 tgccagtttg aggggacgac gacagtatcg gcctcaggaa gatcgcactc cagccagctt
     3781 tccggcaccg cttctggtgc cggaaaccag gcaaagcgcc attcgccatt caggctgcgc
     3841 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     3901 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     3961 aaaacgacgg ccagtgaatc cgtaatcatg gtcatagctg tttcctgtgt gataaagaaa
     4021 gttaaaatgc cgcgccccta cctacctagg atctcgatcc cggtcgtatt aaccggaaga
     4081 aggtcaatca taaaggccac tcttgcgaat gaccttgagt ttgtccctct atagtgagtc
     4141 gggatcgcta gttagttagg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat
     4201 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc
     4261 gcgccggtgg atgacctttt gaatgacctt taatagatta tattactaat taattgggga
     4321 ccctagaggt cccctttttt attttaaaaa ttttttcaca aaacggttta caagcataaa
     4381 gctaattcca ttgagtaagt ttttaagcac atcagcttca aaagcgctct gaagttccta
     4441 tactttctag agaataggaa cttcggaata ggaacttcaa gatccccaat tcgctgcatt
     4501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgctagagc
     4681 ttatcggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
     4741 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
     4801 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
     4861 cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
     4921 cggaaagtat accttaacct ttttcgttgc ttcaggaaag tttcagcgcc tgattcagca
     4981 gacctacgat gtcggggccg tccttgctga tccacttggc gtaatgcttg aagatcatcg
     5041 ccgtcgaggt gtgccccatc tggtccgcga tccattcggg agtggcgatg ccgctgctga
     5101 gcatctggct ggcgaaggtg tggcggcact gattgggacc acgcgagcgg acgcccgcat
     5161 tcctgaggtg gccatgccac cagccgttgc gcagcgtatc cgacgtggag tacgccgccc
     5221 cgctagcact gttgtggaac acgaagcgta cccgttgagg ctttctggtc cggttgtcgc
     5281 ggtctatcac ctcgatctgt acgggcggaa ggcgtctggt tagcttggcc tgctgctgga
     5341 gtgcgcgcag cgccggggcc agtagctgga ccttcctcgt cgatcggcga gtcttggtga
     5401 ccttgtactg gctgcgtaca cgagcgcggc ggaaaaccac ggtccccgtg tccaaatcga
     5461 catcttccca ggccagagct atggcctcgc tgacgcgggg gccgctccag atcatgaact
     5521 ccgccaggtt cagctcaccg atgcgcgcgg tttcagtacc gaggatgagg tcgatttcct
     5581 cacgagtgaa tgggtctggg tcatccgcat ccggaagagt gatcacgatg ccgtctgttg
     5641 ggtcgtgcgc gctccggttc cgagttcgat acagccggaa gatctgccgc aggttgctga
     5701 cgatctctcg caccgtcttg ttgtgcagct tgggcatgag cgtgttctgc acccagtctt
     5761 gaatatccag gtggtcgatg ctgtcggcct ggctgtcacc ccagcgaggt cggatgtggg
     5821 tttccacgcg gctggtgtag ccgcggaagc cgctggcggc aatctggttg cgtttgatgt
     5881 cgagccagag gtcgatgtaa tggcccaagg tgttgctttt cacccgcggt gagtccggga
     5941 agtgtcggct gtagctgaag actccttgct tgatctcgta gttgatgatg ccggccagcc
     6001 gttcggcatt ggctatattc tcgggcgttg cgtcccctgg tatggactcc ctacacagct
     6061 cgccctggta gcggaagtag atcctgatcc gttttccgcg tacctcgact ccgtctgcca
     6121 tctggttccc ctgatccaca ccaagcgttc aagctggcat gtgagcaaaa ggccagcaaa
     6181 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6241 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6301 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6361 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6421 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6481 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6541 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6601 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6661 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6721 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6781 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6841 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6901 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6961 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7021 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7081 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7141 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7201 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7261 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7321 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7381 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7441 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7501 ccgtaagatg cttttctgtg actggtgagt caagggttgg tttgcgcatt cacagttctc
     7561 cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg
     7621 cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga
     7681 caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg
     7741 cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga
     7801 gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca
     7861 acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc
     7921 ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa
     7981 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     8041 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     8101 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     8161 agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     8221 ctgggttgaa ggctctcaag ggcatcggtc ggcgctctcc cttatgcgac tcctgcatta
     8281 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     8341 gtaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     8401 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     8461 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     8521 agagaatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta
     8581 gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg
     8641 cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc
     8701 tgtcggaatg gacaatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc
     8761 ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac
     8821 acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcta
//
